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Expression of 3β-hydroxysteroid dehydrogenase and P450 aromatase in porcine oviduct during the oestrous cycle

M. Martyniak 1,  
K. Zglejc 1,  
A. Franczak 1,  
G. Kotwica 1  
 
1
University of Warmia and Mazury in Olsztyn, Department of Animal Physiology, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland
J. Anim. Feed Sci. 2016;25(3):235–243
Publication date: 2016-08-25
DOI: https://doi.org/10.22358/jafs/65557/2016
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References (30) Citations (7)
 
KEYWORDS
oviduct, P450 aromatase, 3β-hydroxysteroid dehydrogenase, steroidogenesis, pig
ABSTRACT
Steroid hormones regulate the oviductal functions in cyclic pigs. The mRNA and protein steroidogenic enzymes expression – 3β-hydroxysteroid dehydrogenase (HSD3B), encoded by HSD3B and P450 aromatase (P450arom), encoded by CYP19, were studied in ampulla and isthmus of pig oviducts during luteal (days 2 - 3, 10 - 11 and 15 - 16) and follicular (days 18 - 20) phase. Expression of HSD3B in ampulla was greater on days 2 - 3, 12 - 13 and 18 - 20 vs 10 - 11 and 15 - 16. In isthmus it was greater on days 2 - 3 vs 10 - 11 and on days 18 - 20 vs 10 - 11 to 15 - 16. Proteins were immunolocalized in epithelial cells of ampulla and isthmus. On days 15 - 16, HSD3B concentration in ampulla was the greatest, in isthmus it was greater on days 15 - 16 vs 12 - 13. The P450arom protein expression was the greatest on days 2 - 3 in ampulla and isthmus and thereafter it gradually declined toward the end of the oestrous cycle. Progesterone concentration (pg · ml–1) in oviductal flushings did not change from days 2 - 3 (960.2 ± 367.2) to 15 - 16 (543.4 ± 235.3), but decreased on days 18 - 20 (154.5 ± 34.8). Androstenedione concentration (pg · ml–1) on days 2 - 3 (726.4 ± 108.4) and 18 - 20 (701.0 ± 59.0), and oestradiol-17β (pg · ml–1) levels on days 12-13 (104.1 ± 9.6) and 18-20 (110.4 ± 14.4) were greater than on other days. This study showed, that expression of mRNA and proteins of steroidogenic enzymes in oviduct of cyclic pigs depends on the stage of the oestrous cycle. This observation suggests ability of porcine oviduct to de novo synthesize steroid hormones.
CORRESPONDING AUTHOR
G. Kotwica   
University of Warmia and Mazury in Olsztyn, Department of Animal Physiology, Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland
 
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CITATIONS (7):
1. Interleukin-1 β system in the oviducts of pigs during the oestrous cycle and early pregnancy
M. Martyniak, A. Franczak, G. Kotwica
Theriogenology
2. Synthesis of steroid hormones in the porcine oviduct during early pregnancy
Marcin Martyniak, Anita Franczak, Genowefa Kotwica
Reproductive Biology
3. The expression profile of AQP1, AQP5 and AQP9 in granulosa and theca cells of porcine ovarian follicles during oestrous cycle and early pregnancy
P. Młotkowska, D. Tanski, M. Eliszewski, A. Skowronska, S. Nielsen, M. Skowronski
Journal of Animal and Feed Sciences
4. Transcriptomic analysis of the oviduct of pigs during the peri-conceptional period
Marcin Martyniak, Kamila Zglejc-Waszak, Anita Franczak, Genowefa Kotwica
Animal Reproduction Science
5. Expression of Chemerin and Its Receptors in the Porcine Hypothalamus and Plasma Chemerin Levels during the Oestrous Cycle and Early Pregnancy
Nina Smolinska, Marta Kiezun, Kamil Dobrzyn, Edyta Rytelewska, Katarzyna Kisielewska, Marlena Gudelska, Ewa Zaobidna, Krystyna Bogus-Nowakowska, Joanna Wyrebek, Kinga Bors, Grzegorz Kopij, Barbara Kaminska, Tadeusz Kaminski
International Journal of Molecular Sciences
6. Relative abundance of chemerin mRNA transcript and protein in pituitaries of pigs during the estrous cycle and early pregnancy and associations with LH and FSH secretion during the estrous cycle
Katarzyna Kisielewska, Edyta Rytelewska, Marlena Gudelska, Marta Kiezun, Kamil Dobrzyn, Krystyna Bogus-Nowakowska, Barbara Kamińska, Nina Smolinska, Tadeusz Kaminski
Animal Reproduction Science
7. Expression of chemerin receptors CMKLR1, GPR1 and CCRL2 in the porcine pituitary during the oestrous cycle and early pregnancy and the effect of chemerin on MAPK/Erk1/2, Akt and AMPK signalling pathways
Katarzyna Kisielewska, Edyta Rytelewska, Marlena Gudelska, Marta Kiezun, Kamil Dobrzyn, Krystyna Bogus-Nowakowska, Barbara Kamińska, Nina Smolinska, Tadeusz Kaminski
Theriogenology
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