ORIGINAL PAPER
A rapid HPLC method for the determination of sulphonamides and trimethoprim in feed premixes
 
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1
University of Technology, Department of Agricultural Sciences, Biotechnology and Food Science, Limassol, P.O Box 50329, Cyprus
2
VitaTrace Nutrition Ltd, P.O Box 23886, Nicosia, Cyprus
CORRESPONDING AUTHOR
O. Tzamaloukas   

University of Technology, Department of Agricultural Sciences, Biotechnology and Food Science, Limassol, P.O Box 50329, Cyprus
Publication date: 2014-06-11
 
J. Anim. Feed Sci. 2014;23(2):185–189
 
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ABSTRACT
A simple HPLC method for the simultaneous determination of sulphadimidine, sulphadiazine, sulphamethoxazole and trimethoprim antibiotics in medicated feeds has been developed, validated, and applied to commercial feed premixes. Ultrasound-assisted extraction was used for the recovery of the studied antibiotics without the need for a further clean-up step. The sulphonamides and trimethoprim were separated on a Symmetry C18 (5 μm, 4.6 × 250 mm) column using 10-min isocratic elution and UV detection at 254 nm. The results showed that the method is robust with adequate recovery (minimum 95.19%), satisfactory repeatability (1.39%–2.69%) and intermediate precision (1.36%–4.16%), as shown by the validation assessment herein. The advantages of the proposed method are the speed, the ease of the clean-up protocol, and the low cost of the needed instrumentation, rendering it highly applicable to the feed industry.
 
CITATIONS (3):
1.
Tyrosinase based biosensor for the electrochemical determination of sulfamethoxazole
Lorena del Torno-de Román, M. Asunción Alonso-Lomillo, Olga Domínguez-Renedo, M. Julia Arcos-Martínez
Sensors and Actuators B: Chemical
 
2.
Green method by diffuse reflectance infrared spectroscopy and spectral region selection for the quantification of sulphamethoxazole and trimethoprim in pharmaceutical formulations
Fabiana E.B. da Silva, Érico M.M. Flores, Graciele Parisotto, Edson I. Müller, Marco F. Ferrão
Anais da Academia Brasileira de Ciências
 
3.
Spectrophotometric determination of drug compounds in pure forms and in the pharmaceutical preparations
Qabas N Rashid, Zeena T Khattab
IOP Conference Series: Materials Science and Engineering
 
ISSN:1230-1388