Gut morphology of young pigs fed diets differing in standardized ileal digestible threonine and wheat gluten used as a source of non-essential amino acids
E. Święch 1  
,   M. Barszcz 1,   A. Tuśnio 1,   M. Taciak 1
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The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
E. Święch   

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
Publication date: 2016-08-25
J. Anim. Feed Sci. 2016;25(3):226–234
The aim of the study was to determine whether non-essential amino acids (NEAA) in form of wheat gluten (WG) have a threonine (Thr)- sparing effect on gut structure in young pigs. A two-factorial experiment was conducted on 12 groups of 6 pigs. Pigs of 12.5 kg initial body weight (BW) were fed for about 20 days diets differing in the Thr level (5.1, 5.7, 6.3 and 6.9 g standardized ileal digestible Thr · kg–1) and WG level (20.4, 40.4 and 60.4 g of WG protein · kg–1 of respective WG20, WG40 and WG60 diets). After slaughter at about 22.4 kg of BW, samples of duodenum, middle jejunum, ileum and proximal colon were taken for histological analysis. Morphology of all segments was not affected neither by Thr level nor by Thr and WG levels interaction, whereas effect of WG level differed among segments. In the duodenum, villi length and tunica mucosa thickness were decreased by WG60. In the duodenum and proximal colon, crypt depth was greater in WG20 than WG60 and/or WG40 groups, whereas in the ileum was greater in WG60 than WG20 group, and in the middle jejunum it was not affected. Villi length : crypt depth ratio was affected by WG only in the ileum, being greater in WG20 group. Myenteron was thicker in the ileum and proximal colon in WG60 than WG20 and WG40 group, respectively. The results do not evidence a sparing effect of NEAA provided by WG on Thr utilization for maintaining the proper gut structure in young pigs.
Bertolo R.F.P., Chen C.Z.L., Law G., Pencharz P.B., Ball R.O., 1998. Threonine requirement of neonatal piglets receiving total parenteral nutrition is considerably lower than that of piglets receiving an identical diet intragastrically. J. Nutr. 128, 1752–1759.
Boisen S., 1997. Ideal protein – and its suitability to characterize protein quality in pig feeds. A review. Acta Agr. Scand. Sect. A – Anim. Sci. 47, 31–38.
Burrin D.G., Stoll B., van Goudoever J.B., Reeds P.J., 2001. Nutrient requirements for intestinal growth and metabolism in the developing pigs. In: Proceedings of the 8th Symposium on Digestive Physiology of Pigs. Uppsala (Sweden), pp. 75–88.
Cera K.R., Maham D.C., Cross R.F., Reinhart G.A., Whitmoyer R.E., 1988. Effect of age, weaning and postweaning diet on small intestinal growth and jejunal morphology in young swine. J. Anim. Sci. 66, 574–584.
Dunsford B.R., Knabe D.A., Haensly W.E., 1989. Effect of dietary soybean meal on the microscopic anatomy of the small intestine in the early-weaned pig. J. Anim. Sci. 67, 1855–1863.
Gu X., Li D., 2004. Effect of dietary crude protein level on villous morphology, immune status and histochemistry parameters of digestive tract in weaning piglets. Anim. Feed Sci. Tech. 114, 113–226.
Hamard A., Sève B., Le Floc’h N., 2007. Intestinal development and growth performance of early-weaned piglets fed a low-threonine diet. Animal 1, 1134–1142.
Kitt S.J., Miller P.S., Fischer R.L., 2003. Effects of dietary glutamine on growth performance and small intestine weight and length of weanling pigs before and after an immune challenge. In: Proceedings of 9th Symposium on Digestive Physiology in Pigs. Banff (Canada), Vol. 2, pp. 337–339.
Law G.K., Bertolo R.F., Adjiri-Awere A., Pencharz P.B., Ball R.O., 2007. Adequate oral threonine is critical for mucin production and gut function in neonatal piglets. Am. J. Physiol. – Gastrointest. Liver Physiol. 292, G1293–G1301.
Montagne L., Pluske J.R., Hampson D.J., 2003. A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Anim. Feed Sci. Tech. 108, 95–117.
Nyachoti C.M., Omogbenigun F.O., Rademacher M., Blank G., 2006. Performance responses and indicators of gastrointestinal health in early-weaned pigs fed low-protein amino acid-supplemented diets. J. Anim. Sci. 84, 125–134.
Pluske J.R., Hampson D.J., Williams I.H., 1997. Factors influencing the structure and function of the small intestine in the weaned pig: a review. Livest. Prod. Sci. 51, 215–236.
Ren M., Liu X.T., Wang X., Zhang G.J., Qiao S.Y., Zeng X.F., 2014. Increased levels of standardized ileal digestible threonine attenuate intestinal damage and immune responses in Escherichia coli K88+challenged weaned piglets. Anim. Feed Sci. Tech. 195, 67-75.
StatSoft Inc., 2011. STATISTICA (data analysis software) Version 10, www.statsoft.com.
Stoll B., 2006. Intestinal uptake and metabolism of threonine: nutritional impact. Adv. Pork Prod. 17, 257–263.
Stoll B., Henry J., Reeds P.J., Yu H., Jahoor F., Burrin D.G., 1998. Catabolism dominates the first-pass intestinal metabolism of dietary essential amino acids in milk protein-fed piglets. J. Nutr. 128, 606–614.
Święch E., 2015. Utilization of threonine in young pigs fed diets differing in standardized ileal digestible threonine and wheat gluten used as a source of non-essential amino acids. J. Anim. Feed Sci. 24, 323–331.
Święch E., Boryczka M., Taciak M., Buraczewska L., 2011. The effect of graded levels of dietary threonine on nitrogen retention and structure of the small intestine in young pigs. J. Anim. Feed Sci. 20, 350–360.
Święch E., Buraczewska L., Tuśnio A., Taciak M., 2010. The effects of supplementing a low-protein threonine-deficient diet with different sources of non-essential amino acids on nitrogen retention and gut structure in young pigs. Arch. Anim. Nutr. 64, 22–35.
Wang W., Zeng X., Mao X., Wu G., Qiao S., 2010. Optimal dietary true ileal digestible threonine for supporting the mucosal barrier in small intestine of weaning pigs. J. Nutr. 140, 981–986.
Wang X., Qiao S.Y., Liu M., Ma Y.X., 2006. Effects of graded levels of true ileal digestible threonine on performance, serum parameters and immune function of 10-25 kg pigs. Anim. Feed Sci. Tech. 129, 264–278.
Wang X., Qiao S., Yin Y., Yue L., Wang Z., Wu G., 2007. A deficiency or excess of dietary threonine reduces protein synthesis in jejunum and skeletal muscle of young pigs. J. Nutr. 137, 1442–1446.
Wu G., Knabe D.A., Flynn N.E., 2005. Amino acid metabolism in the small intestine: biochemical bases and nutritional significance. In: D.G. Burrin, H.J. Mersmann (Editors). Biology of Metabolism in Growing Animals. Elsevier, Amsterdam (The Netherlands), pp. 107–126.
Goblet cells and mucus layer in the gut of young pigs: Response to dietary contents of threonine and non‐essential amino acids
Ewa Święch, Anna Tuśnio, Marcin Barszcz, Marcin Taciak, Ewelina Siwiak
Journal of Animal Physiology and Animal Nutrition