sweet clover (Melilotus officinalis L.) is a legume species, widely used as a forage crop for herbivores. This study aimed to research the nutritional compositions of herbage and silage M. officinalis at vegetative, early flowering and full flowering stages. The crude protein (CP) and ash contents of M. officinalis herbages and silages in vegetative and early flowering stages were higher than those in the full flowering stage (P < 0.05). The linoleic and alpha-linolenic acid percentages in total fatty acids of M. officinalis herbages in vegetative and early flowering stages were greater than those in full flowering stage (P < 0.05). The neutral and acid detergent fibres without ash contents of M. officinalis herbages and silages at the full flowering stage were higher than those of vegetative and early flowering ones (P < 0.05). The malondialdehyde (MDA) concentrations (1.12–1.67 g/kg DM) of silages were higher than those (0.38–0.48 g/kg DM) of herbages. The in vitro gas production, metabolizable energy, net energy for lactation and organic matter digestibility values and the molarities of volatile acids in fermentation fluid of M. officinalis herbages were similar for all phenological stages (P > 0.05). The lactic acid contents of silages at vegetative and early flowering stages were higher than that of the full flowering one (P < 0.05). The linoleic acid contents of forages were negatively correlated with MDA forage content (P < 0.05). Consequently, the values of CP, alpha-linolenic acid and in vitro digestion for herbage, and the nutrient matter compositions of silage, in especially vegetative and early flowering stages, were at non-objectionable levels for quality forage. So, it is suggested that M. officinalis can be used as a good quality silage.
I would like to thank Ayşe Beren KARA for her assistance in collecting plant material and making silage.
The authors declare that there is no conflict of interest.
Allen O.N., Allen E.K., 1981. The Leguminosae: A Source Book of Characteristics, Uses and Nodulation. Madison, WI (USA).
Al Sherif E.A., 2009. Melilotus indicus (L.) All., a salt-tolerant wild leguminous herb with high potential for use as a forage crop in salt-affected soils. Flora 204, 737–746, https://doi.org/10.1016/j.flor....
Alonso V.A., Pereyra C.M., Keller L.A.M., Dalcero A.M., Rosa C.A.R., Chiacchiera S.M., Cavaglieri L.R., 2013. Fungi and mycotoxins in silage: an overview. J. Appl. Microbiol. 115, 637–643, https://doi.org/10.1111/jam.12....
Améndola-Massiotti R.D., González-Ortiz R., Miranda-Romero L.A., Burgueño-Ferreira J.A., Topete-Pelayo P., 2018. Ensiling alfalfa (Medicago sativa L.) and orchard grass (Dactylis glomerata L.) forage harvested at 08:00 or 14:00, without wilting or 1 or 2 h wilting and with or without use of bacterial inoculant. In: R.L. Edvan, E.M. Santos (Ediotors). Forage Groups. IntechOpen. London (UK), https://doi.org/10.5772/intech....
AOAC International, 1995. Official Methods of Analysis of AOAC International. 16th Edition. Arlington, VA (USA).
Ateş E., Seren O.A., 2020. Determination of forage yield and quality of blue melilot (Mellitus caeruleus (L.) Desr.) at different growth stages under Edirne ecological conditions. Ege Üniv. Ziraat Fak. Derg. 57, 111–117, https://doi.org/10.20289/zfder....
Barnett A.J.G., 1951. The colorimetric determination of lactic acid in silage. Biochem. J. 49, 527–529, https://doi.org/10.1042/bj0490....
Bernardes T.F., Gervásio J.R.S., De Morais G., Casagrande D.R., 2019. Technical note: a comparison of methods to determine pH in silages. 102, 9039–9042, https://doi.org/10.3168/jds.20....
Botsoglou N.A., Fletouris D.J., Papageorgious G.E., Vassilopoulos V.N., Mantis A.J., Trakatellis A.G., 1994. Rapid, sensitive, and specific thiobarbituric acid method for measuring lipid peroxidation in animal tissue, food, and feedstuff samples. J. Agric. Food Chem. 42, 1931–1937, https://doi.org/10.1021/jf0004....
Çaçan E., Aydın A., Başbağ M., 2015. Determination of quality features of some legume forage crops in Bingöl University Campus. Turk. J. Agric. Nat. Sci. 2, 105–111.
Canbolat Ö., Karaman Ş., 2009. Comparison of in vitro gas production, organic matter digestibility, relative feed value and metabolizable energy contents of some legume forages. J. Agric. Sci. 15, 188–195.
Cornara L., Xiao J., Burlando B., 2016. Therapeutic potential of temperate forage legumes: a Review. Crit. Rev. Food Sci. Nutr. 56, Suppl. 1, S149–S161, https://doi.org/10.1080/104083....
DaCosta M., Huang B., 2007. Changes in antioxidant enzyme activities and lipid peroxidation for bentgrass species in response to drought stress. J. Am. Soc. Hortic. Sci. 132, 319–326, https://doi.org/10.21273/JASHS....
Dierking R.M., Kallenbach R.L., Roberts C.A., 2010. Fatty acid profiles of orchardgrass, tall fescue, perennial ryegrass, and alfalfa. Crop Sci. 50, 391–402, https://doi.org/10.2135/cropsc....
Dumlu Z., Tan M., 2009. Determination of silage value of some legumes and mixtures in erzurum conditions (in Turkish) Atatürk Üniv. Ziraat Fak. Derg. 40, 15–21.
Ehlert K., 2020. Yellow sweet clover: information and management. https://extension.sdstate.edu/... (accessed at 01.05.2021).
Elgersma A., Ellen G., van der Horst H., Muuse B.G., Boer H., Tamminga S., 2003. Comparison of the fatty acid composition of fresh and ensiled perennial ryegrass (Lolium perenne L.), affected by cultivar and regrowth interval. Anim. Feed Sci. Technol. 108, 191–205, https://doi.org/10.1016/S0377-....
Ersahince A.C., Kara K., 2017. Nutrient composition and in vitro digestion parameters of Jerusalem artichoke (Helianthus tuberosus L.) herbage at different maturity stages in horse and ruminant. J. Anim. Feed Sci. 26, 213–225, https://doi.org/10.22358/jafs/....
Glasser F., Doreau M., Maxin G., Baumont R., 2013. Fat and fatty acid content and composition of forages: a meta-analysis. Anim. Feed Sci. Technol. 185, 19–34, https://doi.org/10.1016/j.anif....
Han L., Zhou H., 2013. Effects of ensiling processes and antioxidants on fatty acid concentrations and compositions in corn silages. J. Anim. Sci. Biotechnol. 4, 48, https://doi.org/10.1186/2049-1....
Jasicka-Misiak I., Makowicz E., Stanek N., 2017. Polish yellow sweet clover (Melilotus officinalis L.) honey, chromatographic fingerprints, and chemical markers. Molecules 22, 138, https://doi.org/10.3390/molecu....
Kara K., 2015. In vitro methane production and quality of corn silage treated with maleic acid. Ital. J. Anim. Sci. 14, 3994, https://doi.org/10.4081/ijas.2....
Kara K., 2020. Milk urea nitrogen and milk fatty acid compositions in dairy cows with subacute ruminal acidosis. Vet Med-Czech 65, 336–345, https://doi.org/10.17221/51/20....
Kara K., Aktuğ E., Özkaya S., 2016. Ruminal digestibility, microbial count, volatile fatty acids and gas kinetics of alternative forage sources for arid and semi-arid areas as in vitro. Ital. J. Anim. Sci. 15, 673–680, https://doi.org/10.1080/182805....
Kara K., Güçlü B.K., Baytok E., 2015. Comparison of nutrient composition and anti-methanogenic properties of different Rosaceae species. J. Anim. Feed Sci. 24, 308–314, https://doi.org/10.22358/jafs/....
Kara K., Ozkaya S., Baytok E., Guclu B.K., Aktug E., Erbas S., 2018. Effect of phenological stage on nutrient composition, in vitro fermentation and gas production kinetics of Plantago lanceolate herbage. Vet. Med. 63, 251–260, https://doi.org/10.17221/2/201....
Klebesadel L.J., 1992. Morphological, Physiological, and Winterhardiness Comparisons Among Latitudinal Ecotypes of Biennial Sweetclover (Melilotus Species) in Subarctic Alaska. Bulletin 91, http://hdl.handle.net/11122/12....
Lacey J., 1991. Natural occurrence of mycotoxins in growing and conserved forage crops. In: J.E. Smith, R. Henderson (Editors). Mycotoxins and Animal Foods. CRC Press. Boca Raton, FL (USA), pp. 363–397.
Luo K., Di H.Y., Zhang J.Y., Wang Y.R., Li Z.Q., 2014. Preliminary evaluation of agronomy and quality traits of nineteen Melilotus accessions. Pratacult. Sci. 8, 2125–2134, https://doi.org/10.11829/j.iss....
Menke K.H., Steingass H., 1988. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Develop. 28, 7–55.
Menke K.H., Raab L., Salewski A., Steingass H., Fritz D., Schneider W., 1979. The estimation of the digestibility and metabolizable energy content of ruminant feedstuffs from the gas production when they are incubated with rumen liquor in vitro. J. Agric. Sci. 93, 217–222, https://doi.org/10.1017/S00218....
Mir P.S., Bittman S., Hunt D., Entz T., Yip B., 2006. Lipid content and fatty acid composition of grasses sampled on different dates through the early part of the growing season. Can. J. Anim. Sci. 86, 279–290, https://doi.org/10.4141/A05-05....
National Academies of Sciences, Engineering, and Medicine, 2016. Nutrient Requirements of Beef Cattle. 8th Revised Edition. The National Academies Press. Washington, DC (USA), https://doi.org/10.17226/19014.
NRC (National Research Council), 2001. Nutrient Requirements of Dairy Cattle. 7th Revised Edition. The National Academies Press. Washington, DC (USA), https://doi.org/10.17226/9825.
Ogle D., St, John L., Tilley D., 2008. Plant Guide for yellow sweetclover (Melilotus officinalis (L.) Lam.) and white sweetclover (M. alba Medik). USDA-Natural Resources Conservation Service, Idaho Plant Materials Center. Aberdeen, ID (USA). 83210, https://www.nrcs.usda.gov/Inte....
Prasad T.K., 1996. Mechanisms of chilling-induced oxidative stress injury and tolerance in developing maize seedlings: changes in antioxidant system, oxidation of proteins and lipids, and protease activities. Plant J. 10, 1017–1026, https://doi.org/10.1046/j.1365....
Szumacher-Strabel M., Cieślak A., 2012. Dietary possibilities to mitigate rumen methane and ammonia production. In: G. Liu (Editor). Greenhouse Gases: Capturing, Utilisation and Reduction. IntechOpen. London (UK), https://doi.org/10.5772/32105.
Talbi S., Romero-Puertas M.C., Hernández A., Terrón L., Ferchichi A., Sandalio L.M., 2015. Drought tolerance in a Saharian plant Oudneya africana: role of antioxidant defences. Environ. Exp. Bot. 111, 114–126, https://doi.org/10.1016/j.enve....
Tekin M., Kara K., 2020. The forage quality and the in vitro ruminal digestibility, gas production, organic acids, and some estimated digestion parameters of tomato herbage silage with molasses and barley. Turk. J. Vet. Anim. Sci. 44, 201–213, https://doi.org/10.3906/vet-19....
Thompson J.E., Froese C.D., Madey E., Smith M.D., Hong Y., 1998. Lipid metabolism during plant senescence. Prog. Lipid Res. 37, 119–141, https://doi.org/10.1016/S0163-....
Turkington R.A., Cavers P.B., Rempel E., 1978. The biology of Canadian weeds. 29. Melilotus alba Desr. and M. officinalis (L.) Lam. Can. J. Plant Sci. 58, 523–537, https://doi.org/10.4141/cjps78....
Van Ranst G., Fievez V., Vandewalle M., De Riek J., Van Bockstaele E., 2009. Influence of herbage species, cultivar and cutting date on fatty acid composition of herbage and lipid metabolism during ensiling. Grass Forage Sci. 64, 196–207, https://doi.org/10.1111/j.1365....
Vanhatalo A., Kuoppala K., Toivonen V., Shingfield K.J., 2007. Effects of forage species and stage of maturity on bovine milk fatty acid composition. Eur. J. Lipid. Sci. Technol. 109, 856–867, https://doi.org/10.1002/ejlt.2....
Van-Soest P.J., Robertson J.B., Lewis B.A., 1991. Methods for dietary fiber, neutral detergent fiber and non- starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583–3597, https://doi.org/10.3168/jds.S0....
Wang J., Wu W., Wang X., Wang M., Wu F., 2015. An affective GC method for the determination of the fatty acid composition in silkworm pupae oil using a two-step methylation process. J. Serbian Chem. Soc. 80, 9–20, https://doi.org/10.2298/JSC140....
Yisehak K., 2008. Effect of seed proportions of Rhodes grass (Chloris gayana) and white sweet clover (Melilotus alba) at sowing on agronomic characteristics and nutritional quality. Livest. Res. Rural Develop. 20, 28.
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