0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Cellular localization and putative role of aquaporin-2 Ser-261 in the bovine kidney

K. Michałek 1  ,  
M. Grabowska 2,  
 
1
West Pomeranian University of Technology in Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Physiology, Cytobiology and Proteomics, Klemensa Janickiego 29, 71-270 Szczecin, Polan
2
Pomeranian Medical University, Department of Histology and Developmental Biology, Żołnierska 48, 71-210 Szczecin, Poland
J. Anim. Feed Sci. 2019;28(1):15–21
Publish date: 2019-02-28
KEYWORDS
TOPICS
ABSTRACT
Aquaporin-2 (AQP2) is a small, transmembrane protein, active in the principal cells of the renal collecting duct system. Phosphorylation and dephosphorylation of this protein regulate its redistribution in the cell, thereby influencing renal water reabsorption. The aims of the study were to identify and analyse the location of AQP2 phosphorylated at position 261, and to determine its potential role in renal water balance regulation, because data on AQP2 in cattle are scarce, and the mechanisms regulating its expression and cellular localization remain unclear. The analyses were carried out on kidneys collected from eight Polish Holstein-Friesian Black-and-White male calves, aged 5–7 months. It was found, using immunohistochemistry and commercially available phosphospecific antibodies, that in cattle AQP2 Ser-261 is located exclusively in the apical plasma membrane of the principal cells of the collecting duct. This atypical location of AQP2 Ser-261 allows to conclude that it may play a significant role in the process of renal water retention. The results of our previous studies on the regulation of renal total AQP2 excretion with urine in calves, as well as the bioinformatic analysis of available data presented in this study, seem to support this assumption. In addition, bioinformatic tools predicted mitogen activated protein kinases (MAPK) as a possible vasopressin-independent factor involved in AQP2 Ser-261 phosphorylation and accumulation in the apical plasma membrane.
CORRESPONDING AUTHOR
K. Michałek   
West Pomeranian University of Technology in Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Physiology, Cytobiology and Proteomics, Klemensa Janickiego 29, 71-270 Szczecin, Poland
 
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