0.906
IF5
0.875
IF
Q3
JCR
1.0
CiteScore
0.374
SJR
Q2
SJR
20
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY-NC 4.0
 
 

Glutathione redox state, glutathione peroxidase activity and selenium concentration in periparturient dairy cows, and their relation with negative energy balance

K. Mikulková 1  ,  
J. Illek 1,  
 
1
University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Large Animal Clinical Laboratory, Brno 612 42, Czech Republic
J. Anim. Feed Sci. 2020;29(1):19–26
Publication date: 2020-03-31
KEYWORDS
TOPICS
ABSTRACT
The aim of the study was to evaluate glutathione redox state, glutathione peroxidase (GPx) activity, and selenium (Se), non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB) concentrations in 15 Holstein periparturient dairy cows and to monitor the effect of negative energy balance (NEB) on the level of oxidative processes ongoing in dairy cows in postpartum period. The body condition score (BCS) was recorded and blood samples were collected 4 times during periparturient period. A significantly increased NEFA concentration was recorded on calving day (P < 0.05) and 7 days post partum (p.p.; P < 0.01) compared to 7 days ante partum (a.p.). The reduced glutathione (GSH) concentration was significantly decreased on calving day and 7 days p.p. (P < 0.05) as compared to 7 days a.p. The oxidized glutathione (GSSG) concentration was significantly higher 7 days p.p. as compared to calving day (P < 0.01) and 14 days p.p. (P < 0.05). Between the GSSG concentration and the GSH/GSSG ratio was found a significantly negative (r = −0.84; P < 0.001) correlation. The significant decrease in GPx activity was found 14 days p.p. as compared to 7 days a.p. (P < 0.05). The BCS value was significantly positively correlated (r = 0.44; P < 0.05) with GSSG concentration. The results of the study indicate significant changes of antioxidant/oxidant markers and also confirm that in the postpartum period oxidative stress occurs in dairy cows. It also seems that BCS correlates with these indicators and may influence the level of oxidative processes in cows during the periparturient period.
CORRESPONDING AUTHOR
K. Mikulková   
University of Veterinary and Pharmaceutical Sciences Brno, Faculty of Veterinary Medicine, Large Animal Clinical Laboratory, Brno 612 42, Czech Republic
 
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