ORIGINAL PAPER
Improved derivatization of malondialdehyde with 2-thiobarbituric acid for evaluation of oxidative stress in selected tissues of chickens
 
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The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
CORRESPONDING AUTHOR
M. Czauderna   

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Publication date: 2014-06-11
 
J. Anim. Feed Sci. 2014;23(2):190–197
 
KEYWORDS
ABSTRACT
A selective and sensitive method based on derivatization with 2-thiobarbituric acid and ultra-fast liquid chromatographic separation is described for the determination of malondialdehyde (MDA) in chicken liver, muscles and adipose tissue, and in lard and fish oil. Preparation of samples involves acid hydrolysis and derivatization. Separation is achieved using an Accucore C18-column (2.6 μm, Hydro-RP, 150×3.0 mm), an acetonitrile gradient in water, and detection at 530 nm. The results indicate that external calibration based on standard solutions of MDA may be used for measuring the MDA concentration in adipose tissue, lard and fish oil due to the absence of matrix effects. The MDA concentration in protein-rich biological samples should be calculated as the difference between the MDA concentration measured in MDA-spiked and unspiked samples of the same specimen and mass and the known concentration of the MDA spike. For liver or muscle samples, we suggest using external calibration based on standard solutions of MDA added to the same mass of liver or muscles. The proposed method is suitable for rapid and sensitive analysis of MDA in samples of animal origin or in plant oils. The method can also be suitable for routine evaluation of oxidative stress in animal tissues and oxidative stability of biological materials and animal products.
 
CITATIONS (10):
1.
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2.
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P. Konieczka, A. J. Rozbicka-Wieczorek, M. Czauderna, S. Smulikowska
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3.
The enrichment of chicken meat with omega-3 fatty acids by dietary fish oil or its mixture with rapeseed or flaxseed—Effect of feeding duration
P. Konieczka, M. Czauderna, S. Smulikowska
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4.
The interactive effect of dietary n-6: n-3 fatty acid ratio and vitamin E level on tissue lipid peroxidation, DNA damage in intestinal epithelial cells, and gut morphology in chickens of different ages
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5.
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7.
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9.
The effect of fish oil, lycopene and organic selenium as feed additives on rabbit meat quality
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10.
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X.L. Wu, X.Y. Zou, M. Zhang, H.Q. Hu, X.L. Wei, M.L. Jin, H.W. Cheng, S. Jiang
Poultry Science
 
ISSN:1230-1388