ORIGINAL PAPER
The effects of benzoic acid and essential oil compounds in combination with protease on the performance of chickens
 
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1
Aristotle University of Thessaloniki, School of Veterinary Medicine, Laboratory of Nutrition, 54124 Thessaloniki, Greece
2
University of Thessaly, Veterinary Faculty, Laboratory of Biochemistry, 43100 Karditsa, Greece
3
University of Thessaly, Veterinary Faculty, Laboratory of Pathology, 43100 Karditsa, Greece
4
Military Veterinary Training and Nursing Centre, 41334 Larisa, Thessaly, Greece
CORRESPONDING AUTHOR
I. A. Giannenas   

Aristotle University of Thessaloniki, School of Veterinary Medicine, Laboratory of Nutrition, 54124 Thessaloniki, Greece
Publication date: 2014-03-03
 
J. Anim. Feed Sci. 2014;23(1):73–81
 
KEYWORDS
ABSTRACT
Experiments were conducted to study the effect of benzoic acid and of essential oil blends in combination with protease on the growth performance of broiler chickens. In the first trial, the birds were divided into three dietary treatments. The control group was fed a basal diet, while the other two groups were given benzoic acid at 300 and 1000 mg · kg–1, respectively. Growth performance was not affected by benzoic acid inclusion. The pH values of the caecal content decreased following benzoic acid supplementation, while no differences were noticed in the pH of the crop, gizzard, ileum and rectum contents. Following benzoic acid supplementation, lactic acid bacteria populations increased in the caecum, and coliform bacteria, decreased. In the second trial, the birds were divided into three dietary treatments. The controls were fed a basal diet, while the other two groups were given thymol and a mixture of essential oil compounds (30 mg · kg–1). The dietary inclusion of the mixture of essential oil compounds enhanced growth performance compared with the other groups (P < 0.05), increased lactic acid bacteria populations, and decreased the coliform bacteria population in the caecum. In the third trial, the control group was fed the basal diet, while the other group was given a diet with similar ingredients and containing more benzoic acid and a mixture of essential oils, protease, and less protein and amino acids. In vitro tests showed that addition of benzoic acid, the mixture of essential oils and protease reduced buffering capacity compared with control feed and simulation experiments revealed that the protease increased protein extraction, hydrolysis and digestion. The combination of benzoic acid, essential oils and protease effectively improved weight gain and the feed conversion ratio compared with the control, as well as villus height, lactic acid bacteria counts, and reduced coliform bacteria counts compared with the control group. Finally, it was demonstrated for the first time that the novel, acid-stable protease increases protein solubilization, hydrolysis and digestion in an in vitro simulation model.
 
CITATIONS (20):
1.
Organic acids for improving intestinal health of poultry
L.J. BROOM
World's Poultry Science Journal
 
2.
A blend of benzoic acid and essential oil compounds as an alternative to antibiotic growth promoters in broiler diets
P. C. Aristimunha, A. P. Rosa, L. S. Boemo, D. C. Garcez, D. P. Rosa, A. Londero, A. Scher, J. Forgiarini
The Journal of Applied Poultry Research
 
3.
Effects of Oregano, Attapulgite, Benzoic Acid and their Blend on Chicken Performance, Intestinal Microbiology and Intestinal Morphology
Athina Tzora, Ilias Giannenas, Achilleas Karamoutsios, Nikolaos Papaioannou, Dimitrios Papanastasiou, Eleftherios Bonos, Stylianos Skoufos, Thomas Bartzanas, Ioannis Skoufos
The Journal of Poultry Science
 
4.
Analysis of the effect of dietary protected organic acid blend on lactating sows and their piglets
Subramaniam Mohana Devi, Kwang Yong Lee, In Ho Kim
Revista Brasileira de Zootecnia
 
5.
Effects of commercial organic acid blends on male broilers challenged with E. coli K88: Performance, microbiology, intestinal morphology, and immune response
N. Khodambashi Emami, A. Daneshmand, S. Zafari Naeini, E. N. Graystone, L. J. Broom
Poultry Science
 
6.
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Ilias Giannenas, Eleftherios Bonos, Vasileios Anestis, Georgios Filioussis, Dimitrios K. Papanastasiou, Thomas Bartzanas, Nikolaos Papaioannou, Athina Tzora, Ioannis Skoufos, Gotthard Kunze
PLOS ONE
 
7.
Effects of Enterococcus faecium , mannan oligosaccharide, benzoic acid and their mixture on growth performance, intestinal microbiota, intestinal morphology and blood lymphocyte subpopulations of fattening pigs
I. Giannenas, D. Doukas, A. Karamoutsios, A. Tzora, E. Bonos, I. Skoufos, A. Tsinas, E. Christaki, D. Tontis, P. Florou-Paneri
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Effect of Different Dietary Levels of Rosemary (Rosmarinus Officinalis) and Yarrow (Achillea Millefolium) on the Growth Performance, Carcass Traits and Ileal Micro-biota of Broilers
Behzad Norouzi, Ali Ahmad Alaw Qotbi, Alireza Seidavi, Achille Schiavone, Andrés L. Martínez Marín
Italian Journal of Animal Science
 
9.
Meta-analysis: explicit value of mono-component proteases in monogastric diets
S A Lee, M R Bedford, C L Walk
Poultry Science
 
10.
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Zafar Iqbal, Florian Metzger, Mini Singh, Natalie Morgan, Robert A Swick, Rider A Perez-Maldonado, Shawkat A M'Sadeq, Jürgen Zentek, Isabelle Ruhnke
Poultry Science
 
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Ch Franz, K.H.C. Baser, I. Hahn-Ramssl
 
12.
Feed Additives
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13.
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Gayatri Suresh, Hooshang Pakdel, Tarek Rouissi, Satinder Brar, Moussa Diarra, Christian Roy
Brazilian Journal of Microbiology
 
15.
Effects of phytase and benzoic acid supplementation on growth performance, nutrient digestibility, tibia mineralization and serum traits in male broiler chickens
Mozhdeh Karami, Ahmad Karimi, AmirAli Sadeghi, Jürgen Zentek, Farshad Boroojeni
Livestock Science
 
16.
Effect of benzoic acid on production performance, egg quality, intestinal morphology and cecal microbial community of laying hens
Haojie Gong, Zengqiao Yang, Pietro Celi, Lei Yan, Xuemei Ding, Shiping Bai, Qiufeng Zeng, Shenyu Xu, Zhuowei Su, Yong Zhuo, Keying Zhang, Jianping Wang
Poultry Science
 
17.
Potential Effects of Acidifier and Amylase as Substitutes for Antibiotic on the Growth Performance, Nutrient Digestion and Gut Microbiota in Yellow-Feathered Broilers
Yibing Wang, Yang Wang, Xiajing Lin, Zhongyong Gou, Qiuli Fan, Jinling Ye, Shouqun Jiang
Animals
 
18.
Eubiotic effect of a dietary potassium diformate (KDF) and probiotic (Lactobacillus acidophilus) on growth, hemato-biochemical indices, antioxidant status and intestinal functional topography of cultured Nile tilapia Oreochromis niloticus fed diet free fishmeal
Mohamed Hassaan, A.M.I. El-Sayed, Eman Mohammady, Mohamed Zaki, Mona Elkhyat, S. Jarmołowicz, Ehab El-Haroun
Aquaculture
 
19.
Evaluation of interactive effects of phytase and benzoic acid supplementation on performance, nutrients digestibility, tibia mineralisation, gut morphology and serum traits in male broiler chickens
Mozhdeh Karami, Ahmad Karimi, Amirali Sadeghi, Jürgen Zentek, Boroojeni Goodarzi
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Inhibitory action of Lippia gracilis Schauer essential oil on pathogenic bacteria and its effects as a growth promoter on quail
Gilmar Cardoso-Júnior, Graziele Rocha, Douglas Souza, Claudia Lopes, Hanna Pereira, Arie Blank, Flavio Barbosa, Camilla Silva, Paula Rodrigues, Gregorio Oliveira-Júnior
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ISSN:1230-1388