Effects of boron supplementation alone or in combination with different vitamin D3 levels on laying performance, eggshell quality, and mineral content and fatty acid composition of egg yolk in laying hens
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Ankara University, Faculty of Veterinary Medicine, Department of Animal Nutrition and Nutritional Disease, 06110 Ankara, Turkey
Adnan Menderes University, Faculty of Veterinary Medicine, Department of Animal Nutrition and Nutritional Disease, 09016 Aydın, Turkey
Van Yüzüncü Yıl University, Agriculture Faculty, Department of Animal Science, 65080 Van, Turkey
Publication date: 2021-08-27
Corresponding author
Ö. Simaz   

Ankara University, Faculty of Veterinary Medicine, Department of Animal Nutrition and Nutritional Disease, 06110 Ankara, Turkey
J. Anim. Feed Sci. 2021;30(3):288-294
This study aimed to evaluate the effects of dietary boric acid with vitamin D3 on laying performance, eggshell quality, and mineral content and fatty acid composition of egg yolk in hens. In the experiment, 160 late-phase laying hens were equally divided into four groups, 40 birds each (10 replicates of 4 hens each). The study included a control group and three treatment groups. In the control group, there was no additive, while the experimental groups contained: 1) vitamin D3 3000 IU/kg + 120 ppm boric acid, HDB; 2) vitamin D3 300 IU/kg + 120 ppm boric acid, LDB; and 3) 120 ppm boric acid, B; respectively. After 12 weeks of feeding, the egg mass was significantly reduced in HDB group in comparison to the control and feed efficiency was increased in HDB group in comparison to the control and B groups (P < 0.05). No significant differences were observed in eggshell quality among groups (P > 0.05). Egg yolk cholesterol content was decreased in LDB group in comparison to B group (P < 0.05). The concentration of C18:3 fatty acid was the highest (P < 0.05) in B group. In comparison to the control group, the C16:0 fatty acid concentration was lower in B group (P < 0.05). So, the combination of boric acid (120 ppm) and a high dose of vitamin D3 (3000 IU/kg) is not recommended in laying hen feeding as it negatively influences laying performance parameters. On the other hand, although the supplementation of 120 ppm boric acid without additional vitamin D3 increases egg yolk omega-3 fatty acid content, the cholesterol-reducing effect (but without fatty acid profile change) may be obtained only when boric acid is added simultaneously with a low level of vitamin D3 (300 IU/kg), which can be used to produce functional food.
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The authors declare that there is no conflict of interest.
Ackman R.G., 1989. Absorption of omega-3 fatty acids. Nutrition 5, 251–253.
Aguillón-Páez Y.J., Romero L.A., Diaz G.J., 2020. Effect of full-fat sunflower or flaxseed seeds dietary inclusion on performance, egg yolk fatty acid profile and egg quality in laying hens. Anim. Nutr. 6, 179–184,
AOAC International, 2000. Official Methods of Analysis of AOAC International. 17th Edition. Gaithersburg, MD (USA).
Basoglu A., Baspinar N., Tenori L., Vignoli A., Gulersoy E., 2017. Effects of boron supplementation on peripartum dairy cows’ health. Biol. Trace Elem. Res. 179, 218–225,
Białek M., Czauderna M., Krajewska K.A., Przybylski W., 2019. Selected physiological effects of boron compounds for animals and humans. A review. J. Anim. Feed. Sci. 28, 307–320,
Grossu D.V., Criste R.D., Scorei R., Duca R., Ciurascu C., 2005. Effect of the supplemental PROLINBOR, boron and linolenic acid-enriched protein concentrate, added to layer diets on egg quality. In: Proceedings of the XVII European Symposium on the Quality of Poultry Meat and XI European Symposium on the Quality of Eggs and Egg Products. Doorwerth (Netherlands). World’s Poultry Science Association (WPSA). Beekbergen (Netherlands), pp. 113–118.
Hayat Z., Cherian G., Pasha T.N., Khattak F.M., Jabbar M.A., 2009. Effect of feeding flax and two types of antioxidants on egg production, egg quality, and lipid composition of eggs. J. Appl. Poult. Res. 18, 541–551,
Hegsted M., Keenan M.J., Siver F., Wozniak P., 1991. Effect of boron on vitamin D deficient rats. Biol. Trace Elem. Res. 28, 243–255,
Hunt C.D., 1994. The biochemical effects of physiologic amounts of dietary boron in animal nutrition models. Environ. Health Perspect. 102, Suppl. 7, 35–43,
Hunt C.D., Nielsen F.H., 1981. Interaction between boron and cholecalciferol in the chick. In: J. Gawthorne, J.McC. Howell, C.L. White (Editors). Trace Element Metabolism in Man and Animals. Proceedings of the Fourth International Symposium on Trace Element Metabolism in Man and Animals (TEMA-4). Perth (Australia). Australian Academy of Science. Canberra (Australia), pp. 597–600,
Kaya H., Macit M., 2018. The effect of boron (orthoboric acid) supplementation into diets of laying hens on some egg yolk parameters during late laying period. Turkish J. Agric. - Food Sci. Technol. 6, 278–284,
Kılıç A.M., Kahraman E., Kılıç Ö., 2018. Place of Turkey in the world boron production and importance. In: Proceedings of International Congress on Engineering and Architecture (ENAR-2018). Alanya (Turkey), pp. 1598–1607.
Koksal B.H., Yildiz G., Sizmaz O., 2012. Effects of boric acid and humate supplementation on performance and egg quality parameters of laying hens. Braz. J. Poult. Sci. 14, 283–289,
Kurtoglu V., Kurtoglu F., Coskun B., Seker E., Balevi T., Çetingul I.S., 2002. Effects of boron supplementation on performance and some serum biochemical parameters in laying hens. Revue Méd. Vét. 153, 823–828.
Küçükyılmaz K., Erkek R., 2012. The effects of boron supplementation to layer hen diet containing different levels of calcium and phosphorus on mineral content of egg (in Turkish: Farklı düzeylerde kalsiyum ve fosfor içeren yumurta tavuğu karma yemlerine bor ilavesinin yumurta mineral içerikleri üzerine etkileri). Adnan Menderes Üniv. Ziraat Fak. Derg. 9, 33–38.
Manor M.L., Derksen T.J., Magnuson A.D., Raza F., Lei X.G., 2019. Inclusion of dietary defatted microalgae dose-dependently enriches ω-3 fatty acids in egg yolk and tissues of laying hens. J. Nutr. 149, 942–950,
Metcalfe L.D., Schmitz A.A., 1961. The rapid preparation of fatty acid esters for gas chromatographic analysis. Anal. Chem. 33, 363–364,
Mizrak C., 2008. The effect of different levels and forms of boron supplementation in breeder laying hen diets on performance, bone development, egg quality and some blood parameters. PhD Thesis. Ankara University Institute of Science. Ankara (Turkey).
Nielsen F.H., 2002. Does boron have an essential function similar to an omega-3 fatty acid function? In: M. Anke, R. Muller, U. Schafer, M. Stoeppler (Editors). Macro and Trace Elements (Meeting Proceedings). Friedrich Schiller University, Jena (Germany). Schubert-Verlag. Leipzig (Germany), pp. 1238–1250.
Nielsen F.H., Eckhert C.D., 2020. Boron. Adv. Nutr. 11, 461–462,
NRC (National Research Council), 1984. Nutrients Requirements of Poultry. 8th Revised Edition. The National Academies Press. Washington, DC (USA),
NRC (National Research Council), 1994. Nutrient Requirements of Poultry. 9th Revised Edition. The National Academies Press. Washington, DC (USA),
Olgun O., Cufadar Y., Yildiz A.O., 2009. Effects of boron supplementation fed with low calcium to diet on performance and egg quality in molted laying hens. J. Anim. Vet. Adv. 8, 650–654.
Scorei R.I., Rotaru P., 2011. Calcium fructoborate – potential antiinflammatory agent. Biol. Trace Elem. Res. 143, 1223–1238,
Sizmaz O., Yildiz G., 2016. Influence of dietary boric acid and ascorbic acid on performance, egg traits, cholesterol and bone parameters of laying hens. Ankara Üniv. Vet. Fak. Derg. 63, 151–156,
Sizmaz Ö., Yildiz G., Köksal B.H., 2014. Effects of single or combined dietary supplementation of boric acid and plant extract mixture on egg production, egg quality and blood cholesterolemia in laying hens. Kafkas Univ. Vet. Fak. Derg. 20, 599–604,
Sokmen N., Buyukakinci B.Y., 2018. The usage of boron/boron compounds in the textile industry and its situation in Turkey. In: P. Hájek, O. Vít (Editors). CBU International Conference Proceedings, Vol. 6. CBU International Conference on Innovations in Science and Education. Prague (Czech Republic). CBU Research Institute s.r.o. Prague (Czech Republic), pp. 1158–1165,
Yao L., Wang T., Persia M., Horst R.L., Higgins M., 2013. Effects of vitamin D3-enriched diet on egg yolk vitamin D3 content and yolk quality. J. Food Sci. 78, C178–C183,
Zhang Y.N., Zhang H.J., Wang J., Yue H.Y., Qi X.L., Wu S.G., Qi G.H., 2017. Effect of dietary supplementation of organic or inorganic zinc on carbonic anhydrase activity in eggshell formation and quality of aged laying hens. Poult. Sci. 96, 2176–2183,
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