A highly efficient method for determination of
allantoin in ruminal digesta, ovine urine and
blood serum by HPLC

A sensitive HPLC method for direct determination of allantoin in ruminal digesta, urine and blood serum of sheep is described. Blood serum and urine samples were diluted 1:3 with deionized water. Separation and quantification of allantoin was achieved using three long Nova Pac C₁₈-columns (Waters) and UV detection at 205 and 215 nm. The Iow coefficient of variation and satisfactory recovery of allantoin added to assayed samples (~100%) provide a suitable tool for routine quantification of allantoin in a large number of urine, ruminal digesta and blood serum samples.

CITATIONS (1):

Simultaneous determination of chondroitin sulfate sodium, allantoin and pyridoxine hydrochloride in pharmaceutical eye drops by an ion-pair high-performance liquid chromatography
Pengfei Jin, Jie Ma, Xuejun Wu, Ding Zou, Chunhua Sun, Xin Hu
Journal of Pharmaceutical and Biomedical Analysis

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