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Effect of diets varying in the type of dietary fibre and its combination with polyphenols on gut function, microbial activity and antioxidant status in rats

E. Żary-Sikorska 1  ,  
A. Jundziłł 3,  
University of Science and Technology,Department of Microbiology and Food Technology, Faculty of Agriculture and Biotechnology, Kordeckiego 20A, 85-225 Bydgoszcz, Poland
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn Poland
Nicolaus Copernicus University, Collegium Medicum, Department of Regenerative Medicine, Karłowicza 24, 85-092 Bydgoszcz, Poland
Nicolaus Copernicus University, Collegium Medicum, Department and Clinic of Geriatrics, M. Curie Skłodowskiej 9, 85-094 Bydgoszcz, Poland
J. Anim. Feed Sci. 2016;25(3):250–258
Publish date: 2016-08-25
The aim of the study was to assess the effects of dietary supplementation with chicory root meal (CHIC) or apple pomace (POM), containing both dietary fibre and polyphenols, on selected physiological parameters in rats. Forty Wistar rats were divided into 5 groups of 8 animals each and fed control (C) diet with 10% of cellulose or diets containing CHIC, POM, oligofructose (OLIGOF) or pectin (PEC) for 28 days. The supplements were added to experimental groups in order to obtain the corresponding to the C group the non-digestible carbohydrates content. Indices of caecal fermentation, antioxidant status, lipid peroxidation and lipid profile of rats were assessed. Supplementing POM and CHIC to rat diets decreased (P ≤ 0.05) the caecal pH and ammonia concentration, bacterial β-glucuronidase activity and increased the short-chain fatty acid concentration and pool size in comparison to the C group. In POM group the triacylglycerol (TAG) and thiobarbituric acid-reactive substances concentration was lower in comparison to C and to OLIGOF and C groups, respectively. The examined preparations significantly (P ≤ 0.05) decreased TAG and total cholesterol levels in blood, but had no influence on liver functioning parameters and antioxidant biomarkers such as sodium dismutase and glutathione peroxidase activity or antioxidant capacity of water- and lipid-soluble serum fractions. So, supplementing rats with POM and CHIC beneficially modulated microbial activity in the caecum and blood lipid profile.
E. Żary-Sikorska   
University of Science and Technology,Department of Microbiology and Food Technology, Faculty of Agriculture and Biotechnology, Kordeckiego 20A, 85-225 Bydgoszcz, Poland
1. Adam C.L., Williams P.A., Garden K.E., Thomson L.M., Ross A.W., 2015. Dose-dependent effects of a soluble dietary fibre (pectin) on food intake, adiposity, gut hypertrophy and gut satiety hormone secretion in rats. PLoS ONE, 10, e0115438, doi:10.1371/ journal.pone.0115438
2. AOAC International, 2005. Official Methods of Analysis of AOAC International. 18th Edition. Gaithersburg, MD (USA)
3. Aprikian O., Duclos V., Guyot S., Besson C., Manach C., Bernalier A., Morand C., Rémésy C., Demingé C., 2003. Apple pectin and a polyphenol-rich apple concentrate are more effective together than separately on caecal fermentation and plasma lipids in rats. J. Nutr. 133, 1860–1865
4. Bouhnik Y., Raskine L., Champion K., Andrieux C., Penven S., Jacobs H., Simoneau G., 2007. Prolonged administration of low-dose inulin stimulates the growth of bifidobacteria in humans. Nutr. Res. 27, 187–193
5. Conceição de Oliviera M., Sichieri R., Moura A.S., 2003. Weight loss associated with a daily intake of three apples or three pears among overweight women. Nutrition 19, 253–256
6. Dahlqvist A., 1964. Method for assay of intestinal disaccharidases. Anal. Biochem. 7, 18–25
7. Fotschki B., Jurgoński A., Juśkiewicz J., Kołodziejczyk K., Sójka M., 2014. Effects of dietary addition of a low-pectin apple preparation on rats. Pol. J. Food Nutr. Sci. 64, 193–199
8. Hambly R.J., Rumney C.J., Cunninghame M., Fletcher J.M.E., Rijken P.J., Rowland I.R., 1997. Influence of diets containing high and low risk factors for colon cancer on early stages of carcinogenesis in human-flora-associated (HFA) rats. Carcinogenesis 18, 1535–1539
9. Han K.-H., Kobayashi Y., Nakamura Y., Shimada K., Aritsuka T., Ohba K., Morita T., Fukushima M., 2014. Comparison of the effects of longer chain inulins with different degrees of polymerization on colonic fermentation in a mixed culture of swine fecal bacteria. J. Nutr. Sci. Vitaminol. 60, 206–212
10. Hippe B., Remely M., Aumueller E., Pointner A., Haslberg A.G., 2015. SCFA producing gut microbiota and its effects on the epigenetic regulation of inflammation. In: M.-T. Liong (Editor). Beneficial Microorganisms in Medical and Health Applications. Microbiology Monographs. Vol. 28. Springer International Publishing AG. Cham (Switzerland), pp. 181–197
11. Hong M.Y., Turner N.D., Murphy M.E., Carroll R.J., Chapkin R.S., Lupton J.R., 2015. In vivo regulation of colonic cell proliferation, differentiation, apoptosis and P27Kip1 by dietary fish oil and butyrate in rats. Cancer Prev. Res. 8, 1076–1083
12. Juśkiewicz J., Zduńczyk Z., Jurgoński A., Brzuzan Ł., Godycka-Kłos I., Żary-Sikorska E., 2008. Extract of green tea leaves partially attenuates streptozotocin-induced changes in antioxidant status and gastrointestinal functioning in rats. Nutr. Res. 28, 343–349
13. Juśkiewicz J., Zduńczyk Z., Żary-Sikorska E., Król B., Milala J., Jurgoński A., 2011. Effect of the dietary polyphenolic fraction of chicory root, peel, seed and leaf extracts on caecal fermentation and blood parameters in rats fed diets containing prebiotic fructans. Brit. J. Nutr. 105, 710–720
14. Juśkiewicz J., Żary-Sikorska E., Zduńczyk Z., Król B., Jarosławska J., Jurgoński A., 2012. Effect of dietary supplementation with unprocessed and ethanol-extracted apple pomaces on caecal fermentation, antioxidant and blood biomarkers in rats. Brit. J. Nutr. 107, 1138–1146
15. Koruda M.J., Rolandelli R.H., Settle R.G., Rombeau J.L., 1988. Small bowel disacchridase activity in the rat as affected by intestinal resection and pectin feeding. Am. J. Clin. Nutr. 47, 448–453
16. Louis P., Scott K.P., Duncan S.H., Flint H.J., 2007. Understanding the effects of diet on bacterial metabolism in the large intestine. J. Appl. Microbiol. 102, 1197–1208
17. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275
18. Milala J., Grzelak K., Król B., Juśkiewicz J., Zduńczyk Z., 2009. Composition and properties of chicory extracts rich in fructans and polyphenols. Pol. J. Food Nutr. Sci. 59, 35–43
19. Olthof M.R., Hollman P.C.H., Katan M.B., 2001. Chlorogenic acid and caffeic acid are absorbed in humans. J. Nutr. 131, 66–71
20. Pirman T., Ribeyre M.C., Mosoni L., Rémond D., Vrecl M., Salobir J., Mirand P.P., 2007. Dietary pectin stimulates protein metabolism in the digestive tract. Nutrition 23, 69–75
21. Schwartz S.E., Starr C., Bachman S., Holtzapple P.G., 1983. Dietary fiber decreases cholesterol and phospholipid synthesis in rat intestine. J. Lipid Res. 24, 746–752
22. Taciak M., Barszcz M., Tuśnio A., Pastuszewska B., 2015. Interactive effects of indigestible carbohydrates, protein type, and protein level on biomarkers of large intestine health in rats. PLoS ONE 10, e0142176, doi:10.1371/journal.pone.0142176
23. Topping D.L., Clifton P.M., 2001. Short-chain fatty acids and human colonic function: Roles of resistant starch and nonstarch polysaccharides. Physiol. Rev. 81, 1031–1064
24. Tuohy K.M., Rouzaud G.C.M., Brück W.M., Gibson G.R., 2005. Modulation of the human gut microflora towards improved health using prebiotics – assessment of efficacy. Curr. Pharm. Design 11, 75–90
25. Uchiyama M., Mihara M., 1978. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem. 86, 271–278
26. Wolfe K.L., Liu R.H., 2003. Apple peels as value-added food ingredient. J. Agr. Food Chem. 51, 1676–1683
27. Wronkowska M., Juśkiewicz J., Zduńczyk Z., Soral-Śmietana M., Krupa-Kozak U., 2011. Influence of chemically-modified potato starch (RS type 4) on the nutritional and physiological indices of rats. Pol. J. Food Nutr. Sci. 61, 143–151
28. Zduńczyk Z., Juśkiewicz J., Estrella I., 2006. Cecal parameters of rats fed diets containing grapefruit polyphenols and inulin as single supplements or in a combination. Nutrition 22, 898–904
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