Experimental intestinal stress induced by duodenal distention in sheep
More details
Hide details
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warsaw, Poland
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Publication date: 1999-03-19
J. Anim. Feed Sci. 1999;8(2):233-245
The effect of various degrees of 5 min duodenum distention (performed with a balloon filled with 20, 30, 40 or 80 ml of water, DD 20-DD 80) on spike bursts and motility of the forestomach and abomasum was investigated in sheep which, under general anaesthesia, had electrodes inserted into the muscular layers of the forestomachs, abomasum and duodenum, as well as into fistulas in the muscular layers of the rumen. Duodenal distention of DD 20 to DD 30 ml did not change the frequency of spike bursts or reticulo-ruminal motility, but significantly increased the amplitude of contractions (F=74.40 and F=110.02; P<0.001, respectively). In some animals a change in behaviour was observed (the animals shifted their weight from one foot to the other, urinated and/or defecated, looked around). Five min duodenal distention of DD 40 and DD 80 caused immediate and complete inhibition lasting for about 10 min of both the frequency of spike bursts and reticulo-ruminal contractions (F=43.46; P<0.001 and F=44.37; P<0.001, respectively) as well as of the amplitude of contractions (F= 142.25 and F=236.48; P<0.001, respectively). A l l animals showed the following signs: stretching their bodies, bleating, gnashing of teeth, lying down, urinating and defecating. Blood plasma Cortisol level increased significantly during gastrointestinal motility inhibition from 16.23±1.12 to 42.7±7.2 (F=58.88; P<0.001) during DD 40 and from 19.58±1.81 to 56.6 ±5.7 ng∙ml-1 30 min after the end of DD 80 ml (F=25.47; P<0.001). Duodenal distention inhibited forestomach motility proportionately to the degree of distention (F=31.87; P<0.001). This reaction is called the viscero-visceral inhibitory reflex. Moreover, it caused a change in animal behaviour and a significant increase in the plasma Cortisol concentration, testifying to the fact that this is a general reaction to pain as a stressor. The obtained results show that the model is sufficient to cause stress. It also allows the gradation of stress intensity. It can be concluded, therefore, that the above model can be used in testing anticolic drugs.
Quercetin Feeding in Newborn Dairy Calves Cannot Compensate Colostrum Deprivation: Study on Metabolic, Antioxidative and Inflammatory Traits
Jeannine Gruse, Ellen Kanitz, Joachim M. Weitzel, Armin Tuchscherer, Tadeusz Stefaniak, Paulina Jawor, Siegfried Wolffram, Harald M. Hammon, Antonio Gonzalez-Bulnes
Centrally administered PD 140.548 N-methyl-d-glucamine prevents the autonomic responses to duodenal pain in sheep
B.F. Kania, K. Kania, K. Romanowicz, D. Tomaszewska, V. Sutiak, D. Wronska-Fortuna
Research in Veterinary Science
Influence of centrally administered diltiazem on behavioural responses, clinical symptoms, reticulo-ruminal contractions and plasma catecholamine level after experimentally induced duodenal distension in sheep
B.F. Kania, V. Sutiak
Research in Veterinary Science
Validation of a method for assessment of an acute pain in lambs
Vince Molony, Joyce E Kent, Iain J McKendrick
Applied Animal Behaviour Science
Pain Relief - From Analgesics to Alternative Therapies
Kania Feliks, Danuta Wrońska
Large Animal Internal Medicine
Samuel Jones, Gilles Fecteau, Pamela Hullinger, Danelle Bickett-Weddle, Jean St., Sylvain Nichols, N. Maclachlan, Christie Mayo, Paul Walz, Sébastien Buczinski, Franklyn Garry, Craig McConnel, David Francoz, André Desrochers, Raymond Sweeney, Spring Halland, Robert Sager, Bradford Smith, Michelle Barton, Jack Easley, Anthony Blikslager, Gayle Hallowell, Liara Gonzalez, Kelsey Hart, Jennifer Davis, Nicola Pusterla, John Marshall, Tiffany Hall, Chris Sanchez, Robert Callan, Nimet Browne, Francisco Uzal
Journals System - logo
Scroll to top