CC-BY 4.0

Removal of fat from crushed black soldier fly larvae by carbon dioxide supercritical extraction

S. W. Kim 1,  
T. S. Jung 2,  
Y. J. Ha 3,  
S. W. Gal 3,  
C. W. Noh 4,  
I. S. Kim 5,  
J. H. Lee 6,  
J. H. Yoo 7  
Gene Analysis Center, Gyeongnam National University of Science and Technology, 33 Dongjin-ro, Jinju-city 52725, South Korea
Laboratory of Aquatic Animal Diseases, College of Veterinary Medicine, Gyeongsang National University, 501 Jinju-daero, Jinju-city 52828, South Korea
Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology, 33 Dongjin-ro, Jinju-city 52725, South Korea
Gyeongsangnam-do Agricultural Research and Extension Services, 570 Daesin-ro, Jinju-city, 52733, South Korea
Swine Science and Technology Center, Gyeongnam National University of Science & Technology, 33 Dongjin-ro, Jinju-city 52725, South Korea
Inland Aquaculture Research Center, National Institute of Fisheries Science, 645-806 Gyeongnam, South Korea
Korea Aquatic Life Institute Co.Ltd, Geumjeong-ro 90, Geumjeong-ku, Busan-city 46290, South Korea
J. Anim. Feed Sci. 2019;28(1):83–88
Publication date: 2019-03-22
The larvae of the black soldier fly, Hermetia illucens, contain high amounts of protein and fat, and so are a good feed source. However, this high fat content hinders the production of such feed. The excess fat must thus be removed to enable the larvae to be used as a feedstock e.g., for aquaculture and livestock. Firstly, the use of hot water treatment as a strategy for extracting fat from dried larvae was tested. It was found that this process reduced the fat content of the raw material (from 30 to about 16%). However, the resulting product was still not suitable for feed processing. Next, the use of a carbon dioxide supercritical extraction system on crushed larvae was studied. This system time-dependently reduced the fat content to less than 6.6% and less than 4.6% after treatments for 2 and 6 h, respectively, with a pressure of 350 bar used on larval particles of 10–18 mesh in size. Therefore, subjecting 10–18 mesh crushed larval powder to carbon dioxide supercritical extraction at 350 bar for 6 h yielded a larval powder with a fat content of 5% or less. Based on this finding, this method is proposed to be a suitable alternative for further processing of larvae feedstock.
J. H. Yoo   
Korea Aquatic Life Institute Co.Ltd, Geumjeong-ro 90, Geumjeong-ku, Busan-city 46290, South Korea
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