1.054
IF5
1.150
IF
Q3
JCR
1.7
CiteScore
0.396
SJR
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SJR
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MNiSW
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ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

The effects of benzoic acid and essential oil compounds in combination with protease on the performance of chickens

I. A. Giannenas 1  ,  
E. Tsalie 3,  
D. Tontis 3,  
 
1
Aristotle University of Thessaloniki, School of Veterinary Medicine, Laboratory of Nutrition, 54124 Thessaloniki, Greece
2
University of Thessaly, Veterinary Faculty, Laboratory of Biochemistry, 43100 Karditsa, Greece
3
University of Thessaly, Veterinary Faculty, Laboratory of Pathology, 43100 Karditsa, Greece
4
Military Veterinary Training and Nursing Centre, 41334 Larisa, Thessaly, Greece
J. Anim. Feed Sci. 2014;23(1):73–81
Publication date: 2014-03-03
KEYWORDS
ABSTRACT
Experiments were conducted to study the effect of benzoic acid and of essential oil blends in combination with protease on the growth performance of broiler chickens. In the first trial, the birds were divided into three dietary treatments. The control group was fed a basal diet, while the other two groups were given benzoic acid at 300 and 1000 mg · kg–1, respectively. Growth performance was not affected by benzoic acid inclusion. The pH values of the caecal content decreased following benzoic acid supplementation, while no differences were noticed in the pH of the crop, gizzard, ileum and rectum contents. Following benzoic acid supplementation, lactic acid bacteria populations increased in the caecum, and coliform bacteria, decreased. In the second trial, the birds were divided into three dietary treatments. The controls were fed a basal diet, while the other two groups were given thymol and a mixture of essential oil compounds (30 mg · kg–1). The dietary inclusion of the mixture of essential oil compounds enhanced growth performance compared with the other groups (P < 0.05), increased lactic acid bacteria populations, and decreased the coliform bacteria population in the caecum. In the third trial, the control group was fed the basal diet, while the other group was given a diet with similar ingredients and containing more benzoic acid and a mixture of essential oils, protease, and less protein and amino acids. In vitro tests showed that addition of benzoic acid, the mixture of essential oils and protease reduced buffering capacity compared with control feed and simulation experiments revealed that the protease increased protein extraction, hydrolysis and digestion. The combination of benzoic acid, essential oils and protease effectively improved weight gain and the feed conversion ratio compared with the control, as well as villus height, lactic acid bacteria counts, and reduced coliform bacteria counts compared with the control group. Finally, it was demonstrated for the first time that the novel, acid-stable protease increases protein solubilization, hydrolysis and digestion in an in vitro simulation model.
CORRESPONDING AUTHOR
I. A. Giannenas   
Aristotle University of Thessaloniki, School of Veterinary Medicine, Laboratory of Nutrition, 54124 Thessaloniki, Greece
 
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L.J. BROOM
World's Poultry Science Journal
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The Journal of Applied Poultry Research
3. Effects of Oregano, Attapulgite, Benzoic Acid and their Blend on Chicken Performance, Intestinal Microbiology and Intestinal Morphology
Athina Tzora, Ilias Giannenas, Achilleas Karamoutsios, Nikolaos Papaioannou, Dimitrios Papanastasiou, Eleftherios Bonos, Stylianos Skoufos, Thomas Bartzanas, Ioannis Skoufos
The Journal of Poultry Science
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Subramaniam Mohana Devi, Kwang Yong Lee, In Ho Kim
Revista Brasileira de Zootecnia
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Poultry Science
6. Effects of Protease Addition and Replacement of Soybean Meal by Corn Gluten Meal on the Growth of Broilers and on the Environmental Performances of a Broiler Production System in Greece
Ilias Giannenas, Eleftherios Bonos, Vasileios Anestis, Georgios Filioussis, Dimitrios K. Papanastasiou, Thomas Bartzanas, Nikolaos Papaioannou, Athina Tzora, Ioannis Skoufos, Gotthard Kunze
PLOS ONE
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I. Giannenas, D. Doukas, A. Karamoutsios, A. Tzora, E. Bonos, I. Skoufos, A. Tsinas, E. Christaki, D. Tontis, P. Florou-Paneri
Animal Feed Science and Technology
8. Effect of Different Dietary Levels of Rosemary (Rosmarinus Officinalis) and Yarrow (Achillea Millefolium) on the Growth Performance, Carcass Traits and Ileal Micro-biota of Broilers
Behzad Norouzi, Ali Ahmad Alaw Qotbi, Alireza Seidavi, Achille Schiavone, Andrés L. Martínez Marín
Italian Journal of Animal Science
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S A Lee, M R Bedford, C L Walk
Poultry Science
10. Enzymes and/or combination of organic acid and essential oils supplementation in pasture-fed free-range laying hens increased the digestibility of nutrients and non-starch polysaccharides
Zafar Iqbal, Florian Metzger, Mini Singh, Natalie Morgan, Robert A Swick, Rider A Perez-Maldonado, Shawkat A M'Sadeq, Jürgen Zentek, Isabelle Ruhnke
Poultry Science
11. Feed Additives
Ch Franz, K.H.C. Baser, I. Hahn-Ramssl
12. Feed Additives
Li-Zhi Jin, Yueming Dersjant-Li, Ilias Giannenas
13. Feed Additives
Ioannis Skoufos, Eleftherios Bonos, Ioannis Anastasiou, Anastasios Tsinas, Athina Tzora
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Gayatri Suresh, Hooshang Pakdel, Tarek Rouissi, Satinder Brar, Moussa Diarra, Christian Roy
Brazilian Journal of Microbiology
ISSN:1230-1388