ORIGINAL PAPER
Proteome changes in ileal mucosa of young pigs resulting
from different levels of native chicory inulin in the diet
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| 1 | West Pomeranian University of Technology, Department of Physiology, Cytobiology and Proteomics
Klemensa Janickiego 29, 71-270 Szczecin, Poland |
| 2 | The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Department of Animal Nutrition, Instytucka 3, 05-110 Jabłonna, Poland |
CORRESPONDING AUTHOR
A. Herosimczyk
West Pomeranian University of Technology, Department of Physiology, Cytobiology and Proteomics Klemensa Janickiego 29, 71-270 Szczecin, Poland
West Pomeranian University of Technology, Department of Physiology, Cytobiology and Proteomics Klemensa Janickiego 29, 71-270 Szczecin, Poland
Publication date: 2018-08-20
J. Anim. Feed Sci. 2018;27(3):229–237
KEYWORDS
TOPICS
gastrointestinal track and digestionpigsprobiotics, prebiotics, symbiotics, eubiotics, antibioticsphysiology
ABSTRACT
The analysis of mucosa proteomes was performed using a twodimensional
electrophoresis combined with mass spectrometry to determine the
effect of dietary level of inulin on protein expression patterns in the ileum. The
experiment was carried out on 24 castrated male piglets, allocated to three groups,
fed from the day 10 of life an unsupplemented diet (C) or diet supplemented with
1% (T1) or 3% (T2) of native chicory inulin. Samples of ileal tissue and blood
were collected after 40 days of feeding. Comparative proteomic analysis of the
T1 group showed 10 protein spots with a decreased expression, whereas the
T2 diet caused overexpression of 24 spots in comparison to the C diet. Inulin
levels differed in their effects on the expression of ileal proteins involved in
intracellular molecular mechanisms controlling cell division and growth. The
T1 diet down-regulated, whereas the T2 diet induced substantial up-regulation
of proteins engaged in transcriptional and translational activities, folding and
posttranslational modifications, which may indicate stimulation of epithelial cell
proliferation. Inulin did not affect plasma levels of phosphorus, magnesium,
calcium and iron in piglets but improved plasma prooxidant-antioxidant balance
in animals fed the T2 diet. The results of this study might be considered as
preliminary since further confirmation using more sophisticated quantitative
proteomic tools is required to better understand and answer the unresolved
issues concerning the mechanism underlying the inulin effect on the ileum in
growing pigs. Nevertheless, a general insight into how inulin molecules or their
fermentation end-products may induce changes in protein expression patterns
in the ileum was presented.
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