Comparison of time-resolved fluoroimmunoassay
for clenbuterol residues in pig liver with
enzyme-linked immunosorbent assay

A time-resolved fluoroimmunoassay (TR-FIA) for determination of clenbuterol (CLB) in pig liver was developed. The limit of detection (LOD) was 0.02 ng g^-1 and limit of quantification (LOQ), 0.08 ng g^-1. Recoveries ranged from 89.3 to 117.9% for pig liver at spiked levels of 0.1-5 ng g^-1. The results obtained by the TR-FIA and enzyme-linked immunosorbent assay (ELISA) showed a good correlation. The established TR-FIA was applied for screening pig liver from the local market and confirmed by gas chromatography-mass spectrometry (GC-MS). This proposed technique could be used for routine screening for drug residues.

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1230-1388

Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.