ORIGINAL PAPER
Changes in nuclear factor kappa B components expression in the ovine spleen during early pregnancy
1
Hebei University of Engineering, School of Life Sciences and Food Engineering, No. 19 Taiji Road, Handan 056038, China
Publication date: 2022-02-27
J. Anim. Feed Sci. 2022;31(1):3-11
KEYWORDS
TOPICS
ABSTRACT
Normal pregnancy is characterised by a systemic immunological
tolerance against foetal antigens, and the spleen contributes to the adaptive
immune tolerance during pregnancy. Nuclear factor kappa B (NF-κB)
signallings participate in splenic immune regulation, but it is unclear whether
there are changes in NF-κB components expression in the ovine spleen
during early pregnancy. The objective of this study was to explore the effects
of early pregnancy on the expression of NF-κB components in the maternal
spleen in sheep. The spleens were sampled on day 16 of the oestrous
cycle, and on days 13, 16 and 25 of gestation. The expression of NF-κB
components, including NF-κB1 (p50), NF-κB2 (p52), RelA (p65), RelB and
c-Rel, were detected by quantitative real-time PCR, Western blot analysis and
immunohistochemical analysis. The results showed that NF-κB1 and RelB
mRNA and proteins decreased at days 13 and 16 of pregnancy, but increased
at day 25 of pregnancy in comparison with that on day 16 of the oestrous
cycle. Nevertheless, NF-κB2 and RelA mRNA and proteins peaked at days
13 and 16 of pregnancy. In addition, early pregnancy inhibited C-Rel expression
at days 16 to 25 of pregnancy in the maternal spleen. In conclusion, the variable
expression of individual NF-κB components was found in the ovine spleen
during early pregnancy, which may be related with pregnancy recognition, and
essential for the embryo implantation and pregnancy maintenance.
ACKNOWLEDGEMENTS
The current study was supported by the grants
from Natural Science Foundation of Hebei Province,
China (C2021402019), and Hebei Science and
Technology Bureau, China (21326601D).
CONFLICT OF INTEREST
The authors declare that there is no conflict of
interest.
METADATA IN OTHER LANGUAGES:
Chinese
早期妊娠对绵羊脾脏表达核因子-κB组分的影响
摘要
核因子-κB组分, 妊娠, 绵羊, 脾脏
正常妊娠情况下，母体对胎儿抗原产生系统性免疫耐受。在妊娠期间，脾脏参与母体适应性免疫耐受
的形成。核因子-κB (Nuclear factor kappa B, NF-κB) 信号参与脾脏的免疫调节，但是，妊娠早期绵羊脾脏表达
NF-κB组分是否发生改变，目前还不清楚。本研究的目的是探讨早期妊娠对绵羊母体脾脏表达NF-κB组分的
影响。采集发情周期第16天，以及妊娠第13、16和25天的母羊脾脏，采用实时定量PCR、蛋白印迹和免疫
组化分析NF-κB组分的表达，NF-κB组分包括NF-κB1 (p50)、NF-κB2 (p52)、RelA (p65)、RelB和C-Rel。试验结
果表明，与发情周期第16天相比，在妊娠第13天和16天，脾脏表达NF-κB1和 RelB的mRNA和蛋白质减少，
但是，在妊娠第25天却增加。此外，在妊娠第13天和16天，NF-κB2和RelA的mRNA和蛋白质水平达到峰值。
同时，从妊娠第16至25天，母体脾脏C-Rel的表达水平逐步降低。综上所述，在绵羊妊娠早期，脾脏表达
NF-κB不同组分发生改变，这可能与妊娠识别有关，对胚胎附植和妊娠维持至关重要。
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