1.054
IF5
1.150
IF
Q3
JCR
1.7
CiteScore
0.396
SJR
Q2
SJR
40
MNiSW
148.75
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Tissue-specific effect of recombinant porcine growth hormone on expression of growth-related genes in the pig

R. Q. Zhao 1,  
J. Zhou 1,  
Q. F. Xu 1,  
Z. H. Zhao 1,  
X. H. Wei 1,  
D. Xia 1,  
W. H. Chen,  
J. Chen 1  
 
1
Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing 210095, P. R. China
J. Anim. Feed Sci. 2003;12(3):605–615
Publication date: 2003-07-15
KEYWORDS
ABSTRACT
Sixteen castrated male Large White × Landrace pigs were injected intramuscularly with recombinant porcine growth hormone (rpGH, 4 mg · d-1, n=8) or saline (n=8) for 28 days. Animals were slaughtered 4 h after the final injection for tissue and blood sampling. RIA and semi-quantitative RT-PCR were employed for determining hormone and mRNA contents, respectively. rpGH administration enhanced average daily weight gain by 26.07% (P<0.05), elevated serum IGF-I concentration by 70.94% (P<0.01) but decreased serum level of leptin by 34.52% (P<0.01). The relative abundance of GH-R mRNA was increased by 24.45% (P<0.05) in liver, 50.66% (P<0.05) in adipose tissue, 21.29% (P<0.05) in semitendinosus (ST), but not in longissimus dorsi (LD) muscle. Coordinately, IGF-1 mRNA expression was enhanced by 45.30% (P<0.01) in liver, 27.82% (P<0.05) in adipose tissue, 41.04% (P<0.05) in ST, but not in LD muscle. The IGF-1 content in liver and ST were increased 2.97 and 5.53-fold, respectively. A significant (2.93-fold) increase of IGF-1 content was found in LD muscle too, uncoupling with the change in mRNA level. Moreover, rpGH administration augmented IGF-IR mRNA expression by 48.08% (P<0.01) whereas abated leptin mRNA expression by 28.19% (P<0.05) in adipose tissue. These results suggest that the effect of rpGH on GH-R and IGF-I gene expression is tissue-specific and rpGH administration may down-regulate leptin mRNA expression in adipose tissue either directly or via the mediation of local IGF-I/IGF-IR system.
CORRESPONDING AUTHOR
J. Chen   
Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing 210095, P. R. China
 
CITATIONS (3):
1. Effect of chicken egg yolk antibody against adipose tissue plasma membranes on carcass composition and lipogenic hormones and enzymes in pigs
J.P. Jiang, J. Zhou, J. Chen, X.H. Wei, T.S. Lu, H. Chi, R.Q. Zhao
Livestock Science
2. Muscle type-specific responses of myoD and calpain 3 expression to recombinant porcine growth hormone in the pig
X. Yang, J. Chen, Q. Xu, R. Zhao
animal
3. Expression of GHR and PGC-lα in association with changes of MyHC isof orm types in longissimus muscle of Erhualian and Large White pigs (Sus scrofa) during postnatal growth
Ru-Qian Zhao, Xiao-Jing Yang, Qin-Fu Xu, Xi-Hui Wei, Dong Xia, Jie Chen
Animal Science
ISSN:1230-1388