A rapid HPLC method for the determination of sulphonamides and trimethoprim in feed premixes
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University of Technology, Department of Agricultural Sciences, Biotechnology and Food Science, Limassol, P.O Box 50329, Cyprus
VitaTrace Nutrition Ltd, P.O Box 23886, Nicosia, Cyprus
Publication date: 2014-06-11
Corresponding author
O. Tzamaloukas   

University of Technology, Department of Agricultural Sciences, Biotechnology and Food Science, Limassol, P.O Box 50329, Cyprus
J. Anim. Feed Sci. 2014;23(2):185-189
A simple HPLC method for the simultaneous determination of sulphadimidine, sulphadiazine, sulphamethoxazole and trimethoprim antibiotics in medicated feeds has been developed, validated, and applied to commercial feed premixes. Ultrasound-assisted extraction was used for the recovery of the studied antibiotics without the need for a further clean-up step. The sulphonamides and trimethoprim were separated on a Symmetry C18 (5 μm, 4.6 × 250 mm) column using 10-min isocratic elution and UV detection at 254 nm. The results showed that the method is robust with adequate recovery (minimum 95.19%), satisfactory repeatability (1.39%–2.69%) and intermediate precision (1.36%–4.16%), as shown by the validation assessment herein. The advantages of the proposed method are the speed, the ease of the clean-up protocol, and the low cost of the needed instrumentation, rendering it highly applicable to the feed industry.
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