ORIGINAL PAPER
Modification of ileal proteome in growing pigs by dietary supplementation with inulin or dried chicory root
| 1 | West Pomeranian University of Technology, Department of Physiology, Cytobiology and Proteomics, Klemensa Janickiego 29, 71-270 Szczecin, Poland |
| 2 | The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Department of Animal Nutrition,
Instytucka 3, 05-110 Jabłonna, Poland |
CORRESPONDING AUTHOR
A. Lepczyński
West Pomeranian University of Technology, Department of Physiology, Cytobiology and Proteomics, Klemensa Janickiego 29, 71-270 Szczecin, Poland
West Pomeranian University of Technology, Department of Physiology, Cytobiology and Proteomics, Klemensa Janickiego 29, 71-270 Szczecin, Poland
Publication date: 2019-06-07
J. Anim. Feed Sci. 2019;28(2):177–186
KEYWORDS
TOPICS
ABSTRACT
Recently, numerous plant-based preparations have been used for
health promotion or disease prevention in animals. Chicory is one of the plants
that contain various nutraceutics, mainly inulin type fructans (ITFs) showing
prebiotic character. In pigs, a significant proportion of ITFs is fermented in the
distal part of the small intestine to lactate and short-chain fatty acids (SCFAs).
Previous studies have shown that SCFAs are involved in structural rearrangement of the intestinal epithelial mucosa and stimulate intestinal barrier assembly. These changes should be accompanied by a modification of enterocyte
protein composition and abundance. Thus, we hypothesized that ITFs, due
to their direct or indirect effect, can modify ileal proteome. The experiment
was performed on 24 castrated male pigs (PIC × Penarlan P76) assigned to
3 groups (n = 8) fed cereal-based diets: control or experimental: supplemented
with 4% of dried chicory root or with 2% of inulin. Mucosa proteins were separated using two-dimensional electrophoresis, followed by the identification of
statistically valid proteins with the aid of Matrix Associated Laser Desorption
Ionization – Time of Flight mass spectrometry (MALDI-TOF MS). Experimental
diets significantly altered expression of proteins involved in: glycolysis/gluconeogenesis, biosynthesis of amino acids, cytoskeleton rearrangement, protein
synthesis and processing, cell proliferation and differentiation, and iron absorption. Changes in the expression of proteins associated with energetic metabolism, cell proliferation and cytoskeleton rearrangement may suggest an impact
of dried chicory root on the functional maturation of the ileal mucosa. Additionally, changes in transferrin abundance suggest the significance of chicory root
and inulin supplementation for intestinal iron absorption.
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CITATIONS (3):
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