1.054
IF5
1.150
IF
Q3
JCR
1.7
CiteScore
0.396
SJR
Q2
SJR
40
MNiSW
148.75
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Selenite and selenate affected the fatty acid profile in in vitro incubated ovine ruminal fluid containing linoleic acid

M. Czauderna 1  ,  
J. Kowalczyk 1,  
 
1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
2
Rowett Institute of Nutrition and Health, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK
J. Anim. Feed Sci. 2012;21(3):477–492
Publication date: 2012-09-29
KEYWORDS
ABSTRACT
The influence of adding selenite (SeIV) or selenate (SeVI) to ovine ruminal fluid containing linoleic acid (LA) on the profile of fatty acids, especially conjugated linoleic acid (CLA) isomers and their metabolites was investigated. Dietary LA is incorporated by rumen bacteria, isomerized to other geometric and positional isomers, metabolized into CLA isomers, biohydrogenated to trans-vaccenic acid (TVA) and finally to C18:0. Considering the above, ovine ruminal fluid was incubated in vitro at 39°C under CO2 either alone (the control ruminal fluid) or with a combination of LA (1.67 mg/ml), a low (0.167 μg/ml) or high (1.67 μg/ml) level of selenium as SeIV or SeVI. Tubes with examined ruminal fluid were removed after 0, 6, 12, 18, and 24 hrs of incubation and then submitted for determination of fatty acids (FA). FA, as methyl esters, were quantitated using capillary gas chromatography and flame-ionization detection. Both concentrations of SeIV added to the ruminal fluid with LA usually decreased the concentrations of individual CLA isomers, especially cis9trans11CLA (c9t11CLA) and the sum of all CLA isomers in the ruminal fluid in comparison with the fluid containing only LA. Our studies documented that SeIV reduced the capacity of bacterial isomerase, which turns the cis9-bond into a trans10-bond. The addition of SeIV to the ruminal fluid with LA decreased the concentration of TVA compared with the fluid with only LA; a decrease in the loss of TVA was observed with increasing concentrations of SeIV. The presence of SeIV in the ovine fluid with LA stimulated the biohydrogenation of TVA to C18:0. The addition of LA to the incubated fluid, irrespectively of the presence of SeIV, increased the concentration of C20:5n-3. SeVI in the ruminal fluid with LA usually more efficiently increased the concentration of c9t11CLA, t10c12CLA, c9c11CLA and t9t11CLA, from 6 until 24 hrs of incubation compared with the fluid containing LA, regardless of the presence of SeIV. The concentration of TVA in the fluid containing SeVI and LA is higher than in the fluid with SeIV and LA. SeVI in the fluid increased the concentration of C18:0. As a consequence, SeVI added to the fluid increased the yield of final biohydrogenation to C18:0 compared with the fluid with LA, irrespective of the presence of SeVI. Further studies are required to clarify the effects of other Se-compounds and fatty acids on concentrations of fatty acids, especially CLA isomers and their precursors, in the ruminal fluid.
CORRESPONDING AUTHOR
M. Czauderna   
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
 
CITATIONS (5):
1. Seleno-methionine decreases biohydrogenation of C18 unsaturated fatty acids in ovine ruminal fluid incubated in vitro with α-linolenic acid
Marian Czauderna, Agnieszka J. Rozbicka-Wieczorek, Edyta Więsyk, Katarzyna A. Krajewska-Bienias
European Journal of Lipid Science and Technology
2. Selenium species in diet containing carnosic acid, fish and rapeseed oils affect fatty acid profiles in lamb muscles
A. Rozbicka-Wieczorek, M. Czauderna, E. Więsyk, A. Radzik-Rant
Journal of Animal and Feed Sciences
3. Supplementation effects of seleno-compounds, carnosic acid, and fish oil onconcentrations of fatty acids, tocopherols, cholesterol, and amino acids in the livers of lambs
Agnieszka Justyna ROZBICKA-WIECZOREK, Edyta WIESYK, Katarzyna Agnieszka KRAJEWSKA-BIENIAS, Krzysztof WERESZKA, Marian CZAUDERNA
TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES
4. Process performance and microbial communities in response to selenite addition during anaerobic digestion of pig manure
Y. Liang, J. Bao, J. Ding, J. Tang, W. Li, L. Zhang, Y. Zhang
International Journal of Environmental Science and Technology
5. Selenate and selenite affect ruminal metabolism of C18 unsaturated fatty acids and fatty acid composition of lamb tissues
Małgorzata Białek, Professor Czauderna, Wiesław Przybylski, Danuta Jaworska
Livestock Science
ISSN:1230-1388