Effect of spermine on liver and spleen antioxidant status in weaned rats
G. Liu 1  
,   W. Cao 1,   G. Jia 1,   H. Zhao 1,   X. Chen 1,   J. Wang 2,   C. Wu 1
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Institute of Animal Nutrition, Sichuan Agricultural University and Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, Sichuan, China
Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
G. Liu   

Institute of Animal Nutrition, Sichuan Agricultural University and Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Chengdu 611130, Sichuan, China
Publication date: 2016-12-13
J. Anim. Feed Sci. 2016;25(4):335–342
Whereas strong antioxidant properties of spermine have been reported mostly in in vitro studies, there is lack of the in vivo studies on spermine influence conducted on mammals. The main objective of this study was to investigate the effects of different doses of spermine and the period of its supplementation on the liver and spleen antioxidant capacity in weaned rats. Male Sprague-Dawley rats at the age of 19 days received intragastrically spermine at the dose of 0.2 or 0.4 μmol · g-1 body weight for 3 or 7 days, respectively. Control rats received saline in analogical way. It was found that liver anti-superoxide anion (ASA) capacity, catalase (CAT) activity, glutathione (GSH) content and total antioxidant capacity (T-AOC) were increased in group supplemented with higher dose of spermine after 3 days, and anti-hydroxy radical (AHR) capacity was increased when treatment lasted for 7 days. In the spleen the higher spermine dose supplementation increased ASA capacity and total superoxide dismutase (T-SOD) activity (after 3 and 7 days), AHR capacity (after 7 days) and T-AOC (after 3 days) in comparison to the corresponding control groups (P < 0.05). Only in the spleen the lower spermine dose reduced lipid peroxidation level and increased CAT activity and GSH content regardless treatment duration (P < 0.05). The obtained results suggest that spermine supplementation can improve the antioxidant properties of the liver and spleen of weaned rats in a dose-, time- and tissue-dependent manner.
Aebi H., 1984. Catalase in vitro. Methods Enzymol. 105, 121–126, https://doi.org/10.1016/S0076-...
Benzie I.F.F., Strain J.J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal. Biochem. 239, 70–76, https://doi.org/10.1006/abio.1...
Bradford M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254, https://doi.org/10.1016/0003-2...
Campbell J.M., Crenshaw J.D., Polo J., 2013. The biological stress of early weaned piglets. J. Anim. Sci. Biotechnol. 4, 19, https://doi.org/10.1186/2049-1...
Cao W., Liu G., Fang T., Wu X., Jia G., Zhao H., Chen X., Wu C., Wang J., Cai J., 2015. Effects of spermine on the morphology, digestive enzyme activities, and antioxidant status of jejunum in suckling rats. RSC Adv. 5, 76607–76614, https://doi.org/10.1039/C5RA15...
Cipolla B.G., Havouis R., Moulinoux J.-P., 2010. Polyamine reduced diet (PRD) nutrition therapy in hormone refractory prostate cancer patients. Biomed. Pharmacother. 64, 363–368, https://doi.org/10.1016/j.biop...
Das K.C., Misra H.P., 2004. Hydroxyl radical scavenging and singlet oxygen quenching properties of polyamines. Mol. Cell. Biochem. 262, 127–133, https://doi.org/10.1023/B:MCBI...
David M., Munaswamy V., Halappa R., Marigoudar S.R., 2008. Impact of sodium cyanide on catalase activity in the freshwater exotic carp, Cyprinus carpio (Linnaeus). Pestic. Biochem. Phys. 92, 15–18, https://doi.org/10.1016/j.pest...
Deloyer P., Peulen O., Dandrifosse G., 2005. Intestinal effects of longlasting spermine ingestion by suckling rats. Exp. Physiol. 90, 901–908, https://doi.org/10.1113/expphy...
Fang T., Liu G., Cao W., Wu X., Jia G., Zhao H., Chen X., Wu C., Wang J., 2016. Spermine: new insights into the intestinal development and serum antioxidant status of suckling piglets. RSC Adv. 6, 31323–31335, https://doi.org/10.1039/C6RA05...
Ha H.C., Sirisoma N.S., Kuppusamy P., Zweier J.L., Woster P.M., Casero R.A. Jr., 1998. The natural polyamine spermine functions directly as a free radical scavenger. Proc. Natl. Acad. Sci. U.S.A. 95, 11140–11145, https://doi.org/10.1073/pnas.9...
Jiang J., Zheng T., Zhou X.-Q., Liu Y., Feng L., 2009. Influence of glutamine and vitamin E on growth and antioxidant capacity of fish enterocytes. Aquacult. Nutr. 15, 409–414, https://doi.org/10.1111/j.1365...
Kafy A.M.L., Haigh C.G., Lewis D.A., 1986. In vitro interactions between endogenous polyamine and superoxide anion. Agents Actions 18, 555–559, https://doi.org/10.1007/BF0196...
Liu G., Fang T., Yan T., Jia G., Zhao H., Huang Z., Chen X., Wang J., Xue B., 2014. Metabolomic strategy for the detection of metabolic effects of spermine supplementation in weaned rats. J. Agric. Food Chem. 62, 9035–9042, https://doi.org/10.1021/jf5008...
Liu G., Yan T., Fang T., Jia G., Chen X., Zhao H., Wang J., Wu C., 2015. Nutrimetabolomic analysis provides new insights into spermine-induced ileum-system alterations for suckling rats. RSC Adv. 5, 48769–48778, https://doi.org/10.1039/C5RA01...
Livingstone D.R., Martinez P.G., Michel X., Narbonne J.F., O’Hara S., Ribera D., Winston G.W., 1990. Oxyradical production as a pollution-mediated mechanism of toxicity in the common mussel, Mytilus edulis L., and other molluscs. Funct. Ecol. 4, 415–424, https://doi.org/10.2307/238960...
Mallikarjuna K., Nishanth K., Sathyavelu Reddy K., 2007. Hepatic glutathione mediated antioxidant system in ethanol treated rats: decline with age. Pathophysiology 14, 17–21, https://doi.org/10.1016/j.path...
Moeser A.J., Ryan K.A., Nighot P.K., Blikslager A.T., 2007. Gastrointestinal dysfunction induced by early weaning is attenuated by delayed weaning and mast cell blockade in pigs. Am. J. Physiol. Gastrointest. Liver Physiol. 293, G413-G421, https://doi.org/10.1152/ajpgi....
Pegg A.E., 2014. The function of spermine. IUBMB Life 66, 8–18, https://doi.org/10.1002/iub.12...
Peulen O., Dandrifosse G., 2000. Cyclosporine A inhibits partially spermine-induced differentiation but not cell loss of suckling rat small intestine. Digest. Dis. Sci. 45, 750–754, https://doi.org/10.1023/A:1005...
Pirinccioglu A.G., Gökalp D., Pirinccioglu M., Kizil G., Kizil M., 2010. Malondialdehyde (MDA) and protein carbonyl (PCO) levels as biomarkers of oxidative stress in subjects with familial hypercholesterolemia. Clin. Biochem. 43, 1220–1224, https://doi.org/10.1016/j.clin...
Ren W., Yin Y., Liu G., Yu X., Li Y., Yang G., Li T., Wu G., 2012. Effect of dietary arginine supplementation on reproductive performance of mice with porcine circovirus type 2 infection. Amino Acids 42, 2089–2094, https://doi.org/10.1007/s00726...
Romain N., Dandrifosse G., Jeusette F., Forget P., 1992. Polyamine concentration in rat milk and food, human milk, and infant formulas. Pediatr. Res. 32, 58–63, https://doi.org/10.1203/000064...
Sava I.G., Battaglia V., Rossi C.A., Salvi S., Toninello A., 2006. Free radical scavenging action of the natural polyamine spermine in rat liver mitochondria. Free Radic. Biol. Med. 41, 1272–1281, https://doi.org/10.1016/j.free...
Valko M., Leibfritz D., Moncol J., Cronin M.T.D., Mazur M., Telser J., 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39, 44–84, https://doi.org/10.1016/j.bioc...
Vardi N., Parlakpinar H., Ozturk F., Ates B., Gul M., Cetin A., Erdogan A., Otlu A., 2008. Potent protective effect of apricot and β-carotene on methotrexate-induced intestinal oxidative damage in rats. Food Chem. Toxicol. 46, 3015–3022, https://doi.org/10.1016/j.fct....
Zhang X.D., Zhu Y.F., Cai L.S.,Wu T.X., 2008. Effects of fasting on the meat quality and antioxidant defenses of market-size farmed large yellow croaker (Pseudosciaena crocea). Aquaculture 280, 136–139, https://doi.org/10.1016/j.aqua...
Zhu L., Cai X., Guo Q., Chen X., Zhu S., Xu J., 2013. Effect of N-acetyl cysteine on enterocyte apoptosis and intracellular signalling pathways’ response to oxidative stress in weaned piglets. Br. J. Nutr. 110, 1938–1947, https://doi.org/10.1017/S00071...
Zhu L.H., Zhao K.L., Chen X.L., Xu J.X., 2012. Impact of weaning and an antioxidant blend on intestinal barrier function and antioxidant status in pigs. J. Anim. Sci. 90, 2581–2589, https://doi.org/10.2527/jas.20...
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