0.917
IF5
1.024
IF
Q2
JCR
0.90
CiteScore
0.385
SJR
Q2
SJR
20
MNiSW
142.18
ICV
SHORT COMMUNICATION
 
CC-BY 4.0
 
 

A highly efficient method for determination of allantoin in ruminal digesta, ovine urine and blood serum by HPLC

M. Czauderna 1  ,  
J. Kowalczyk 1,  
 
1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
J. Anim. Feed Sci. 2004;13(Suppl. 2):67–70
Publish date: 2004-09-16
KEYWORDS:
ABSTRACT:
A sensitive HPLC method for direct determination of allantoin in ruminal digesta, urine and blood serum of sheep is described. Blood serum and urine samples were diluted 1:3 with deionized water. Separation and quantification of allantoin was achieved using three long Nova Pac C18-columns (Waters) and UV detection at 205 and 215 nm. The Iow coefficient of variation and satisfactory recovery of allantoin added to assayed samples (~100%) provide a suitable tool for routine quantification of allantoin in a large number of urine, ruminal digesta and blood serum samples.
CORRESPONDING AUTHOR:
M. Czauderna   
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
ISSN:1230-1388