Improved derivatization of malondialdehyde with 2-thiobarbituric acid for evaluation of oxidative stress in selected tissues of chickens
More details
Hide details
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
M. Czauderna   

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Publication date: 2014-06-11
J. Anim. Feed Sci. 2014;23(2):190–197
A selective and sensitive method based on derivatization with 2-thiobarbituric acid and ultra-fast liquid chromatographic separation is described for the determination of malondialdehyde (MDA) in chicken liver, muscles and adipose tissue, and in lard and fish oil. Preparation of samples involves acid hydrolysis and derivatization. Separation is achieved using an Accucore C18-column (2.6 μm, Hydro-RP, 150×3.0 mm), an acetonitrile gradient in water, and detection at 530 nm. The results indicate that external calibration based on standard solutions of MDA may be used for measuring the MDA concentration in adipose tissue, lard and fish oil due to the absence of matrix effects. The MDA concentration in protein-rich biological samples should be calculated as the difference between the MDA concentration measured in MDA-spiked and unspiked samples of the same specimen and mass and the known concentration of the MDA spike. For liver or muscle samples, we suggest using external calibration based on standard solutions of MDA added to the same mass of liver or muscles. The proposed method is suitable for rapid and sensitive analysis of MDA in samples of animal origin or in plant oils. The method can also be suitable for routine evaluation of oxidative stress in animal tissues and oxidative stability of biological materials and animal products.
Interactive effects of dietary lipids and vitamin E level on performance, blood eicosanoids, and response to mitogen stimulation in broiler chickens of different ages
P. Konieczka, M. Barszcz, N. Chmielewska, M. Cieślak, M. Szlis, S. Smulikowska
Poultry Science
Beneficial effects of enrichment of chicken meat with n-3 polyunsaturated fatty acids, vitamin E and selenium on health parameters: a study on male rats
P. Konieczka, A. J. Rozbicka-Wieczorek, M. Czauderna, S. Smulikowska
The enrichment of chicken meat with omega-3 fatty acids by dietary fish oil or its mixture with rapeseed or flaxseed—Effect of feeding duration
P. Konieczka, M. Czauderna, S. Smulikowska
Animal Feed Science and Technology
The interactive effect of dietary n-6: n-3 fatty acid ratio and vitamin E level on tissue lipid peroxidation, DNA damage in intestinal epithelial cells, and gut morphology in chickens of different ages
P Konieczka, M Barszcz, M Choct, S Smulikowska
Poultry Science
Redox status, and lipid peroxidation and protein oxidation levels in small ruminants
S. Cecchini, G. Piccione, C. Saoca, G. Giangrosso, A. Caputo, F. Fazio
Journal of Animal and Feed Sciences
Analysis of malondialdehyde in human plasma samples through derivatization with 2,4-dinitrophenylhydrazine by ultrasound-assisted dispersive liquid–liquid microextraction-GC-FID approach
Reyhane Malaei, Amir M. Ramezani, Ghodratollah Absalan
Journal of Chromatography B
Simultaneous determination of malondialdehyde and 3-nitrotyrosine in cultured human hepatoma cells by liquid chromatography-mass spectrometry
Nela Vanova, Lubica Muckova, Monika Schmidt, David Herman, Alzbeta Dlabkova, Jaroslav Pejchal, Daniel Jun
Biomedical Chromatography
Nanocomposite Furcellaran Films—the Influence of Nanofillers on Functional Properties of Furcellaran Films and Effect on Linseed Oil Preservation
Ewelina Jamróz, Pavel Kopel, Joanna Tkaczewska, Dani Dordevic, Simona Jancikova, Piotr Kulawik, Vedran Milosavljevic, Kristyna Dolezelikova, Kristyna Smerkova, Pavel Svec, Vojtech Adam
The effect of fish oil, lycopene and organic selenium as feed additives on rabbit meat quality
Jaworska Danuta, Czauderna Marian, Przybylski Wiesław, Ranachowska Anna
Journal of Applied Animal Research
Osteocalcin prevents insulin resistance, hepatic inflammation, and activates autophagy associated with high-fat diet induced fatty liver hemorrhagic syndrome in aged laying hens
X.L. Wu, X.Y. Zou, M. Zhang, H.Q. Hu, X.L. Wei, M.L. Jin, H.W. Cheng, S. Jiang
Poultry Science