CC-BY 4.0

Rumen microbial fermentation, protozoan abundance and boron availability in yearling rams fed diets with different boron concentrations

O. Sizmaz 1  ,  
B.H. Koksal 2,  
University of Ankara, Faculty of Veterinary Medicine, Department for Animal Nutrition and Nutritional Diseases, 06110 Ankara, Turkey
University of Adnan Menderes, Faculty of Veterinary Medicine, Department for Animal Nutrition and Nutritional Diseases, 09016 Aydın, Turkey
J. Anim. Feed Sci. 2017;26(1):59–64
Publish date: 2017-03-21
The objective of the in vivo study was to evaluate whether feeding graded levels of boron affect rumen microbial fermentation including pH, ammonia, volatile fatty acids and protozoa counts. In the experiment 4 Merino rams in a 4 × 4 Latin square design fed forage and concentrate with boric acid (0, 200, 300 and 400 mg · kg−1 in control, B1, B2 and B3 group, respectively) were used. Each experimental period lasted 14 days, with 12 first days of diet adaptation. In comparison with control diet, boron supplementation did not increased the total volatile fatty acid concentration before and 3 h after feeding. However in both time points, acetate content was higher in B1 and B2 than in control and B3 groups, whereas propionate content was lower in all boron-supplemented groups. The iso-butyrate, n-butyrate and iso-valerate levels were influenced only 3 h after feeding. The n-valerate content was lower in B1 and B2 than in control and B3 groups. Protozoan abundance in the rumen fluid was significantly higher in animals fed B3 and control diets both before and after feeding. The boron content in rumen fluid was increased in boron-supplemented groups to average value 7.32 ppm, but dose effect was not observed. The obtained results showed that dietary boron supplementation had a dose-dependent influence on rumen microbial fermentation and protozoan abundance in yearling rams. However, the boron concentration in rumen fluid did not increased simultaneously with increased dose in a diet. Further studies are needed to estimate the most recommended dose of boron in the ruminant diets and to better understand the boron role in the processes occurring in the rumen.
O. Sizmaz   
University of Ankara, Faculty of Veterinary Medicine, Department for Animal Nutrition and Nutritional Diseases, 06110 Ankara, Turkey
1. Allen J.D., Gawthornet J.W., 1987. Involvement of the solid phase of rumen digesta in the interaction between copper, molybdenum and sulphur in sheep. Br. J. Nutr. 58, 265–276, https://doi.org/10.1079/BJN19870094
2. AOAC International, 1995. Official Methods of Analysis of AOAC International. 16th Edition. Arlington, VA (USA)
3. Çinar M., Küçükyilmaz K., Bozkurt M., Çatli A.U., Bintaş E., Akşit H., Konak R., Yamaner Ç., Seyrek K., 2015. Effects of dietary boron and phytase supplementation on growth performance and mineral profile of broiler chickens fed on diets adequate or deficient in calcium and phosphorus. Br. Poult. Sci. 56, 576–589, https://doi.org/10.1080/00071668.2015.1079699
4. Deckardt K., Metzler-Zebeli B.U., Zebeli Q., 2016. Processing barley grain with lactic and tannic acid ameliorates rumen microbial fermentation and degradation of dietary fibre in vitro. J. Sci Food Agric. 96, 223–231,https://doi.org/10.1002/jsfa.7085
5. Devirian T.A, Volpe S.L., 2003. The physiological effects of dietary boron. Crit. Rev. Food Sci. Nutr. 43, 219–231, https://doi.org/10.1080/10408690390826491
6. Hunt C.D., Herbel S.L., Nielsen F.H., 1997. Metabolic responses of postmenopausal women to supplemental dietary boron and aluminum during usual and low magnesium intake: boron, calcium and magnesium absorption and retention and blood mineral concentrations. Am. J. Clin. Nutr. 65, 803–813
7. Kabu M., Uyarlar C., 2015. The effects of borax on milk yield and selected metabolic parameters in Austrian Simmental (Fleckvieh) cows. Vet. Med. 60, 175–180, https://doi.org/10.17221/8104-VETMED
8. Küçükyilmaz K., Erkek R., Bozkurt M., 2014. The effects of boron supplementation of layer diets varying in calcium and phosphorus concentrations on performance, egg quality, bone strength and mineral constituents of serum, bone and faeces. Br. Poult. Sci. 55, 804–816, https://doi.org/10.1080/00071668.2014.975782
9. Lu C.D., Kawas J.R., Mahgoub O.G., 2008. Recent advancements in fiber digestion and utilization in goats. Trop. Subtrop. Agroecosyst. 9, 65–72
10. Mao S.Y., Zhang G., Zhu W.Y., 2008. Effect of disodium fumarate on ruminal metabolism and rumen bacterial communities as revealed by denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA. Anim. Feed Sci. Technol. 140, 293–306, https://doi.org/10.1016/j.anifeedsci.2007.04.001
11. Mathieu F., Jouany J.P., Sénaud J., Bohatier J., Bertin G., Mercier M., 1996. The effect of Saccharomyces cerevisiae and Aspergillus oryzae on fermentations in the rumen of faunated and defaunated sheep; protozoal and probiotic interactions. Reprod. Nutr. Dev. 6, 271–287, https://doi.org/10.1051/rnd:19960305
12. McDonald P., Edwards R.A., Greenhalgh J.F.D., Morgan C.A., Sinclair L.A., Wilkinson G., 2010. Animal Nutrition. 7th Edition, Pearson, Oxford (UK)
13. Miltko R., Rozbicka-Wieczorek J.A., Więsyk E., Czauderna M., 2016. The influence of different chemical forms of selenium added to the diet including carnosic acid, fish oil and rapeseed oil on the formation of volatile fatty acids and methane in the rumen, and fatty acid profiles in the rumen content and muscles of lambs. Acta Vet. Beogr. 66, 373–391, https://doi.org/10.1515/acve-2016-0032
14. Ogimoto K., Imai S., 1981. Atlas of Rumen Microbiology. Japan Scientific Societies Press, Tokyo (Japan), p. 158
15. Ørskov E.R, Ryle M., 1990. Energy Nutrition in Ruminants. Elsevier Science Publishers Ltd., Essex (UK)
16. Rozbicka-Wieczorek A.J., Czauderna M., Więsyk E., Radzik-Rant A., 2016. Selenium species in diet containing carnosic acid, fish and rapeseed oils affect fatty acid profiles in lamb muscles. J. Anim. Feed Sci. 25, 216–225, https://doi.org/10.22358/jafs/65555/2016
17. Serbester U., 2013. Determination of boron level in feeds used in cattle nutrition in regions of central Anatolia and mediterranean of Turkey. KSÜ Doğa Bil. Derg. 16, 25–27
18. Sizmaz Ö., Yildiz G., 2014. Effects of dietary boric acid and ascorbic acid supplementation on performance, some blood and bone parameters in broilers. Kafkas Univ. Vet. Fak. Derg. 20, 55–61
19. Spears J.W., 2003. Trace mineral bioavailability in ruminants. J. Nutr. 133, Suppl. 1, 1506S–1509S
20. Tanaka M., Fujiwara T., 2008. Physiological roles and transport mechanisms of boron: perspectives from plants. Pflüg. Arch. Eur. J. Physiol. 456, 671–677, https://doi.org/10.1007/s00424-007-0370-8
21. TSE, 1991. Animal Feeds. Metabolic Energy Determination (Chemical Method). Turkish Standards Institute (TSE), Publication No. 9610, Ankara (Turkey)
22. WHO, 1998. International Programme on Chemical Safety. Environmental Health Criteria 204: Boron. Geneva (Switzerland), pp. 201
23. Vanatta L.E., Coleman D.E., Slingsby R.W., 1999. Low-level calibration study for a new ion chromatographic column to determine borate in deionized water. J. Chrom. A 850, 107–117, https://doi.org/10.1016/S0021-9673(99)00263-0
24. Yildiz G., Köksal B.H., Sizmaz Ö., 2013. Influence of dietary boric acid and liquid humate inclusion on bone characteristics, growth performance and carcass traits in broiler chickens. Arch. Geflügelk. 77, 260–265