ORIGINAL PAPER
Effect of the Ovsynch protocol on ovarian changes
and pregnancy rates after first insemination in lactating cows
with or without puerperal metritis
1
Lithuanian University of Health Sciences, Faculty of Veterinary, Veterinary Academy, Large Animals Clinic,
LT-47181 Kaunas, Lithuania
2
Lithuanian University of Health Sciences, Faculty of Animal Sciences, Veterinary Academy, Animal Breeding Department,
LT-47181 Kaunas, Lithuania
3
Lithuanian University of Health Sciences, Faculty of Veterinary, Veterinary Academy, Department of Veterinary Pathobiology,
LT-47181 Kaunas, Lithuania
4
Lithuanian University of Health Sciences, Faculty of Veterinary, Veterinary Academy, Dr. L. Kriaučeliūnas Small Animal Clinic,
LT-47181 Kaunas, Lithuania
Publication date: 2022-09-09
Corresponding author
V. Juodžentis
Large Animals Clinic, Faculty of Veterinary, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės str. 18, LT-47181, Kaunas, Lithuania.
Large Animals Clinic, Faculty of Veterinary, Veterinary Academy, Lithuanian University of Health Sciences, Tilžės str. 18, LT-47181, Kaunas, Lithuania.
J. Anim. Feed Sci. 2022;31(4):310-317
KEYWORDS
TOPICS
ABSTRACT
The aim of this study was to determine how puerperal metritis
affects pregnancy rates and ovarian changes in dairy cows synchronised
with the Ovsynch protocol. The study included 72 cows, which were divided
into two groups. Cows treated for puerperal metritis were defined as
Group M (n = 34), and those without signs of puerperal metritis as Group H
(n = 38). Cows were synchronised for the first artificial insemination (AI) on day
49 ± 3 postpartum using a modified Ovsynch protocol. The number of cows
that ovulated after the first injection of gonadotropin-releasing hormone (G1)
was similar between Group H and Group M (36.84 and 23.53%, respectively;
P > 0.05). At G1, the size of dominant follicles was smaller in healthy nonovulated subgroup (HNO) compared to metritic an-ovulated cows (MNO)
(P < 0.05). Furthermore, the volume of corpus luteum was lower in the subgroup
of metritic ovulated cows (MO) compared to healthy ovulated cows (HO)
(P < 0.05). At the time of prostaglandin F2α treatment, progesterone (P4) levels
in subgroups HO and MO were > 1 ng/ml, and they were different compared
to subgroups HNO and MNO (P < 0.05). The study showed that at the time of
the second injection of gonadotropin-releasing hormone (G2), follicle size was
larger in Subgroup MO compared to Subgroup HO (P < 0.05). In conclusion, at
the time of synchronisation, P4 concentration and luteal volume size was lower
in metritic cows compared to healthy counterparts. However, pregnancy and
embryo mortality rates were similar between metritic and healthy cows.
ACKNOWLEDGEMENTS
This study was supported by the Science Foundation of the Lithuanian University of Health Sciences (LUHS).
CONFLICT OF INTEREST
The Authors declare that there is no conflict of
interest.
METADATA IN OTHER LANGUAGES:
Chinese
Ovsynch方案对罹患产后子宫炎和健康泌乳奶牛首次授精后
的卵巢变化和妊娠率的影响
摘要: 本研究目的是确定采用Ovsynch方案进行同期排卵处理后产后子宫炎如何影响奶牛妊
娠率和卵巢变化。72头奶牛被分成两组：罹患产后子宫炎奶牛为M组（n = 34），无产后
子宫炎奶牛为H组（n = 38）。奶牛在产后49 ± 3天采用改良的同期排卵方案（Ovsynch）进
行第一次人工授精（Artificial insemination，AI）。H组和M组第一次注射促性腺激素释放激
素（Gonadotropin-releasing hormone，G1）后奶牛排卵数无显著差异（分别为36.84%和23.53%
，P > 0.05）。注射G1后，健康未排卵组（Healthy non-ovulated subgroup，HNO）奶牛优势
卵泡的体积显著小于罹患子宫炎的排卵期奶牛（Metritic an-ovulated，MNO）（P < 0.05）。
此外，罹患子宫炎的已排卵奶牛（Metritic ovulated cows，MO）组的黄体体积显著低于
健康已排卵奶牛（Healthy ovulated cows，HO）（P < 0.05）。注射前列腺素（Prostaglandin
F2
α，PGF2α）后，HO和MO组奶牛的孕酮（Progesterone，P4）水平均大于1 ng/ml，与HNO和
MNO组比较差异显著（P < 0.05）。结果表明，第二次注射G2时，MO组卵泡体积显著大于
HO组（P < 0.05）。研究结果表明，在同期排卵处理时，与健康奶牛相比，罹患子宫炎奶
牛的P4浓度和黄体容积较低。然而，罹患子宫炎奶牛和健康奶牛的妊娠和胚胎死亡率相
似。
REFERENCES (43)
1.
Armengol R., Fraile L., 2015. Comparison of two treatment strategies for cows with metritis in high-risk lactating dairy cows. Theriogenology 83, 1344–1351, https://doi.org/10.1016/j.ther...
2.
Barletta R.V., Carvalho P.D., Santos V.G., Melo L.F., Consentini C.E., Netto A.S., Fricke P.M., 2018. Effect of dose and timing of prostaglandin F2α treatments during a Resynch protocol on luteal regression and fertility to timed artificial insemination in lactating Holstein cows. J. Dairy Sci. 101, 1730–1736, https://doi.org/10.3168/jds.20...
3.
Bello N.M., Pursley J.R, 2007. Strategies to maximize ovulation to first GnRH of Ovsynch in lactating dairy cows. J. Dairy Sci. 90, 326–327, https://doi.org/10.3168/jds.S0...
4.
Bello N.M., Steibel J.P., Pursley J.R., 2006. Optimizing ovulation to first GnRH improved outcomes to each hormonal injection of ovsynch in lactating dairy cows. J. Dairy Sci. 89, 3413–3424
5.
Benzaquen M.E., Risco C.A., Archbald L.F., Melendez P., Thatcher M.-J., Thatcher W.W., 2007. Rectal temperature, calving-related factors, and the incidence of puerperal metritis in postpartum dairy cows. J. Dairy Sci. 90, 2804–2814, https://doi.org/10.3168/jds.20...
6.
Bisinotto R.S., Castro L.O., Pansani M.B. et al., 2015. Progesterone supplementation to lactating dairy cows without a corpus luteum at initiation of the Ovsynch protocol. J. Dairy Sci. 98, 2515–2528, https://doi.org/10.3168/jds.20...
7.
Bisinotto R.S., Ribeiro E.S., Lima F.S., Martinez N., Greco L.F., Barbosa L.F., Bueno P.P., Scagion L.F., Thatcher W.W., Santos J.E.P., 2013. Targeted progesterone supplementation improves fertility in lactating dairy cows without a corpus luteum at the initiation of the timed artificial insemination protocol. J. Dairy Sci. 96, 2214–2225, https://doi.org/10.3168/jds.20...
8.
Brusveen D.J., Souza A.H., Wiltbank M.C., 2009. Effects of additional prostaglandin F2α and estradiol-17β during Ovsynch in lactating dairy cows. J. Dairy Sci. 92, 1412–1422, https://doi.org/10.3168/jds.20...
9.
Caraviello D.Z., Weigel K.A., Fricke P.M., Wiltbank M.C., Florent M.J., Cook N.B., Nordlund K.V., Zwald N.R., Rawson C.L., 2006. Survey of management practices on reproductive performance of dairy cattle on large US commercial farms. J. Dairy Sci. 89, 4723–4735, https://doi.org/10.3168/jds.S0...
10.
Carvalho P.D., Santos V.G., Fricke H.P., Hernandez L.L., Fricke P.M., 2019. Effect of manipulating progesterone before timed artificial insemination on reproductive and endocrine outcomes in high producing multiparous Holstein cows. J. Dairy Sci. 102, 7509–7521, https://doi.org/10.3168/jds.20...
11.
Cerri R.L., Chebel R.C., Rivera F., Narciso C.D., Oliveira R.A., Amstalden M., Baez-Sandoval G.M., Oliveira L.J., Thatcher W.W., Santos J.E.P., 2011. Concentration of progesterone during the development of the ovulatory follicle: II. Ovarian and uterine responses. J. Dairy Sci. 94, 3352–3365, https://doi.org/10.3168/jds.20...
12.
Chebel R.C., Santos J.E.P., Cerri R.L.A., Rutigliano H.M., Bruno R.G.S., 2006. Reproduction in dairy cows following progesterone insert presynchronization and resynchronization protocols. J. Dairy Sci. 89, 4205–4219, https://doi.org/10.3168/jds.S0...
13.
Chebel R.C., Santos J.E.P., Reynolds J.P., Cerri R.L., Juchem S.O., Overton M., 2004. Factors affecting conception rate after artificial insemination and pregnancy loss in lactating dairy cows. Anim. Reprod. Sci. 84, 239–255, https://doi.org/10.1016/j.anir...
14.
Chenault J.R., McAllister J.F., Chester S.T.Jr., Dame K.J., Kausche F.M., Robb E.J., 2004. Efficacy of ceftiofur hydrochloride sterile suspension administered parenterally for the treatment of acute postpartum metritis in dairy cows. J. Am. Vet. Med. Assoc. 224, 1634–1639, https://doi.org/10.2460/javma....
15.
Colazo M.G., Dourey A., Rajamahendran R., Ambrose D.J., 2013. Progesterone supplementation before timed AI increased ovulation synchrony and pregnancy per AI, and supplementation after timed AI reduced pregnancy losses in lactating dairy cows. Theriogenology 79, 833–841, https://doi.org/10.1016/j.ther...
16.
Cunha A.P., Guenther J.N., Maroney M.J., Giordano J., Nascimento A.B., Bas S., Ayres H., Wiltbank M.C., 2008. Effects of high vs low progesterone concentrations during Ovsynch on double ovulation and pregnancies per AI in high producing dairy cows. J. Dairy Sci. 91, 246
17.
Denicol A.C., Lopes G.Jr., Mendonça L.G., Rivera F.A., Guagnini F., Perez R.V., Lima J.R., Bruno R.G., Santos J.E.P., Chebel R.C., 2012. Low progesterone concentration during the development of the first follicular wave reduces pregnancy per insemination of lactating dairy cows. J. Dairy Sci. 95, 1794–1806, https://doi.org/10.3168/jds.20...
18.
Endo N., Nagai K., Tanaka T., Kamomae H., 2012. Comparison between lactating and non-lactating dairy cows on follicular growth and corpus luteum development, and endocrine patterns of ovarian steroids and luteinizing hormone in the estrous cycles. Anim. Reprod. Sci. 134, 112–118, https://doi.org/10.1016/j.anir...
19.
Esposito G., Irons P.C., Webb E.C., Chapwanya A., 2014. Interactions between negative energy balance, metabolic diseases, uterine health and immune response in transition dairy cows. Anim. Reprod Sci. 144, 60–71, https://doi.org/10.1016/j.anir...
20.
Galvão K.N., Frajblat M., Butler W.R., Brittin S.B., Guard C.L., Gilbert R.O., 2010. Effect of early postpartum ovulation on fertility in dairy cows. Reprod. Domest. Anim. 45, 207–211, https://doi. org/10.1111/j.1439-0531.2009.01517.x
21.
Galvão K.N., Sá Filho M.F., Santos J.E.P., 2007. Reducing the interval from presynchronization to initiation of timed artificial insemination improves fertility in dairy cows. J. Dairy Sci. 90, 4212–4218, https://doi.org/10.3168/jds.20...
22.
Giordano J.O., Wiltbank M.C., Guenther J.N., Pawlisch R., Bas S., Cunha A.P., Fricke P.M., 2012. Increased fertility in lactating dairy cows resynchronized with Double-Ovsynch compared with Ovsynch initiated 32 d after timed artificial insemination. J. Dairy Sci. 95, 639–653, https://doi.org/10.3168/jds.20...
23.
Giuliodori M.J., Magnasco R.P., Becu-Villalobos D., Lacau-Mengido I.M., Risco C.A., de la Sota R.L., 2013. Metritis in dairy cows: risk factors and reproductive performance. J. Dairy Sci. 96, 3621–3631, https://doi.org/10.3168/jds.20...
24.
Heppelmann M.,M., Weinert S.E., Ulbrich A., Brömmling M., Piechotta S., Merbach H.A., Schoon H.-A., Hoedemaker M., Bollwein H., 2016. The effect of puerperal uterine disease on histopathologic findings and mRNA expression of proinflammatory cytokines of the endometrium in dairy cows. Theriogenology 85, 1348–1356, https://doi.org/10.1016/j.ther...
25.
Kasimanickam R., Cornwell J.M., Nebel R.L., 2006. Effect of presence of clinical and subclinical endometritis at the initiation of PresynchOvsynch program on the first service pregnancy in dairy cows. Anim. Reprod. Sci. 95, 214-223, https://doi.org/10.1016/j.anir...
26.
Kasimanickam R., Duffield T.F., Foster R.A., Gartley C.J., Leslie K.E., Walton J.S., Johnson W.H., 2004. Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows. Theriogenology 62, 9-23, https://doi.org/10.1016/j.ther...
27.
Keskin A., Yilmazbas-Mecitoglu G., Gümen A., Karakaya E., Darici R., Okut H., 2010. Effect of hCG vs. GnRH at the beginning of the Ovsynch on first ovulation and conception rates in cyclic lactating dairy cows. Theriogenology. 74, 602–607, https://doi.org/10.1016/j.ther...
28.
Knudsen L.R., Karstrup C.C., Pedersen H.G., Agerholm J.S., Jensen T.K., Klitgaard K., 2015. Revisiting bovine pyometra - new insights into the disease using a culture-independent deep sequencing approach. Vet. Microbiol. 175, 319–324, https://doi.org/10.1016/j.vetm...
29.
McLaughlin C.L., Stanisiewski E., Lucas M.J., Cornell C.P., Watkins J., Bryson L., Tena J.K.S., Hallberg J., Chenault J.R., 2012. Evaluation of two doses of ceftiofur crystalline free acid sterile suspension for treatment of metritis in lactating dairy cows. J.Dairy Sci. 95, 4363–4371, https://doi.org/10.3168/jds.20...
30.
Nowicki A., Barański W., Tobolski D., Zduńczyk S., Janowski T., 2018. Second prostaglandin F2α treatment during Ovsynch protocol does not improve fertility outcomes in dairy cows. Pol. J. Vet. Sci. 22, 157–161, http://dx.doi.org/10.24425/pjv...
31.
Pascottini O.B., LeBlanc S.J., 2020. Metabolic markers for purulent vaginal discharge and subclinical endometritis in dairy cows. Theriogenology 155, 43–48, https://doi.org/10.1016/j.ther...
32.
Pursley J.R., Mee M.O., Wiltbank M.C., 1995. Synchronization of ovulation in dairy cows using PGF2a and GnRH. Theriogenology 44, 915–923, https://doi.org/10.1016/0093-6...
33.
Ribeiro E.S., Gomes G., Greco L.F., Cerri R.L.A., Vieira-Neto A., Monteiro P.L.J., Lima F.S., Bisinotto R.S., Thatcher W.W., Santos J.E.P., 2016. Carryover effect of postpartum inflammatory diseases on developmental biology and fertility in lactating dairy cows. J. Dairy Sci. 99, 2201–2220, https://doi.org/10.3168/jds.20...
34.
Sartori R., Haughian J.M., Shaver R.D., Rosa G.J., Wiltbank M.C., 2004. Comparison of ovarian function and circulating steroids in estrous cycles of Holstein heifers and lactating cows. J. Dairy Sci. 87, 905–920, https://doi.org/10.3168/jds.S0...
35.
Sheldon I.M., Cronin J., Goetze L., Donofrio G., Schuberth H.J., 2009a. Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle. Biol. Reprod. 81, 1025–1032, https://doi.org/10.1095/biolre...
36.
Sheldon I.M., Price S.B., Cronin J., Gilbert R.O., Gadsby J.E., 2009b. Mechanisms of infertility associated with clinical and subclinical endometritis in high producing dairy cattle. Reprod. Domest. Anim. 44, 1–9, https://doi.org/10.1111/j.1439...
37.
Spencer T.E., 2013. Early pregnancy: concepts, challenges, and potential solutions. Anim. Front. 3, 48–55, https://doi.org/10.2527/af.201...
38.
Strüve K., Herzog K., Magata F., Piechotta M., Shirasuna K., Miyamoto A., Bollwein H., 2013. The effect of metritis on luteal function in dairy cows. BMC Vet. Res. 9, 244, https://doi.org/10.1186/1746-6...
39.
Toni F., Vincenti L., Ricci A., Schukken Y.H., 2015. Postpartum uterine diseases and their impacts on conception and days open in dairy herds in Italy. Theriogenology 84, 1206–1214, https://doi.org/10.1016/j.ther...
40.
Vieira-Neto A., Gilbert R.O., Butler W.R., Santos J.E.P., Ribeiro E.S., Vercouteren M.M., Bruno R.G., Bittar J.H., Galvão K.N., 2014. Individual and combined effects of anovulation and cytological endometritis on the reproductive performance of dairy cows. J. Dairy Sci. 97, 5415–5425, https://doi.org/10.3168/jds.20...
41.
Williams E.J., 2013. Drivers of post-partum uterine disease in dairy cattle. Reprod. Domest. Anim. 48, 53–58, https://doi.org/10.1111/rda.12...
42.
Wiltbank M.C., Souza A.H., Carvalho P.D., Bender R.W., Nascimento A.B., 2011. Improving fertility to timed artificial insemination by manipulation of circulating progesterone concentrations in lactating dairy cattle. Reprod. Fertil. Dev. 24, 238–243, https://doi.org/10.1071/RD1191...
43.
Wittrock J.M., Proudfoot K.L., Weary D.M., von Keyserlingk M.A., 2011. Short communication: metritis affects milk production and cull rate of Holstein multiparous and primiparous dairy cows differently. J. Dairy Sci. 94, 2408–2412, https://doi.org/10.3168/jds.20...
| ISSN: | 1230-1388 |
Assigning DOI numbers, introducing articles from supplements and recent publications to POL-index database, maintaining anti-plagiarism detection, electronic system to proceed manuscripts and webpage of the Journal with interactive pdf files, and language correction of manuscripts published in Journal of Animal and Feed Sciences are financed in the years 2018–2019 by the Ministry of Science and Higher Education from the funds for science popularization activities, Agreement No. 631/P-DUN/2018.
© 2006-2024 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
