ORIGINAL PAPER
Treatment of alfalfa silage with chitosan at different levels
to determine chemical, nutritional, fermentation,
and microbial parameters
1
Aksaray University, Technical Sciences Vocational School, Department of Food Processing/Milk and Production Technology,
Aksaray, Türkiye
2
Erciyes University, Faculty of Agriculture, Department of Animal Science, 38039 Kayseri,Türkiye
Publication date: 2022-03-17
J. Anim. Feed Sci. 2022;31(1):73-80
KEYWORDS
TOPICS
ABSTRACT
Chitosan is a biodegradable, antimicrobial, antifungal polysaccharide,
thus used as an additive in different industries. Effects of chitosan supplementations
on silage quality traits of alfalfa were investigated in the present
study. Chitosan was supplemented into alfalfa silages at four different levels:
0% – CONT group; 0.5% – CHTS0.5 group; 1% – CHTS1.0 group; and 2% –
CHTS2.0 group. Neutral detergent fibre (NDF) and neutral detergent insoluble
crude protein (NDICP) values increase in CHTS 2.0 group (P < 0.05). Lactic
acid and butyric acid contents decreased (P < 0.05), acetic and propionic acid
contents increased with chitosan treatments. On the day of opening the silages,
mould production was not encountered, and yeast production decreased in
chitosan-supplemented groups. Excessive yeast production was encountered,
but mould development was not seen in chitosan-supplemented groups 5 days
after opening the silages. Silage pH values increased with chitosan treatments
(P < 0.05). So, it can be concluded that chitosan negatively influenced fermentation
quality of alfalfa silage, but reduced mould and clostridial development.
ACKNOWLEDGEMENTS
This study was supported by Scientific Research
Projects Department of Aksaray University (with
the project number: 2019/027). The authors would
like to thank to Dr. Murat Kaya for his valuable suggestions
during the experiments.
CONFLICT OF INTEREST
The Authors declare that there is no conflict of
interest.
METADATA IN OTHER LANGUAGES:
Chinese
不同浓度壳聚糖处理对苜蓿青贮化学、营养、发酵和微生
物参数的影响
关键词：黄曲霉毒素；苜蓿青贮；壳聚糖；产气；微生物；青贮发酵
摘要：壳聚糖是一种可生物降解、抗菌、抗真菌的多糖，因此在不同行业中用作添加剂。本实验研究了添
加壳聚糖对青贮苜蓿品质性状的影响。在苜蓿青贮饲料中添加四种不同水平的壳聚糖：0% - CONT组；0.5%
- CHTS 0.5组；1% - CHTS 1.0组；2% -CHTS 2.0组。CHTS 2.0组的中性洗涤剂纤维（NDF）和中性洗涤剂不溶性
粗蛋白（NDICP）显著升高（P < 0.05）。壳聚糖处理使乳酸和丁酸含量降低（P < 0.05），乙酸和丙酸含量
增加。在打开青贮饲料当天，无霉菌产生，添加壳聚糖组的酵母菌减少。在打开青贮饲料5天后，壳聚糖添
加组中的酵母产量过多，但没有发现霉菌生长。青贮饲料pH值随壳聚糖处理的增加而增加（P < 0.05）。因
此，结论是，壳聚糖对苜蓿青贮发酵质量有负面影响，但会减少霉菌和梭菌的产生。
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