ORIGINAL PAPER
Comparison of time-resolved fluoroimmunoassay for clenbuterol residues in pig liver with enzyme-linked immunosorbent assay
L. Bi. Wang 1  
,   X. Q. Li 2,   Ti M. Huo 2,   Ke Li 2,   Ch.-L. Xu 2
 
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1
TianJin Academy of Inspection and Quarantine, 300457 TianJin, P.R. China
2
School of Food Science and Technology, Southern Yangtze University, 1800 Lihu Road, Wuxi, Jiangsu Province, 214122 P.R. China
CORRESPONDING AUTHOR
L. Bi. Wang   

TianJin Academy of Inspection and Quarantine, 300457 TianJin, P.R. China
Publication date: 2007-11-21
 
J. Anim. Feed Sci. 2007;16(4):646–653
 
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ABSTRACT
A time-resolved fluoroimmunoassay (TR-FIA) for determination of clenbuterol (CLB) in pig liver was developed. The limit of detection (LOD) was 0.02 ng g-1 and limit of quantification (LOQ), 0.08 ng g-1. Recoveries ranged from 89.3 to 117.9% for pig liver at spiked levels of 0.1-5 ng g-1. The results obtained by the TR-FIA and enzyme-linked immunosorbent assay (ELISA) showed a good correlation. The established TR-FIA was applied for screening pig liver from the local market and confirmed by gas chromatography-mass spectrometry (GC-MS). This proposed technique could be used for routine screening for drug residues.
ISSN:1230-1388