0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

The importance of washing the omasum for successful defaunation of sheep

J. Harmeyer 2,  
 
1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
2
Institute of Physiology, School of Veterinary Medicine, Bischofsholer Damm 15/102, 30173 Hannover, Germany
J. Anim. Feed Sci. 1999;8(4):611–619
Publish date: 1999-10-07
KEYWORDS
ABSTRACT
Four different methods for defaunating were tested. Small Entodinia but some times also other species of ciliates were found on the next day after defaunation when Aerosol OT was given in doses of 5 and 10 g/animal or 1-1.5 g/kg of rumen content. Emptying and washing the reticulorumen with a 0.9% NaCl solution and subsequent freezing of the contents to -20°C for 96 h resulted in absence of protozoa for a period of 10-14 days and this was followed by the re-establishment of a population of Eudiplodinium maggii, Entodinium simplex and Dasytricha ruminantium. Emptying and washing of the reticulo-rumen with warm (40°C) water accompanied by heating of the contents to 70°C and addition of 10 g of Aerosol OT or 5 g sodium lauryl sulphate (SDS) eliminated the ciliates from the rumen for a period of 7-40 days but re-establishment of small protozoa like Entodinium simplex and Entodinium caudatum was observed later. Emptying the reticulo-rumen and washing the omasum, reticulum and rumen with warm water followed by heating of the contents to 70°C and the addition of Aerosol OT at a proportion of 1 g/kg digesta resulted in elimination of all the species of ciliates from the reticulo-rumen of all defaunated sheep for a period longer than 70 days.
 
CITATIONS (13):
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A. Marcin, K.-H. Südekum
Journal of Animal Physiology and Animal Nutrition
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R. Miltko, G. Bełżecki, E. Kwiatkowska, T. Michałowski
Folia Microbiologica
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Folia Microbiologica
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Grzegorz Bełżecki, Neil R. McEwan, Barbara Kowalik, Tadeusz Michałowski, Renata Miltko
European Journal of Protistology
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G. Bełżecki, R. Miltko, T. Michalowski
Folia Microbiologica
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Grzegorz Belzecki, Renata Miltko, Tadeusz Michalowski
Folia Microbiologica
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Johanna O. Zeitz, Sergej L. Amelchanka, Tadeusz Michałowski, Krzysztof Wereszka, Leo Meile, Sonja Hartnack, Michael Kreuzer, Carla R. Soliva
Archives of Animal Nutrition
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G. Bełżecki, R. Miltko, E. Kwiatkowska, T. Michałowski
Folia Microbiologica
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Grzegorz Bełżecki, Renata Miltko, Tadeusz Michałowski, Neil R. McEwan, Albert Descoteaux
FEMS Microbiology Letters
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Renata Miltko, Marta Pietrzak, Grzegorz Bełżecki, Krzysztof Wereszka, Tadeusz Michałowski, Johannes H.P. Hackstein
European Journal of Protistology
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R. Miltko, G. Belzecki, T. Michalowski
Folia Microbiologica
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Marian Czauderna, Krzysztof Wereszka, Tadeusz Michałowski
European Journal of Protistology
ISSN:1230-1388