ORIGINAL PAPER
Factors affecting the production of potential transgenic pigs by DNA microinjection; a six-year retrospective study
J. Jura 1,   Z. Smorąg 1  
,   R. Słomski 2,   D. Lipiński 2,   B. Gajda 1
 
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1
National Research Institute of Animal Production, 32-083 Balice, Poland
2
The August Cieszkowski Agricultural University, Department of Biochemistry and Biotechnology, Wołyńska 35, 60-637 Poznań, Poland
CORRESPONDING AUTHOR
Z. Smorąg   

National Research Institute of Animal Production, 32-083 Balice, Poland
Publication date: 2007-11-21
 
J. Anim. Feed Sci. 2007;16(4):636–645
 
KEYWORDS
ABSTRACT
Transgenesis is a complex process. Among the most important factors affecting the production of potential transgenic pigs using standard DNA microinjection into zygote pronuclei are the type of promoter used to express the gene construct and the method of zygote transfer used for transformation. The transformation of zygotes using different gene constructs showed that the promoter type influenced the developmental capability of the transformed zygotes, and this affected the percentage of born piglets. The transfer of transformed zygotes into a single oviduct resulted in a higher percentage of potential transgenic piglets born, this figure was almost doubled compared with transfer into both oviducts. Recently, research into the production of transgenic pigs has shown that factors connected with reproduction physiology and embryological factors such as the number of zygotes transferred after transformation and the season of the year have a significant influence but are not critical to the efficiency of production of transgenic pigs. The best results were obtained when less than 20 transformed zygotes per recipient were transplanted and the autumn season is the time when the environmental factors are optimal for production of potential transgenic pigs
 
CITATIONS (4):
1.
Nanoparticles as a Tool for Transfection and Transgenesis – a Review
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Annals of Animal Science
 
2.
A Site-Specific Recombinase-Based Method to Produce Antibiotic Selectable Marker Free Transgenic Cattle
Yuan Yu, Yongsheng Wang, Qi Tong, Xu Liu, Feng Su, Fusheng Quan, Zekun Guo, Yong Zhang, Xiuhcun (Cindy) Tian
PLoS ONE
 
3.
The production of UL16-binding protein 1 targeted pigs using CRISPR technology
Zeyland Joanna, Hryhorowicz Magdalena, Nowak-Terpiłowska Agnieszka, Jura Jacek, Słomski Ryszard, Smorąg Zdzisław, Gajda Barbara, Lipiński Daniel
3 Biotech
 
4.
Production of ULBP1-KO pigs with human CD55 expression using CRISPR technology
Agnieszka Nowak-Terpiłowska, Daniel Lipiński, Magdalena Hryhorowicz, Wojciech Juzwa, Jacek Jura, Ryszard Słomski, Natalia Mazurkiewicz, Barbara Gawrońska, Joanna Zeyland
Journal of Applied Animal Research
 
ISSN:1230-1388