ORIGINAL PAPER
Brain-derived neurotrophic factor (BDNF) affects somatotrophic
axis activity in sheep
1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
Publication date: 2021-12-13
Corresponding author
A. Wójcik-Gładysz
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
J. Anim. Feed Sci. 2021;30(4):329-339
KEYWORDS
TOPICS
ABSTRACT
Brain-derived neurotrophic factor (BDNF) is a growth protein factor
belonging to the neurotrophin peptide family that is involved in the regulation
of several activities of the central nervous system. However, its effects on the
activity of the somatotrophic axis in ruminants have not yet been determined.
The present study aimed to verify the research hypothesis, which assumed
that BDNF can modulate key hormones of the somatotrophic axis in sheep.
The experiment involved sexually mature Polish Merino sheep (n = 24). The
animals were divided into three groups, and the following intracerebroventricular
infusions were performed: control group received Ringer-Locke solution (480
μl/day), BDNF10 – BDNF at a dose of 10 μg/480 μl/day and BDNF60 – BDNF
at a dose of 60 μg/480 μl/day. Blood samples were collected on days 0 and 3
of infusions. After the experiment, the animals were slaughtered and selected
structures of the hypothalamus, pituitaries and plasma samples were stored
for Real-Time reverse transcription-polymerase chain reaction (Real-Time
RT-PCR) and radioimmunoassay analysis. It was revealed that central BDNF
administration induced a dose-dependent increase in growth hormone relasing
hormone (GHRH) mRNA expression in the mediobasal hypothalamus of sheep.
Moreover, central BDNF administration increased growth hormone (GH) mRNA
expression in the pituitary and the GH pulse amplitude, which, consequently,
increased the mean GH concentration in the blood plasma. These observations
suggest the up-regulation of the somatotrophic axis activity in female sheep after
BDNF infusion. So, it can be stated that BDNF is involved in the modulation of
key hormones co-creating the somatotrophic axis in mature sheep.
ACKNOWLEDGEMENTS
The authors would like to thank veterinary surgeons K. Roszkowicz-Ostrowska and J. Rutkowski for the help in brain surgery, E. Skrzeczyńska, A. Misztal and K. Biernacka for the help in performing the experiments, and W. Mrozek and R. Druchniak for animal care. Presented study is the part of B.J. Przybył PhD dissertation.
FUNDING
This research was supported by the funds provided by the National Science Centre (Poland) PRELUDIUM 9 grant no. 2015/17/N/NZ9/01110.
CONFLICT OF INTEREST
The authors declared that there is no conflict of interest.
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