SHORT COMMUNICATION
Detection and in vitro regulation of NHE1 and
NHE3 mRNA expression in rumen epithelia of
cows
1
Kay Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University,
Nanjing 210095, P.R. China
2
Institute of Veterinary Physiology, Free University Berlin,
Oertzenweg 19b, 14163 Berlin, Germany
Publication date: 2007-09-17
Corresponding author
Z. Shen
Kay Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing 210095, P.R. China
Kay Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing 210095, P.R. China
J. Anim. Feed Sci. 2007;16(Suppl. 2):357-361
KEYWORDS
ABSTRACT
NHE (Na+/H+ exchanger) mediates electroneutral Na transport across rumen epithelium by
ejection of intracellular H+ in exchange of external Na+ with a ratio of 1:1. It contributes to the
absorption of Na+ and maintenance of intracellular pH homeostasis of rumen epithelium. This study
explored the existing of NHEs in rumen epithelia of cows and the responses of NHE1 and NHE3
mRNA to IGF-1. The results, obtained by semi-quantitative reverse transcription-polymerase chain
reaction (RT-PCR), illustrated that NHE1 and NHE3 mRNA is present in rumen epithelia of cows.
The NHE1 and NHE3 mRNA expression were promoted by IGF-1 treatment (25 ng/ml) in vitro. The
abundance of NHE1 and NHE3 mRNA were significantly higher in IGF-1 treated groups (P<0.05).
Taking together, the current study revealed that the mRNA of NHE1 and NHE3 in rumen epithelia
of cows could be positively regulated by IGF-1.
| ISSN: | 1230-1388 |
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