Four different methods for defaunating were tested. Small Entodinia but some times also other
species of ciliates were found on the next day after defaunation when Aerosol OT was given in
doses of 5 and 10 g/animal or 1-1.5 g/kg of rumen content. Emptying and washing the reticulorumen with a 0.9% NaCl solution and subsequent freezing of the contents to -20°C for 96 h resulted
in absence of protozoa for a period of 10-14 days and this was followed by the re-establishment of a
population of Eudiplodinium maggii, Entodinium simplex and Dasytricha ruminantium. Emptying
and washing of the reticulo-rumen with warm (40°C) water accompanied by heating of the contents
to 70°C and addition of 10 g of Aerosol OT or 5 g sodium lauryl sulphate (SDS) eliminated the
ciliates from the rumen for a period of 7-40 days but re-establishment of small protozoa like Entodinium simplex and Entodinium caudatum was observed later.
Emptying the reticulo-rumen and washing the omasum, reticulum and rumen with warm water
followed by heating of the contents to 70°C and the addition of Aerosol OT at a proportion of 1 g/kg
digesta resulted in elimination of all the species of ciliates from the reticulo-rumen of all defaunated
sheep for a period longer than 70 days.
CITATIONS(13):
1.
Nutritive defaunation of the rumen in steers with subsequent refaunation using a cryopreserved monoculture ofEntodinium caudatum A. Marcin, K.-H. Südekum Journal of Animal Physiology and Animal Nutrition
The effect of rumen ciliates on chitinolytic activity, chitin content and the number of fungal zoospores in the rumen fluid of sheep Renata Miltko, Grzegorz Bełżecki, Andrzej Herman, Barbara Kowalik, Jacek Skomiał Archives of Animal Nutrition
The ability of rumen ciliates, Eudiplodinium maggii, Diploplastron affine, and Entodinium caudatum, to use the murein saccharides G. Belzecki, R. Miltko, E. Kwiatkowska, T. Michalowski Folia Microbiologica
Effect of Entodinium caudatum on starch intake and glycogen formation by Eudiplodinium maggii in the rumen and reticulum Grzegorz Bełżecki, Neil R. McEwan, Barbara Kowalik, Tadeusz Michałowski, Renata Miltko European Journal of Protistology
Why does the establishment of the starch preferringEntodinium caudatum in the rumen decrease the numbers of the fibrolytic ciliateEudiplodinium maggii? G. Bełżecki, R. Miltko, T. Michalowski Folia Microbiologica
Effect of the rumen ciliatesEntodinium caudatum, Epidinium ecaudatumandEudiplodinium maggii, and combinations thereof, on ruminal fermentation and total tract digestion in sheep Johanna O. Zeitz, Sergej L. Amelchanka, Tadeusz Michałowski, Krzysztof Wereszka, Leo Meile, Sonja Hartnack, Michael Kreuzer, Carla R. Soliva Archives of Animal Nutrition
Methods for the cultivation of ciliated protozoa from the large intestine of horses Grzegorz Bełżecki, Renata Miltko, Tadeusz Michałowski, Neil R. McEwan, Albert Descoteaux FEMS Microbiology Letters
Isolation and in vitro cultivation of the fibrolytic rumen ciliate Eremoplastron (Eudiplodinium) dilobum Renata Miltko, Marta Pietrzak, Grzegorz Bełżecki, Krzysztof Wereszka, Tadeusz Michałowski, Johannes H.P. Hackstein European Journal of Protistology
The utilization and digestion of cellulose by the rumen ciliate Diploplastron affine Marian Czauderna, Krzysztof Wereszka, Tadeusz Michałowski European Journal of Protistology
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