SHORT COMMUNICATION
Effect of thymosin β on maturation of pig oocytes and quality of in vitro produced embryos
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National Research Institute of Animal Production, Department of Reproductive Biotechnology and Cryopreservation, 32-083 Balice/Kraków, Poland
 
 
Publication date: 2020-11-18
 
 
Corresponding author
K. Poniedziałek-Kempny   

National Research Institute of Animal Production Department of Reproductive Biotechnology and Cryopreservation, 32-083 Balice/Krakow, Poland
 
 
J. Anim. Feed Sci. 2020;29(4):358-361
 
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ABSTRACT
Thymosin (TMS) is a peptide hormone that has regenerative qualities. To date, the regenerative qualities of thymosin have been tested only on somatic cells and bovine oocytes. The objective of this study was to determine the effect of thymosin on the in vitro maturation of pig oocytes and on in vitro fertilization (IVF), which would be used to assess the quality of obtained embryos. Pig oocytes (n = 463) were matured in vitro to the Met II stage in medium supplemented with TMS. After IVF the zygotes were cultured in vitro until they reached the blastocyst stage. The number of cells and the level of nuclear DNA fragmentation were evaluated. The addition of TMS to the maturation medium resulted in a significant increase in the percentage of competent oocytes that, after fertilization, developed to the blastocyst stage. Moreover, supplementing the maturation medium with TMS had a positive effect on the quality of obtained blastocysts by limiting the occurrence of apoptosis.
 
REFERENCES (15)
1.
Chen L., Zhang J.J., Zhang X., Liu X., Zhao S., Huo L.J., Zhou J., Miao Y.L., 2020. Melatonin protects against defects induced by malathion during porcine oocyte maturation. J. Cell. Physiol. 235, 2836–2846, https://doi.org/10.1002/jcp.29....
 
2.
Gajda B., Romek M., Grad I., Krzysztofowicz E., Bryla M., Smorag Z., 2011. Lipid content and cryotolerance of porcine embryos cultured with phenazine ethosulfate. CryLetters 32, 349–357, https://doi.org/10.1071/RDv23n....
 
3.
Gajda B., Poniedzialek-Kempny K., Rajska I., Smorąg Z., 2017. Effect of thymosin on survival of vitrified porcine oocytes. Proc. 7th Workshop of the Society for Biology of Reproduction, Olsztyn, pp. 104.
 
4.
Goldstein A.L., Slater F.D., White A., 1966. Preparation, assay, and partial purification of a thymic lymphocytopoietic factor (thymosin). Proceedings of the National Academy of Sciences of the United States of America, 56, 1010–1017, https://doi.org/10.1073/pnas.5....
 
5.
Hinkel R., Ball H.L., DiMaio J.M. et al., 2015. C-terminal variable AGES domain of thymosin β4: the molecule’s primary contribution in support of post-ischemic cardiac function. J. Mol. Cell. Cardiol. 87, 113–125, https://doi.org/10.1016/j.yjmc....
 
6.
Kumar S., Gupta S., 2011. Thymosin beta 4 prevents oxidative stress by targeting antioxidant and anti-apoptotic genes in cardiac fibroblasts. PLoS One 11–13, https://doi.org/10.1371/journa....
 
7.
Li J., Liu CH.H., Wang F.S., 2010. Thymosin alpha 1: Biological activities, applications and genetic engineering production. Peptides 31, 2151–2158, https://doi.org/10.1016/j.pept....
 
8.
Nguyen T.V., Tanihara F., Do L., Sato Y., Taniguchi M., Takagi M., Van Nguyen T., Otoi T., 2017. Chlorogenic acid supplementation during in vitro maturation improves maturation, fertilization and developmental competence of porcine oocytes. Reprod. Dom. Anim. 52, 969–975, https://doi.org/10.1111/rda.13....
 
9.
Petters R.M., Wells K.D., 1993. Culture of pig embryos. J. Reprod. Fertil. 48, 61–73.
 
10.
Poniedziałek-Kempny K., Gajda B., Rajska I., Gajda L., Smorąg Z., 2020. Piglets produced by transfer of embryos obtained by in vitro fertilization of oocytes matured in vitro with thymosin: a case report. Med. Wet. 76, 181–185, https://doi.org/10.21521/mw.63....
 
11.
Rando O.J., 2000. Searching for a function for nuclear actin. Trends. Cell. Biol. 10, 92–97, https://doi.org/10.1016/S0962-....
 
12.
Skowronek O., Zdebska N., Opiela J., 2016. The impact of thymosin β4 on bovine oocytes meiotic maturation and fragmentation DNA. Proc. 4th Winter Workshop of the Society for Biology of Reproduction. Zakopane, Poland, pp. 25.
 
13.
Trzcińska M., Bryła M., Smorąg Z., 2008. Effect of liquid storage on membrane integrity and mitochondrial activity: a new diagnostic method of evaluating boar sperm quality. J. Anim. Feed. Sci. 17, 372–380, https://doi.org/10.22358/jafs/....
 
14.
Tseng B.S., Zhao P., Pattison J.S., Gordon S.E., Granchelli J.A., Madsen R.W., Folk L.C., Hoffman E.P., Booth F.W., 2002. Regenerated mdx mouse skeletal muscle shows differential mRNA expression. J. Appl. Physiol. 93, 537–545, https://doi.org/10.1152/japplp....
 
15.
Wierzchoś-Hilczer A., 2017. Thymosin β4 structure - multiple biological functions and potential therapeutic applications. Wiad. Zootech. (in Polish). R. LV 4, 30-36.
 
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