1.054
IF5
1.150
IF
Q3
JCR
1.7
CiteScore
0.396
SJR
Q2
SJR
40
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Activity of phytate dephosphorylation in cereals, legumes and oilseeds determined by colourimetric and isotachophoretic method

M. Marounek 1, 2  ,  
I. Bubancová 2,  
D. Lukešová 3,  
 
1
Czech Academy of Sciences, Institute of Animal Physiology and Genetics, CZ-142 20 Prague, Czech Republic
2
Institute of Animal of Science, CZ-104 00 Prague, Czech Republic
3
Czech University of Life Sciences, Institute of Tropics and Subtropics, CZ-165 21 Prague, Czech Republic
J. Anim. Feed Sci. 2011;20(3):472–479
Publication date: 2011-08-11
KEYWORDS
ABSTRACT
Activity of phytate dephosphorylation was determined in 26 samples of cereals, legumes, oilseeds and complete diets for poultry using the colourimetric and isotachophoretic method. The former method was based on the colour formed upon the reaction of molybdate with phosphate released from phytate. The latter method was based on measurement of decrease of phytate concentration in the reaction mixture. Rye had by far the highest phytate dephosphorylation activity, followed by wheat and wheat-containing diets. However, for most of the other samples, this activity was low. Phytate dephosphorylation activity as measured by capillary isotachophoresis was numerically lower than activity measured using the molybdate method; however, activities assayed by both methods correlated with statistical significance (r=0.86; P<0.001). The isotachophoretic method may be suitable for phytate dephosphorylation activity assay in feeds with a high content of inorganic phosphate and in samples of digesta and faeces.
CORRESPONDING AUTHOR
M. Marounek   
Czech Academy of Sciences, Institute of Animal Physiology and Genetics, CZ-142 20 Prague, Czech Republic; Institute of Animal of Science, CZ-104 00 Prague, Czech Republic
ISSN:1230-1388