SSCP polymorphism within a promoter of the bovine alpha S1 casein gene
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Department of Animal Genetics, University of Warmia and Masuria in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland
Publication date: 2000-01-13
J. Anim. Feed Sci. 2000;9(1):73–79
The detection of a new SSCP polymorphism within the promoter of the bovine alpha S1 casein gene is described. The fragment of alpha S1 casein gene promoter (218 bp) was PCR-amplified and then subjected to electrophoresis allowing the detection of SSCP polymorphism. Among 118 randomly chosen cows (Black-and-White) and 124 bulls (Black-and-White), we found two SSCP patterns, labelled R2 and R3, with respective frequencies of 0.05 and 0.95. The detected SSCP polymorphism may be useful in searching for associations between different SSCP patterns and gene expression, especially milk protein percentage and protein yield.