ORIGINAL PAPER
Comparison of time-resolved fluoroimmunoassay
for clenbuterol residues in pig liver with
enzyme-linked immunosorbent assay
| 1 | TianJin Academy of Inspection and Quarantine,
300457 TianJin, P.R. China |
| 2 | School of Food Science and Technology, Southern Yangtze University,
1800 Lihu Road, Wuxi, Jiangsu Province, 214122 P.R. China |
CORRESPONDING AUTHOR
Publication date: 2007-11-21
J. Anim. Feed Sci. 2007;16(4):646–653
KEYWORDS
ABSTRACT
A time-resolved fluoroimmunoassay (TR-FIA) for determination of clenbuterol (CLB) in pig
liver was developed. The limit of detection (LOD) was 0.02 ng g-1 and limit of quantification (LOQ),
0.08 ng g-1. Recoveries ranged from 89.3 to 117.9% for pig liver at spiked levels of 0.1-5 ng g-1. The
results obtained by the TR-FIA and enzyme-linked immunosorbent assay (ELISA) showed a good
correlation. The established TR-FIA was applied for screening pig liver from the local market and
confirmed by gas chromatography-mass spectrometry (GC-MS). This proposed technique could be
used for routine screening for drug residues.
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