CC-BY 4.0

The effect of lingonberry leaves and oak cortex addition to sheep diets on pancreatic enzymes activity

M. P. Majewska 1  ,  
J. J. Pająk 1,  
J. Skomiał 1,  
R. Miltko 1,  
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
J. Anim. Feed Sci. 2017;26(4):354–358
Publish date: 2017-11-13
Tannins are water-soluble polyphenols with anti-nutritional properties but when supplemented at a low dose to the diets may exhibit positive effect on ruminants. Owing to great group diversity, tannins demonstrate various biological activities. They form complexes with nutrients and so they are enable to affect digestion processes. Thus, the aim of the study was to examine the effect of two types of tannins on sheep pancreatic enzymes activity. In the experiment 6 ewe of the Coloured Merino breed, fitted with catheters to the common pancreatic-biliary duct and simple cannula to the duodenum were used. Sheep were divided into 3 feeding groups for crossover design (n = 2), and fed control diet (CON) and two experimental diets with dried lingonberry leaves (VVI) or oak cortex (QUE) in a dose of 3 g ∙ d–1, as a source of condensed and hydrolysable tannins, respectively. Non-significant effect of tannins on pancreatic-biliary juice secretion and its protein concentration was observed. In sheep fed diets with QUE addition lipase activity was increased in comparison to animals receiving VVI. An upward trend in amylase and trypsin activity after tannins addition was also found. Enriching sheep diets with additives containing tannins slightly stimulated pancreas exocrine activity. However, the inhibitory effects of tannins on endogenous protein activity, similar to the digestive enzymes inhibitors action, cannot be excluded. So, further studies on precise defining the mechanism of tannins action on enzymes activity and digestion process in ruminants are necessary.
M. P. Majewska   
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
1. Ahmed A.E., Smithard R., Ellis M., 1991. Activities of enzymes of the pancreas, and the lumen and mucosa of the small intestine in growing broiler cockerels fed on tannin-containing diets. Br. J. Nutr. 65, 189–197, https://doi.org/10.1079/BJN19910080.
2. AOAC International, 2011. Official Methods of Analysis of AOAC International. 18th Edition. 4th Revision. Gaithersburg, MD (USA).
3. Croom W.J. Jr., Bull L.S., Taylor I.L., 1992. Regulation of pancreatic exocrine secretion in ruminants: a review. J. Nutr. 122, 191–202.
4. Fushiki T., Iwai K., 1989. Two hypotheses on the feedback regulation of pancreatic enzyme secretion. Faseb J. 3, 121–126.
5. Gonçalves R., Soares S., Mateus N., de Freitas V., 2007. Inhibiton of trypsin by condensed tannins and wine. J. Agric. Food Chem. 55, 7596–7601, https://doi.org/10.1021/jf071490i.
6. Grela E.R., Kiczorowska B., Samolińska W., Matras J., Kiczorowski P., Rybiński W., Hanczakowska E., 2017. Chemical composition of leguminous seeds: part I – content of basic nutrients, amino acids, phytochemical compounds, and antioxidant activity. Eur. Food Res. Technol. 243, 1385–1395, https://doi.org/10.1007/s00217-017-2849-7.
7. Grela E.R., Rybiński W., Klebaniuk R., Matras J., 2010. Morphological characteristics of some accessions of grass pea (Lathyrus sativus L.) grown in Europe and nutritional traits of their seeds. Genet. Resour. Crop Evol. 57, 693–701, https://doi.org/10.1007/s10722-009-9505-4.
8. Guilloteau P., Vitari F., Metzinger-Le Meuth V., Le Normand L., Romé V., Savary G., Delaby L., Domeneghini C., Morisset J., 2012. Is there adaptation of the exocrine pancreas in wild animal? The case of the Roe Deer. BMC Vet. Res. 8, 70, https://doi.org/10.1186/1746-6148-8-70.
9. Harmon D.L., 1993. Nutritional regulation of postruminal digestive enzymes in ruminants. J. Dairy Sci. 76, 2102–2111, https://doi.org/10.3168/jds.S0022-0302(93)77545-1.
10. Hummel B.C.W., 1959. A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Can. J. Biochem. Physiol. 37, 1393–1399, https://doi.org/10.1139/o59-157.
11. IZ PIB-INRA, 2009. Standards for Ruminant Feeding (in Polish). National Research Institute of Animal Production. Kraków (Poland).
12. Kapica M., Valverde Piedra J.L., Puzio I., Bieńko M., Szymańczyk S.E., Radzki R.P., 2005. The effect of feeding raw and extruded feed containing soyabean meal on pancreatic juice digestive enzyme activities in young calves. J. Anim. Feed Sci. 14, Suppl. 1, 263–266, https://doi.org/10.22358/jafs/70534/2005.
13. Kowalik B., Kowalczyk J., Pająk J.J., Żebrowska T., Długołęcka Z., 2001. The influence of dietary starch level and type on the activity of pancreatic digestive enzymes in sheep. J. Anim. Feed Sci. 10, Suppl. 2, 57–63, https://doi.org/10.22358/jafs/70034/2001.
14. Kuhla S., Ebmeier C., 1981. Investigations of the tannin content in horse beans. I. Methods for determining condensed tannins in horse beans (in German). Arch. Tierernähr. 31, 573–588, https://doi.org/10.1080/17450398109426870.
15. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.
16. Miao M., Jiang H., Jiang B., Li Y., Cui S.W., Zhang T., 2014. Structure elucidation of catechins for modulation of starch digestion. LWT – Food Sci. Technol. 57, 188–193, https://doi.org/10.1016/j.lwt.2014.01.005.
17. Mueller-Harvey I., 2006. Unravelling the conundrum of tannins in animal nutrition and health. J. Sci. Food Agric. 86, 2010–2037,https://doi.org/10.1002/jsfa.2577.
18. Schofield P., Mbugua D.M., Pell A.N., 2001. Analysis of condensed tannins: a review. Anim. Feed Sci. Technol. 91, 21–40, https://doi.org/10.1016/S0377-8401(01)00228-0.
19. Silanikove N., Landau S., Or D., Kababya D., Bruckental I., Nitsan Z., 2006. Analytical approach and effects of condensed tannins in carob pods (Ceratonia siliqua) on feed intake, digestive and metabolic responses of kids. Livest. Sci. 99, 29–38, https://doi.org/10.1016/j.livprodsci.2005.05.018.
20. Soltan Y.A., Morsy A.S., Sallam S.M.A., Louvandini H., Abdalla A.L., 2012. Comparative in vitro evaluation of forage legumes (prosopis, acacia, atriplex, and leucaena) on ruminal fermentation and methanogenesis. J. Anim. Feed Sci. 21, 759–772, https://doi.org/10.22358/jafs/66148/2012.
21. Waghorn G.C., Shelton I.D., McNabb W.C., McCutcheon S.N., 1994. Effects of condensed tannins in Lotus pedunculatus on its nutritive value for sheep. 2. Nitrogenous aspects. J. Agric. Sci. 123, 109–119, https://doi.org/10.1017/S0021859600067836.
22. Walker J.A., Harmon D.L., 1996. Technical note: A simple, rapid assay for α-amylase in bovine pancreatic juice. J. Anim. Sci. 74, 658–662, https://doi.org/10.2527/1996.743658x.
23. Wang S., Dong S., Zhang R., Shao H., Liu Y., 2014. Effects of proanthocyanidins on porcine pancreatic lipase: Conformation, activity, kinetics and thermodynamics. Process Biochem. 49, 237–243, https://doi.org/10.1016/j.procbio.2013.10.018.
24. Żebrowska T., Kowalczyk J., Pająk J.J., Długołęcka Z., 2001. The influence of dietary protein and fibre on bile-pancreatic secretion in sheep. J. Anim. Feed Sci. 10, 619–626, https://doi.org/10.22358/jafs/68013/2001.