ORIGINAL PAPER
An analysis of MYH7 single nucleotide polymorphism (g.7:75667956G>A) in relation to growth and carcass traits in pigs
1
West Pomeranian University of Technology Szczecin, Faculty of Biotechnology and Animal Husbandry,
Department of Genetics and Animal Breeding, al. Piastów 45, 70-311 Szczecin, Poland
2
National Research Institute of Animal Production, Department of Animal Genetics and Breeding,
Krakowska 1, 32-083 Balice near Krakow, Poland
Publication date: 2018-11-28
Corresponding author
D. Polasik
West Pomeranian University of Technology Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Genetics and Animal Breeding, al. Piastów 45, 70-311 Szczecin, Poland
West Pomeranian University of Technology Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Genetics and Animal Breeding, al. Piastów 45, 70-311 Szczecin, Poland
J. Anim. Feed Sci. 2018;27(4):335-340
KEYWORDS
TOPICS
ABSTRACT
The aim of this study was to evaluate MYH7 single nucleotide
polymorphism (SNP) (NC_010449.4: g.7:75667956G>A) in relation to growth
and carcass traits in pigs reared in Poland. Previous study has shown that
g.7:75667956G>A substitution in pigs influences miR-208b and MYH7 expression,
and is associated with proportion of muscle fibre types. The presented
study was conducted on 582 pigs belonging to six breeds: Polish Landrace,
Polish Large White, Puławska, Pietrain, Duroc and Hampshire. Statistical analysis
(GLM procedure) was performed for first three breeds separately and for whole
group together. Our study showed that investigated SNP was associated with test
daily gain, average daily gain, age at slaughter and number of days in experiment
(P ≤ 0.05 or P ≤ 0.01) in Polish Large White, Puławska and whole group. Among
carcass traits MYH7 variants influenced mean backfat thickness from 5 measurements
(P ≤ 0.05) in the same groups. We also noticed some associations for
slaughter efficiency, weight of loin without backfat and skin, loin eye area and
meat percentage (P ≤ 0.05 or P ≤ 0.01) but results were not consistent among
breeds and whole group. Obtained results indicate that MYH7 SNP could be
used as a genetic marker for improvement of some growth traits and backfat
thickness in pigs.
REFERENCES (26)
1.
Aken B.L., Ayling S., Barrell D. et al., 2016. The Ensembl gene annotation system. Database (Oxford) 2016, baw093, https://doi.org/10.1093/databa....
2.
Bartz M., Kociucka B., Mankowska M., Switonski M., Szydlowski M., 2014. Transcript level of the porcine ME1 gene is affected by SNP in its 3’UTR, which is also associated with subcutaneous fat thickness. J. Anim. Breed. Genet. 131, 271–278, https://doi.org/10.1111/jbg.12....
3.
Behringer D., Zimmermann H., Ziegenhagen B., Liepelt S., 2015. Differential gene expression reveals candidate genes for drought stress response in Abies alba (Pinaceae). PLoS ONE 10, e0124564, https://doi.org/10.1371/journa....
4.
Bikandi J., San Millán R., Rementeria A., Garaizar J., 2004. In silico analysis of complete bacterial genomes: PCR, AFLP-PCR and endonuclease restriction. Bioinformatics 20, 798–799, https://doi.org/10.1093/bioinf....
5.
Callis T.E., Pandya K., Seok H.Y. et al., 2009. MicroRNA-208a is a regulator of cardiac hypertrophy and conduction in mice. J. Clin. Invest. 119, 2772–2786, https://doi.org/10.1172/JCI361....
6.
Fernandez-Rodriguez A., Munoz M., Fernandez A., Pena R.N., Tomas A., Noguera J.L., Ovilo C., Fernandez A.I., 2011. Differential gene expression in ovaries of pregnant pigs with high and low prolificacy levels and identification of candidate genes for litter size. Biol. Reprod. 84, 299–307, https://doi.org/10.1095/biolre....
7.
Horak M., Novak J., Bienertova-Vasku J., 2016. Muscle-specific microRNAs in skeletal muscle development. Dev. Biol. 410, 1–13, https://doi.org/10.1016/j.ydbi....
8.
Kim J.M., Lim K.S., Hong J.S., Kang J.H., Lee Y.S., Hong K.C., 2015. A polymorphism in the porcine miR-208b is associated with microRNA biogenesis and expressions of SOX-6 and MYH7 with effects on muscle fibre characteristics and meat quality. Anim. Genet. 46, 73–77, https://doi.org/10.1111/age.12....
9.
Komatsu Y., Sukegawa S., Yamashita M., Katsuda N., Tong B., Ohta T., Kose H., Yamada T., 2016. Identification of genes showing differential expression profile associated with growth rate in skeletal muscle tissue of Landrace weanling pig. J. Genet. 95, 341–347, https://doi.org/10.1007/s12041....
10.
Liu K., Muse SV., 2005. PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21, 2128–2129, https://doi.org/10.1093/bioinf....
11.
Luo W., Cheng D., Chen S. et al., 2012. Genome-wide association analysis of meat quality traits in a porcine Large White × Minzhu intercross population. Int. J. Biol. Sci. 8, 580–595, https://doi.org/10.7150/ijbs.3....
12.
Mahdavi V., Izumo S., Nadal-Ginard B., 1987. Developmental and hormonal regulation of sarcomeric myosin heavy chain gene family. Circ. Res. 60, 804–814, https://doi.org/10.1161/01.RES....
13.
Matoulkova E., Michalova E., Vojtesek B., Hrstka R., 2012. The role of the 3’ untranslated region in post-transcriptional regulation of protein expression in mammalian cells. RNA Biol. 9, 563–576, https://doi.org/10.4161/rna.20....
14.
Park H.-B., Han S.-H., Yoo C.-K., Lee J.-B., Kim J.-H., Baek K.-S., Son J.-K., Shin S.-M., Lim H.-T., Cho I.-C., 2017. Genome scan linkage analysis identifies a major quantitative trait loci for fatty acid composition in longissimus dorsi muscle in an F2 intercross between Landrace and Korean native pigs. Asian-Australas. J. Anim. Sci. 30, 1061–1065, https://doi.org/10.5713/ajas.1....
15.
Piórkowska K., Ropka-Molik K., Oczkowicz M., Różycki M., Żukowski K., 2013. Association study of PIT1 and GHRH SNPs with economically important traits in pigs of three breeds reared in Poland. Anim. Sci. Pap. Rep. 31, 303–314
16.
Piórkowska K., Żukowski K., Ropka-Molik K., Tyra M., 2018. Detection of genetic variants between different Polish Landrace and Puławska pigs by means of RNA-seq analysis. Anim. Genet. 49, 215–225, https://doi.org/10.1111/age.12....
17.
Reddy A.M., Zheng Y., Jagadeeswaran G., Macmil S.L., Graham W.B., Roe B.A., Desilva U., Zhang W., Sunkar R., 2009. Cloning, characterization and expression analysis of porcine microRNAs. BMC Genomics 10, 65, https://doi.org/10.1186/1471-.
18.
Różycki M., Tyra M., 2009. Methodology for assessing the value of fattening and slaughter traits of pigs carried out in Pig Slaughter Performance Control Stations (SKURTCh) (in Polish). National Research Institute of Animal Production. Krakow, Poland, pp. 94–117.
19.
Stachowiak M., Nowacka-Woszuk J., Szydlowski M., Switonski M., 2013. The ACACA and SREBF1 genes are promising markers for pig carcass and performance traits, but not for fatty acid content in the longissimus dorsi muscle and adipose tissue. Meat Sci. 95, 64–71, https://doi.org/10.1016/j.meat....
20.
Stachowiak M., Szydłowski M., Flisikowski K., Flisikowska T., Bartz M., Schnieke A., Switonski M., 2014. Polymorphism in 3’ untranslated region of the pig PPARA gene influences its transcript level and is associated with adipose tissue accumulation. J. Anim. Sci. 92, 2363–2371, https://doi.org/10.2527/jas.20....
21.
Untergasser A., Cutcutache I., Koressaar T., Ye J., Faircloth B.C., Remm M., Rozen S.G., 2012. Primer3 – new capabilities and interfaces. Nucleic Acids Res. 40, e115, https://doi.org/10.1093/nar/gk....
22.
van Rooij E., Quiat D., Johnson B.A., Sutherland L.B., Qi X., Richardson J.A., Kelm R.J. Jr, Olson E.N., 2009. A family of microRNAs encoded by myosin genes governs myosin expression and muscle performance. Dev. Cell. 17, 662–673, https://doi.org/10.1016/j.devc....
23.
van Son M., Enger E.G., Grove H., Ros-Freixedes R., Kent M.P., Lien S., Grindflek E., 2017. Genome-wide association study confirm major QTL for backfat fatty acid composition on SSC14 in Duroc pigs. BMC Genomics 18, 369, https://doi.org/10.1186/s12864....
24.
Zang L., Wang Y., Sun B., Zhang X., Yang C., Kang L., Zhao Z., Jiang Y., 2016. Identification of a 13 bp indel polymorphism in the 3’-UTR of DGAT2 gene associated with backfat thickness and lean percentage in pigs. Gene 576, 729–733, https://doi.org/10.1016/j.gene....
25.
Zhu M., Zhao S., 2007. Candidate gene identification approach: progress and challenges. Int. J. Biol. Sci. 3, 420–427, https://doi.org/10.7150/ijbs.3....
26.
Żak G., Tyra M., Różycki M., 2009. Meatiness and fatness traits of Polish Large White and Polish Landrace pigs differing in fattening traits. Ann. Anim. Sci. 9, 299–306.
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