The effect of partial replacement of soybean meal with fermented soybean meal on chicken performance and immune status
More details
Hide details
University of Life Sciences in Lublin, Faculty of Biology, Animal Sciences and Bioeconomy, Department of Biochemistry and Toxicology, 20-950 Lublin, Poland
Publication date: 2019-09-23
Corresponding author
I. Sembratowicz   

University of Life Sciences in Lublin, Faculty of Biology, Animal Sciences and Bioeconomy, Department of Biochemistry and Toxicology, 20-950 Lublin, Poland
J. Anim. Feed Sci. 2019;28(3):263-271
The aim of the study was to evaluate the effect of partial replacement of soybean meal (SM) with 3 or 6% of fermented soybean meal (FSM) on growth performance, gut microbiota, intestinal histology and immunity of chickens. In the study one-day-old male Ross 308 chickens raised to the age of 40 days were used. The experiment was carried out on 600 animals assigned to three experimental groups of 200 birds each (10 replications of 20 individuals each). In the control group (C), SM was the main source of dietary protein, whereas the chickens from FSM-3% and FSM-6% groups were fed diets in which SM was partially replaced with FSM at a dose of 3 and 6%, respectively. It was shown that partial replacement of SM with FSM (both 3 and 6%) improved the chicken growth performance and dressing percentage, and modulated the immune system. However, the introduction of a 6% dose of FSM was found to better improve the morphology of intestines (reflected by increased villi length and crypts depth) than a 3% FSM dose. It also contributed to a decrease in total number of fungi and coliforms in the jejunum. Based on the obtained results, it can be concluded, that it is better to use a 6% share of FSM in chicken feed, than a 3% share.
Amadou I., Amza T., Foh M.B.K., Kamara M.T., Le G.-W., 2010. Influence of Lactobacillus plantarum Lp6 fermentation on the functional properties of soybean protein meal. Emir. J. Food Agric. 22, 456–465, https://doi.org/10.9755/ejfa.v....
Awad W.A., Ghareeb K., Böhm J., 2010. Effect of addition of a probiotic micro-organism to broiler diet on intestinal mucosal architecture and electrophysiological parameters. J. Anim. Physiol. Anim. Nutr. 94, 486–494, https://doi.org/10.1111/j.1439....
Canibe N., Jensen B.B., 2003. Fermented and nonfermented liquid feed to growing pigs: Effect on aspects of gastrointestinal ecology and growth performance. J. Anim. Sci. 81, 2019–2031, https://doi.org/10.2527/2003.8....
Canibe N., Jensen B.B., 2012. Fermented liquid feed – microbial and nutritional aspects and impact on enteric diseases in pigs. Anim. Feed Sci. Technol. 173, 17–40, https://doi.org/10.1016/j.anif....
Chiang G., Lu W.Q., Piao X.S., Hu J.K., Gong L.M., Thacker P.A., 2010. Effects of feeding solid-state fermented rapeseed meal on performance, nutrient digestibility, intestinal ecology and intestinal morphology of broiler chickens. Asian-Australas. J. Anim. Sci. 23, 263–271, https://doi.org/10.5713/ajas.2....
Choi Y.J., Lee S.R., Oh J.-W., 2014. Effects of dietary fermented seaweed and seaweed fusiforme on growth performance, carcass parameters and immunoglobulin concentration in broiler chicks. Asian-Australas. J. Anim. Sci. 27, 862–870, https://doi.org/10.5713/ajas.2....
Drażbo A., Mikulski D., Jankowski J., Zduńczyk Z., 2018. The effect of diets containing raw and fermented faba beans on gut functioning and growth performance in young turkeys. J. Anim. Feed Sci. 27, 65–73, https://doi.org/10.22358/jafs/....
Engberg R.M., Hammershøj M., Johansen N.F., Abousekken M.S., Steenfeldt S., Jensen B.B., 2009. Fermented feed for laying hens: effects on egg production, egg quality, plumage condition and composition and activity of the intestinal microflora. Br. Poult. Sci. 50, 228–239, https://doi.org/10.1080/000716....
Feng J., Liu X., Xu Z.R., Liu Y.Y., Lu Y.P., 2007. Effects of Aspergillus oryzae 3.042 fermented soybean meal on growth performance and plasma biochemical parameters in broilers. Anim. Feed Sci. Technol. 134, 235–242, https://doi.org/10.1016/j.anif....
Gao J., Zhang H.J., Wu S.G., Yu S.H., Yoon I., Moore D., Gao Y.P., Yan H.J., Qi G.H., 2009. Effect of Saccharomyces cerevisiae fermentation product on immune functions of broilers challenged with Eimeria tenella. Poult. Sci. 88, 2141–2151, https://doi.org/10.3382/ps.200....
Heres L., Engel B., van Knapen F., de Jong M.C., Wagenaar J.A., Urlings H.A., 2003. Fermented liquid feed reduces susceptibility of broilers for Salmonella enteritidis. Poult. Sci. 82, 603–611, https://doi.org/10.1093/ps/82.....
Heres L., Urlings H.A.P., Wagenaar J.A., de Jong M.C.M., 2004. Transmission of Salmonella between broiler chickens fed with fermented liquid feed. Epidemiol. Infect. 132, 107–116, https://doi.org/10.1017/S09502....
Hong K.-J., Lee C.-H., Kim S.W., 2004. Aspergillus oryzae GB-107 fermentation improves nutritional quality of food soybeans and feed soybean meals. J. Med. Food 7, 430–435, https://doi.org/10.1089/jmf.20....
Ibrahim K.E., Al-Mutary M.G., Bakhiet A.O., Khan H.A., 2018. Histopathology of the liver, kidney, and spleen of mice exposed to gold nanoparticles. Molecules 23, 1848, https://doi.org/10.3390/molecu....
Jazi V., Ashayerizadeh A., Toghyani M., Shabani A., Tellez G., Toghyani M., 2018. Fermented soybean meal exhibits probiotic properties when included in Japanese quail diet in replacement of soybean meal. Poult. Sci. 97, 2113–2122, https://doi.org/10.3382/ps/pey....
Kabir S.M.L., 2009. The role of probiotics in the poultry industry. Int. J. Mol. Sci. 10, 3531–3546, https://doi.org/10.3390/ijms10....
Kayode R.M.O., Sani A., Apata D.F., Joseph J.K., Annongu A.A., Kolawole O.M., Awe S., Obalowu M.A., Arekemase M.A., 2012. Performance and carcass characteristics of broiler chickens fed on fungal mixed-culture (Aspergillus niger and Penicillium chrysogenum) fermented mango kernel cake. Global Res. J. Microbiol. 2, 67–75.
Kim S.K., Kim T.H., Lee S.K., Chang K.H., Cho S.J., Lee K.W., An B.K., 2016. The use of fermented soybean meals during early phase affects subsequent growth and physiological response in broiler chicks. Asian-Australas. J. Anim. Sci. 29, 1287–1293, https://doi.org/10.5713/ajas.1....
Li C.-Y., Lu J.-J., Wu C.-P., Lien T.-F., 2014. Effects of probiotics and bremelain fermented soybean meal replacing fish meal on growth performance, nutrient retention and carcass traits of broilers. Livest. Sci. 163, 94–101, https://doi.org/10.1016/j.livs....
Mathivanan R., Selvaraj P., Nanjappan K., 2006. Feeding of fermented soybean meal on broiler performance. Int. J. Poult. Sci. 5, 868–872, https://doi.org/10.3923/ijps.2....
Missotten J.A., Michiels J., Dierick N., Ovyn A., Akbarian A., De Smet S., 2013. Effect of fermented moist feed on performance, gut bacteria and gut histo-morphology in broilers. Br. Poult. Sci. 54, 627–634, https://doi.org/10.1080/000716....
Nava G.M., Attene-Ramos M.S., Gaskins H.R., Richards J.D., 2009. Molecular analysis of microbial community structure in the chicken ileum following organic acid supplementation. Vet. Microbiol. 137, 345–353, https://doi.org/10.1016/j.vetm....
Nie C., Zhang W., Ge W., Wang Y., Liu Y., Liu J., 2015. Effects of fermented cottonseed meal on the growth performance, apparent digestibility, carcass traits, and meat composition in yellow-feathered broilers. Turk. J. Vet. Anim. Sci. 39, 350–356, https://doi.org/10.3906/vet-14....
PN-ISO 4832, 2007. Horizontal method for determining the number of coliforms - Plate method (in Polish).
PN-EN ISO 7218, 2008. Food and feed microbiology - General requirements and principles of microbiological tests (in Polish).
Savvidou S.E., Beal J.D., Brooks P.H., La Ragione R.M., 2009. Liquid feed fermented with Lactobacillus salivarius reduces susceptibility of broiler chickens to Salmonella enterica typhimurium Sal 1344 nalr. Proc. Br. Soc. Anim. Sci. 2009, 201, https://doi.org/10.1017/S17527....
Seifi K., Karimi Torshizi M.A., Rahimi S., Kazemifard M., 2017. Efficiency of early, single-dose probiotic administration methods on performance, small intestinal morphology, blood biochemistry, and immune response of Japanese quail. Poult. Sci. 96, 2151–2158, https://doi.org/10.3382/ps/pew....
Seo S.-H., Cho S.-J., 2016. Changes in allergenic and antinutritional protein profiles of soybean meal during solid-state fermentation with Bacillus subtilis. LWT – Food Sci. Technol. 70, 208–212, https://doi.org/10.1016/j.lwt.....
Siwicki A.K., Anderson D.P., 1993. Nonspecific defense mechanisms assay in fish. II. Potential killing activity of neutrophils and macrophages, lysozyme activity in serum and organs, and total immunoglobulin (Ig) level in serum. In: A.K. Siwicki, D.P. Anderson, J. Waluga. Fish Diseases Diagnosis and Prevention Methods. The Stanisław Sakowicz Inland Fisheries Institute. Olsztyn (Poland), pp. 105–111.
Smulikowska S., Rutkowski A., 2005. Nutrient Requirements of Poultry. Feeding Recommendations and Nutritive Value of Feed. 4th Edition (in Polish). The Kielanowski Institute of Animal Physiology and Nutrition of the Polish Academy of Sciences, Jabłonna (Poland).
Sugiharto S., Ranjitkar S., 2019. Recent advances in fermented feeds towards improved broiler chicken performance, gastrointestinal tract microecology and immune responses: A review. Anim. Nutr. 5, 1–10, https://doi.org/10.1016/j.anin....
Sun H., Tang J.-w., Yao X.-h., Wu Y.-f., Wang X., Feng J., 2013. Effects of dietary inclusion of fermented cottonseed meal on growth, cecal microbial population, small intestinal morphology, and digestive enzyme activity of broilers. Trop. Anim. Health Prod. 45, 987–993, https://doi.org/10.1007/s11250....
Sunderman F.W. Jr., Nomoto S., 1970. Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clin. Chem. 16, 903–910.
Teng D., Gao M., Yang Y., Liu B., Tian Z., Wang J., 2012. Bio-modification of soybean meal with Bacillus subtilis or Aspergillus oryzae. Biocatal. Agric. Biotechnol. 1, 32–38, https://doi.org/10.1016/j.bcab....
Wang L.C., Wen C., Jiang Z.Y., Zhou Y.M., 2012. Evaluation of the partial replacement of high-protein feedstuff with fermented soybean meal in broiler diets. J. Appl. Poult. Res. 21, 849–855, https://doi.org/10.3382/japr.2....
Yuan L., Chang J., Yin Q., Lu M., Di Y., Wang P., Wang Z., Wang E., Lu F., 2017. Fermented soybean meal improves the growth performance, nutrient digestibility, and microbial flora in piglets. Anim. Nutr. 3, 19–24, https://doi.org/10.1016/j.anin....
Zhang X.-j., Lou H., Li J.-l., Xie D.-x., Xu J., Huo C.-m., Cui Y.-s., 2007. A study on benefit of feeding hy-line white egg with fermented feed. Southwest China J. Agric. Sci. 2007, 529–533.
Effects of Fermented Soybean Meal Supplementation on the Growth Performance and Cecal Microbiota Community of Broiler Chickens
Yang Li, Baozhu Guo, Zhengke Wu, Weiwei Wang, Chong Li, Guohua Liu, Huiyi Cai
The Effect of a Diet Containing Extruded Faba Bean Seeds on Growth Performance and Selected Microbial Activity Indices in the Large Intestine of Piglets
Anna Tuśnio, Marcin Barszcz, Marcin Taciak, Ewa Święch, Agnieszka Wójtowicz, Jacek Skomiał
Assessment of Intestinal Immunity and Permeability of Broilers on Partial Replacement Diets of Two-Stage Fermented Soybean Meal by Bacillus velezensis and Lactobacillus brevis ATCC 367
Chia Tsai, Li Lin, Chao Wang, Ching Tsai, Shang Chang, Tzu Lee
Fermentation of Soybean Meal with Lactobacillus acidophilus Allows Greater Inclusion of Vegetable Protein in the Diet and Can Reduce Vibrionacea in the Intestine of the South American Catfish (Rhamdia quelen)
Oliveira de, Natalia Ha, Cunha da, Luiz Cipriani, André Neto, Everton Skoronski, Enric Gisbert, Fabregat Perez
Effect of feeding fermented soybean meal on broiler chickens’ performance: a meta-analysis
Agung Irawan, Adi Ratriyanto, Adib Respati, Niati Ningsih, Rahma Fitriastuti, Wara Suprayogi, Rendi Hadi, Wahyu Setyono, Novi Akhirini, Anuraga Jayanegara
Animal Bioscience
Effects of fermented soybean meal with Bacillus velezensis, Lactobacillus spp. or their combination on broiler performance, gut antioxidant activity and microflora
C. Tsai, L. Lin, C. Wang, C. Tsai, S. Chang, T. Lee
Animal Bioscience
Mechanism of action, benefits, and research gap in fermented soybean meal utilization as a high-quality protein source for livestock and poultry
Modinat Lambo, Haokai Ma, Haosheng Zhang, Peng Song, Hongxiang Mao, Guowen Cui, Baisheng Dai, Yang Li, Yonggen Zhang
Animal Nutrition
Journals System - logo
Scroll to top