Glutathione redox state, glutathione peroxidase activity and selenium concentration in periparturient dairy cows, and their relation with negative energy balance
K. Mikulková 1  
,   J. Illek 1,   R. Kadek 1
More details
Hide details
University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Large Animal Clinical Laboratory, Brno 612 42, Czech Republic
K. Mikulková   

University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Large Animal Clinical Laboratory, Brno 612 42, Czech Republic
Publication date: 2020-03-31
J. Anim. Feed Sci. 2020;29(1):19–26
The aim of the study was to evaluate glutathione redox state, glutathione peroxidase (GPx) activity, and selenium (Se), non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB) concentrations in 15 Holstein periparturient dairy cows and to monitor the effect of negative energy balance (NEB) on the level of oxidative processes ongoing in dairy cows in postpartum period. The body condition score (BCS) was recorded and blood samples were collected 4 times during periparturient period. A significantly increased NEFA concentration was recorded on calving day (P < 0.05) and 7 days post partum (p.p.; P < 0.01) compared to 7 days ante partum (a.p.). The reduced glutathione (GSH) concentration was significantly decreased on calving day and 7 days p.p. (P < 0.05) as compared to 7 days a.p. The oxidized glutathione (GSSG) concentration was significantly higher 7 days p.p. as compared to calving day (P < 0.01) and 14 days p.p. (P < 0.05). Between the GSSG concentration and the GSH/GSSG ratio was found a significantly negative (r = −0.84; P < 0.001) correlation. The significant decrease in GPx activity was found 14 days p.p. as compared to 7 days a.p. (P < 0.05). The BCS value was significantly positively correlated (r = 0.44; P < 0.05) with GSSG concentration. The results of the study indicate significant changes of antioxidant/oxidant markers and also confirm that in the postpartum period oxidative stress occurs in dairy cows. It also seems that BCS correlates with these indicators and may influence the level of oxidative processes in cows during the periparturient period.
Avanzo J.L., de Mendonça C.X., Pugine S.M.P., Cesar M.C., 2001. Effect of vitamin E and selenium on resistance to oxidative stress in chicken superficial pectoralis muscle. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 129, 163–173, https://doi.org/10.1016/S1532-....
Bernabucci U., Ronchi B., Lacetera N., Nardone A., 2005. Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. J. Dairy Sci. 88, 2017–2026, https://doi.org/10.3168/jds.S0....
Cao L., Waldon D., Teffera Y., Roberts J., Wells M., Langley M., Zhao Z., 2013. Ratios of biliary glutathione disulfide (GSSG) to glutathione (GSH): a potential index to screen drug-induced hepatic oxidative stress in rats and mice. Anal. Bioanal. Chem. 405, 2635–2642, https://doi.org/10.1007/s00216....
Castillo C., Hernandez J., Bravo A., Lopez-Alonso M., Pereira V., Benedito J.L., 2005. Oxidative status during late pregnancy and early lactation in dairy cows. Vet. J. 169, 286–292, https://doi.org/10.1016/j.tvjl....
Cigliano L., Strazzullo M., Rossetti C., Grazioli G., Auriemma G., Sarubbi F., Iannuzzi C., Iannuzzi L., Spagnuolo M.S., 2014. Characterization of blood redox status of early and mid-late lactating dairy cows. Czech J. Anim. Sci. 59, 170–181, https://doi.org/10.17221/7341-....
Dobbelaar P., Bouwstra R.J., Goselink R.M.A., Jorritsma R., van den Borne J.J.G.C., Jansen E.H.J.M., 2010. Effects of vitamin E supplementation on and the association of body condition score with changes in peroxidative biomarkers and antioxidants around calving in dairy heifers. J. Dairy Sci. 93, 3103–3113, https://doi.org/10.3168/jds.20....
Elischer M.F., Sordillo L.M., Siegford J.M., Karcher E.L., 2015. Short communication: Characterizing metabolic and oxidant status of pastured dairy cows postpartum in an automatic milking system. J. Dairy Sci. 98, 7083–7089, https://doi.org/10.3168/jds.20....
Enkvetchakul B., Anthony N.B., Bottje W.G., 1995. Liver and blood glutathione in male broiler chickens, turkeys, and quall. Poult. Sci. 74, 885–889, https://doi.org/10.3382/ps.074....
Esposito G., Irons P.C., Webb E.C., Chapwanya A., 2014. Interactions between negative energy balance, metabolit diseases, uterine health and immune response in transitiv dairy cows. Anim. Reprod. Sci. 144, 60–71, https://doi.org/10.1016/j.anir....
Gong J., Xiao M., 2016. Selenium and antioxidant status in dairy cows at different stages of lactation. Biol. Trace Elem. Res. 171, 89–93, https://doi.org/10.1007/s12011....
Kaneko T., Iuchi Y., Kawachiya S., Fujii T., Saito H., Kurachi H., Fujii J., 2001. Alteration of glutathione reductase expression in the female reproductive organs during the estrous cycle. Biol. Reprod. 65, 1410–1416, https://doi.org/10.1095/biolre....
Konvičná J., Vargová M., Paulíková I., Kováč G., Kostecká Z., 2015. Oxidative stress and antioxidant status in dairy cows during prepartal and postpartal periods. Acta Vet. 84, 133–140, https://doi.org/10.2754/avb201....
Mayasari N., Chen J., Ferrari A., Bruckmaier R.M., Kemp B., Parmentier H.K., van Knegsel A.T.M., Trevisi E., 2016. Effects of dry period length and dietary energy source on inflammatory biomarkers and oxidative stress in dairy cows. J. Dairy Sci. 100, 4961–4975, https://doi.org/10.3168/jds.20....
Meister A., 1983. Selective modification of glutathione metabolism. Science 220, 472–477, https://doi.org/10.1126/scienc....
Meister A., Anderson M.E., 1983. Glutathione. Ann. Rev. Biochem. 52, 711–760, https://doi.org/10.1146/annure....
O’Boyle N., Corl C.M., Gandy J.C., Sordillo L.M., 2006. Relationship of body condition score and oxidant stress to tumor necrosis factor expression in dairy cattle. Vet. Immunol. Immunopathol. 113, 297–304, https://doi.org/10.1016/j.veti....
Omur A., Kirbas A., Aksu E., Kandemir F., Dorman E., Kaynar O., Ucar O., 2016. Effects of antioxidant vitamins (A, D, E) and trace elements (Cu, Mn, Se, Zn) on some metabolic and reproductive profiles in dairy cows during transition period. Pol. J. Vet. Sci. 19, 697–706, https://doi.org/10.1515/pjvs-2....
Paglia D.E., Valentine W.N., 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70, 158–169.
Pavlata L., Pechová A., Bečvár O., Illek J., 2001. Selenium status in cattle at slaughter: analyses of blood, skeletal muscle, and liver. Acta Vet. Brno 70, 277–284, https://doi.org/10.2754/avb200....
Pavlata L., Pechová A., Illek J., 2000. Direct and indirect assessment of selenium status in cattle – a comparison. Acta Vet. Brno 69, 281–287, https://doi.org/10.2754/avb200....
Pilarczyk B., Jankowiak D., Tomza-Marciniak A., Pilarczyk R., Sablik P., Drozd R., Tylkowska A., Skólmowska M., 2012. Selenium concentration and glutathione peroxidase (GSH-Px) activity in serum of cows at different stages of lactation. Biol. Trace Elem. Res. 147, 91–96, https://doi.org/10.1007/s12011....
Píšťková K., Kazatelová Z., Procházková H., Danielová L., Illek J., 2018. Antioxidant status and concentration levels of malondialdehyde (MDA) in dairy cows during periparturient period. Hungar. Vet. J. 140, Suppl. 1, 312–319.
Roche J.R., Friggens N.C., Kay J.K., Fisher M.W., Stafford K.J., Berry D.P., 2009. Invited review: Body condition score and its association with dairy cow productivity, health, and welfare. J. Dairy Sci. 92, 5769–5801, https://doi.org/10.3168/jds.20....
Sordillo L.M., 2005. Factors affecting mammary gland immunity and mastitis susceptibility. Livest. Prod. Sci. 98, 89–99, https://doi.org/10.1016/j.livp....
Sordillo L.M., Aitken S.L., 2009. Impact of oxidative stress on the health and immune function of dairy cattle. Vet. Immunol. Immunopathol. 128, 104–109, https://doi.org/10.1016/j.veti....
Sordillo L.M., O’Boyle N., Gandy J.C., Corl C.M., Hamilton E., 2007. Shifts in thioredoxin reductase activity and oxidant status in mononuclear cells obtained from transition dairy cattle. J. Dairy Sci. 90, 1186–1192, https://doi.org/10.3168/jds.S0....
Spears J.W., Weiss W.P., 2008. Role of antioxidant and trace elements in health and immunity of transition dairy cows. Vet. J. 176, 70–76, https://doi.org/10.1016/j.tvjl....
Tarin J.J., 1996. Potential effects of age-associated oxidative stress on mammalian oocytes/embryos. Mol. Hum. Reprod. 2, 717–724, https://doi.org/10.1093/molehr....
Tarin J.J., Ten J., Vendrell F.J., Cano A., 1998. Dithiothreitol prevents age-associated decrease in oocyte/conceptus viability in vitro. Mol. Hum. Reprod. 13, 381–386, https://doi.org/10.1093/humrep....
Tietze F., 1969. Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal. Biochem. 27, 502–522, https://doi.org/10.1016/0003-2....
The impact of the oxidative status on the reproduction of cows and the calves’ health – a review
Péter Hejel, János Sáfár, Barbara Bognár, László Kiss, Viktor Jurkovich, Endre Brydl, László Könyves
Acta Veterinaria Brno
Diet supplemented with lycopene and selenized yeast change contents of fatty acids in the liver and femoral muscles of rabbits
Marian Czauderna, Małgorzata Białek, Agnieszka Białek, Monika Karpińska
Livestock Science
Monitoring the redox status in dairy cows by using plasma dROMs, PAT, and OSI biomarkers
Péter Hejel, Viktor Jurkovich, Barbara Bognár, Péter Kovács, Endre Brydl, László Könyves
Acta Veterinaria Brno
Changes in the content and intensity of oxidation of lipid compounds in the kidneys of lambs fed diets with rapeseed and fish oils – effect of antioxidant supplementation
M. Białek, M. Czauderna, K. Zaworski, M. Karpińska, M. Marounek
Journal of Animal and Feed Sciences