Removal of fat from crushed black soldier fly larvae by carbon dioxide supercritical extraction
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Gene Analysis Center, Gyeongnam National University of Science and Technology, 33 Dongjin-ro, Jinju-city 52725, South Korea
Laboratory of Aquatic Animal Diseases, College of Veterinary Medicine, Gyeongsang National University, 501 Jinju-daero, Jinju-city 52828, South Korea
Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, 33 Dongjin-ro, Jinju-city 52725, South Korea
Gyeongsangnam-do Agricultural Research and Extension Services, 570 Daesin-ro, Jinju-city, 52733, South Korea
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, 33 Dongjin-ro, Jinju-city 52725, South Korea
Inland Aquaculture Research Center, National Institute of Fisheries Science, 645-806 Gyeongnam, South Korea
Korea Aquatic Life Institute Co.Ltd, Geumjeong-ro 90, Geumjeong-ku, Busan-city 46290, South Korea
J. H. Yoo   

Korea Aquatic Life Institute Co.Ltd, Geumjeong-ro 90, Geumjeong-ku, Busan-city 46290, South Korea
Publication date: 2019-03-22
J. Anim. Feed Sci. 2019;28(1):83–88
The larvae of the black soldier fly, Hermetia illucens, contain high amounts of protein and fat, and so are a good feed source. However, this high fat content hinders the production of such feed. The excess fat must thus be removed to enable the larvae to be used as a feedstock e.g., for aquaculture and livestock. Firstly, the use of hot water treatment as a strategy for extracting fat from dried larvae was tested. It was found that this process reduced the fat content of the raw material (from 30 to about 16%). However, the resulting product was still not suitable for feed processing. Next, the use of a carbon dioxide supercritical extraction system on crushed larvae was studied. This system time-dependently reduced the fat content to less than 6.6% and less than 4.6% after treatments for 2 and 6 h, respectively, with a pressure of 350 bar used on larval particles of 10–18 mesh in size. Therefore, subjecting 10–18 mesh crushed larval powder to carbon dioxide supercritical extraction at 350 bar for 6 h yielded a larval powder with a fat content of 5% or less. Based on this finding, this method is proposed to be a suitable alternative for further processing of larvae feedstock.
AOAC International, 2016. Official Methods of Analysis of AOAC International. 20th Edition. Rockville, MD (USA).
Bravo-Tello K., Ehrenfeld N., Solís C.J., Ulloa P.E., Hedrera M., Pizarro-Guajardo M., Paredes-Sabja D., Feijóo C.G., 2017. Effect of microalgae on intestinal inflammation triggered by soybean meal and bacterial infection in zebrafish. PLoS One 12, e0187696,
Čičková H., Newton G.L., Lacy R.C., Kozánek M., 2015. The use of fly larvae for organic waste treatment. Waste Manag. 35, 68–80,
Gu M., Bai N., Xu B., Xu X., Jia Q., Zhang Z., 2017. Protective effect of glutamine and arginine against soybean meal-induced enteritis in the juvenile turbot (Scophthalmus maximus). Fish Shellfish Immunol. 70, 95–105,
Józefiak A., Kierończyk B., Rawski M., Mazurkiewicz J., Benzertiha A., Gobbi P., Nogales-Merida S., Świątkiewicz S., Józefiak D., 2018. Full-fat insect meals as feed additive – the effect on broiler chicken growth performance and gastrointestinal tract microbiota. J. Anim. Feed Sci. 27, 131–139,
Lee J.I., Kye B.M., 1970. Effect of oil quality by extracting hour on rape. Res. Rept. RDA (C.P.) 13, 89–94.
NAAS, 2010. Fertilizer Application Recommendations for Crop Plants. National Academy of Agricultural Science (NAAS), RDA, Suwon, Korea.
Nowak V., Persijn D., Rittenschober D., Charrondiere U.R., 2016. Review of food composition data for edible insects. Food Chem. 193, 39–46,
Pan W.J., Liao A.M., Zhang J.G., Dong Z., Wei Z.J., 2012. Supercritical carbon dioxide extraction of the oak silkworm (Antheraea pernyi) pupal oil: process optimization and composition determination. Int. J. Mol. Sci. 13, 2354–2367,
Perera E., Yúfera M., 2017. Effects of soybean meal on digestive enzymes activity, expression of inflammation-related genes, and chromatin modifications in marine fish (Sparus aurata L.) larvae. Fish Physiol. Biochem. 43, 563–578,
Purschke B., Stegmann T., Schreiner M., Jäger H., 2017. Pilot-scale supercritical CO2 extraction of edible insect oil from Tenebrio molitor L. larvae – Influence of extraction conditions on kinetics, defatting performance and compositional properties. Eur. J. Lipid Sci. Technol. 119, 1–12,
Rothman J.M., Raubenheimer D., Bryer M.A., Takahashi M., Gilbert C.C., 2014. Nutritional contributions of insects to primate diets: Implications for primate evolution. J. Hum. Evol. 71, 59–69,
Rumpold B.A., Schlüter O.K., 2013a. Potential and challenges of insects as an innovative source for food and feed production. Innov. Food Sci. Emerg. Technol. 17, 1–11,
Rumpold B.A., Schlüter O.K., 2013b. Nutritional composition and safety aspects of edible insects. Mol. Nutr. Food Res. 57, 802–823,
Spranghers T., Ottoboni M., Klootwijk C., Ovyn A., Deboosere S., De Meulenaer B., Michiels J., Eeckhout M., De Clercq P., De Smet S., 2017. Nutritional composition of black soldier fly (Hermetia illucens) prepupae reared on different organic waste substrates. J. Sci. Food Agric. 97, 2594–2600,
van Huis A., Van Itterbeeck J., Klunder H., Mertens E., Halloran A., Muir G., Vantomme P., 2013. Edible Insects. Future prospects for food and feed security. FAO Forestry Paper 171, Rome (Italy),
Verkerk M.C., Tramper J., van Trijp J.C.M., Martens D.E., 2007. Insect cells for human food. Biotechnol. Adv. 25, 198–202,
Reference Module in Food Science
Anandharamakrishnan C
Reference Module in Food Science
Hossain Sohrab, Siti Mohamed, Khalid Binti, Venugopal Balakrishnan, Islam Zaidul, Mohd Kadir
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