Two experiments were performed with pigs fed peas. Pigs were fitted with a T-shaped cannula at the end of the ileum. The animals were fed three times daily and two series of ileal digesta collections were carried out during the day and night. The ileal samples were pooled in three intervals: morning, evening and night. In Experiment 1 the samples were collected and frozen every 2-3 h during the morning and evening intervals, and every 9 h in the night interval, whereas in Experiment 2, the samples were collected on a continuous basis and frozen immediately. In Experiment 1, lactic acid and short chain fatty acid (SCFA) concentrations, and the digestibilities of dry matter and non-starch polysaccharides increased from morning to night. The molar ratio of SCFA was also affected by the interval. In Experiment 2, no differences were observed in the mentioned parameters between intervals. Results of the experiments indicate that there are no differences between day and night in digestibility of nutrients when pigs are fed pea-based diet. The collection of ileal digesta during 8 h for digestibility measurements is representative for 24 h period when animals were fed at 8 h intervals. Ileal samples of digesta should be collected frequently and frozen when pH, SCFA are to be measured.