ORIGINAL PAPER
 
KEYWORDS
TOPICS
ABSTRACT
The objective of this study was to evaluate the effects of a multi-enzyme complex containing xylanase, amylase, and protease (XAP) on growth performance, apparent digestibility, blood profile, intestinal villus height, and faecal gas emission in weaning pigs. One hundred and twenty-eight piglets [(Yorkshire × Landrace) × Duroc] were used in a two-phase feeding trial: phase I (days 1 to 14) and phase II (days 15 to 42). Piglets were randomly allotted to two treatments, one 100 mg/kg XAP (xylanases, 2000 U/kg diet; amylases, 200 U/kg diet; proteases, 2000 U/kg diet) and one without, with 16 replicates (two barrows and two gilts) per pen. In phase II, the XAP group showed increased average daily gain (ADG) (P < 0.05) compared to the control group. At the end of the feeding trial, piglets supplemented with XAP showed enhanced ADG and gain-to-feed ratio (P < 0.05). Moreover, pigs fed the XAP diet had a higher (P < 0.05) duodenal and jejunal villus height compared to the control group. There were no significant differences in other parameters. The inclusion of 100 mg/kg XAP in maize-soybean meal-based diets improved growth performance and intestinal villus height in piglets, suggesting its potential benefits for the growth and gut health of these animals.
FUNDING
This research was financially supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRFRS- 2023-00275307).
CONFLICT OF INTEREST
The Authors declare that there is no conflict of interest.
 
REFERENCES (30)
1.
Amerah A.M., Romero L.F., Awati A., Ravindran V., 2017. Effect of exogenous xylanase, amylase, and protease as single or combined activities on nutrient digestibility and growth performance of broilers fed corn/soy diets. Poult. Sci. 96, 807–816, https://doi.org/10.3382/ps/pew....
 
2.
AOAC, 2000. Official methods of analysis of AOAC International. 17th Ed., Washington (DC).
 
3.
Cai L., Indrakumar S., Kiarie E., Kim I.H., 2015. Effects of a multi-strain bacillus species-based direct-fed microbial on growth performance, nutrient digestibility, blood profile, and gut health in nursery pigs fed corn-soybean meal-based diets. J. Anim. Sci. 93, 4336–4342, https://doi.org/10.2527/jas.20....
 
4.
Diebold G., Mosenthin R., Piepho H.P., Sauer W.C., 2004. Effect of supplementation of xylanase and phospholipase to a wheat-based diet for weaning pigs on nutrient digestibility and concentrations of microbial metabolites in ileal digesta and feces. J. Anim. Sci. 82, 2647–2656, https://doi.org/10.2527/2004.8....
 
5.
Diebold G., Mosenthin R., Sauer W.C., Dugan M.E.R., Lien K.A., 2005. Supplementation of xylanase and phospholipase to wheat-based diets for weaner pigs. J. Anim. Physiol. Anim. Nutr. 89, 316–325, https://doi.org/10.1111/j.1439....
 
6.
Fan C.L., Han X.Y., Xu Z.R., Wang L.J., Shi L.R., 2009. Effects of beta-glucanase and xylanase supplementation on gastrointestinal digestive enzyme activities of weaned piglets fed a barley-based diet. J. Anim. Physiol. Anim. Nutr. 93, 271–276, https://doi.org/10.1111/j.1439....
 
7.
Huisman M.M.H., Schols H.A., Voragen A.G.J., 1998. Cell wall polysaccharides from soybean (Glycine max) meal. Isolation and characterization. Carbohydr. Polym. 37, 87–95, https://doi.org/10.1016/S0144-....
 
8.
Kiarie E., Owusu-Asiedu A., Peron A., Simmins P.H., Nyachoti C.M., 2012. Efficacy of xylanase and β-glucanase blend in mixed grains and grain co-products-based diets for fattening pigs. Livest. Sci. 148, 129–133, https://doi.org/10.1016/j.livs....
 
9.
Kiarie E., Petracek R., 2015. Growth performance of nursery pigs fed pelleted wheat based diets containing graded levels of supplemental xylanase. Anim. Prod. Sci. 55, 1548, https://doi.org/10.1071/ANv55n....
 
10.
Kiarie E., Romero L.F., Nyachoti C.M., 2013. The role of added feed enzymes in promoting gut health in swine and poultry. Nutr. Res. Rev. 26, 71–88, https://doi.org/10.1017/S09544....
 
11.
Kohn R.A., Dinneen M.M., Russek-Cohen E., 2005. Using blood urea nitrogen to predict nitrogen excretion and efficiency of nitrogen utilization in cattle, sheep, goats, horses, pigs, and rats. J. Anim. Sci. 83, 879–889, https://doi.org/10.2527/2005.8....
 
12.
Knudsen K.E.B., 1997. Carbohydrate and lignin contents of plant materials used in animal feeding. Anim. Feed Sci. Technol. 67, 319–338, https://doi.org/10.1016/S0377-....
 
13.
Liu W.C., Kim I.H., 2016. Effects of dietary xylanase supplementation on performance and functional digestive parameters in broilers fed wheat-based diets. Poult. Sci. 96, 566–573, https://doi.org/10.3382/ps/pew....
 
14.
Munezero Olivier., Kim I.H., 2022. Effects of protease enzyme supplementation in weanling pigs’ diet with different crude protein levels on growth performance and nutrient digestibility. J. Anim. Sci. Technol. 64, 854–862, https://doi.org/10.5187/jast.2....
 
15.
NRC, 2012. Nutrient requirements of swine. 11th Ed., National Academy Press, Washington (DC).
 
16.
Olukosi O.A., Sands J.S., Adeola O., 2007. Supplementation of carbohydrases or phytase individually or in combination to diets for weaning and growing-finishing pigs. J. Anim. Sci. 85, 1702–1711, https://doi.org/10.2527/jas.20....
 
17.
O'Shea C.J., Mc Alpine P.O., Solan P., Curran T., Varley P.F., 2014. Walsh A.M., Doherty J.V., The effect of protease and xylanase enzymes on growth performance, nutrient digestibility, and manure odour in grower–finisher pigs. Anim. Feed Sci. Tech. 189, 88–89, https://doi.org/10.1016/j.anif....
 
18.
Owusu-Asiedu A., Simmins P.H., Brufau J., Lizardo R., Peron A., 2010. Effect of xylanase and β-glucanase on growth performance and nutrient digestibility in piglets fed wheat-barley-based diets. Livest. Sci. 1, 76–78, https://doi.org/10.1016/j.livs....
 
19.
Payling L., Kim I.H., Walsh M.C., Kiarie E., 2017. Effects of a multi-strain Bacillus spp. direct-fed microbial and a protease enzyme on growth performance, nutrient digestibility, blood characteristics, faecal microbiota, and noxious gas emissions of grower pigs fed corn-soybean-meal-based diets-A meta-analysis. J. Anim. Sci. 95, 4018–4029, https://doi.org/10.2527/jas.20....
 
20.
Pluske J.R., Hampson D.J., Williams I.H., 1997. Factors influencing the structure and function of the small intestine in the weaned pig: a review. Livest. Prod. Sci. 51, 215–236, https://doi.org/10.1016/S0301-....
 
21.
Puchal F., Hays V.W., Speer V.C., Jones J.D., Catron D.V., 1962. The free blood plasma amino acids of swine as related to the source of dietary proteins. J. Nutr. 76, 11–16, https://doi.org/10.1093/jn/76.....
 
22.
Sangwoo P., Wenting L., Benoit S.P., Qiong W., Awori W.T., 2020. Growth performance, nutrient digestibility, and faecal microbial composition of weaned pigs fed multi-enzyme supplemented diets. J. Anim. Sci. 98, https://doi.org/10.1093/jas/sk....
 
23.
Scheller H.V., Ulvskov P., 2010. Hemicelluloses. Annu. Rev. Plant Biol. 61, 263–289, https://doi.org/10.1146/annure...
 
24.
Sterk A., Verdonk J.M.A.J., Mul A.J., Soenen B., Bezencon M.L., Frehner M., Losa R., 2017. Effect of xylanase supplementation to a cereal-based diet on the apparent faecal digestibility in weaning piglets. Livest. Sci. 108, 269–271, https://doi.org/10.1016/j.livs....
 
25.
Tactacan G.B., Cho S.Y., Cho J.H., Kim I.H., 2016. Performance responses, nutrient digestibility, blood characteristics, and measures of gastrointestinal health in weanling pigs fed protease enzyme. Asian-Australas. J. Anim. Sci. 29, 998–1003, https://doi.org/10.5713/ajas.1....
 
26.
Tapingkae W., Yachai M., Visessanguan W., Pongtanya P., Pongpiachan P., 2008. Influence of crude xylanase from Aspergillus niger FAS128 on the in vitro digestibility and production performance of piglets. Anim. Feed Sci. Technol. 140, 141–154, https://doi.org/10.1016/j.anif....
 
27.
Willamil J., Badiola I., Devillard E., Geraert P.A., Torrallardona D., 2012. Wheat-barley-rye-or corn-fed growing pigs respond differently to dietary supplementation with a carbohydrase complex. J. Anim. Sci. 90, 824–832, https://doi.org/10.2527/jas.20....
 
28.
Yao K., Guan S., Li T., Huang R., Wu G., Ruan Z., Yin Y., 2011. Dietary l-arginine supplementation enhances intestinal development and expression of vascular endothelial growth factor in weaning piglets. Bri. J. Nutr. 105, 703–709, https://doi.org/10.1017/S00071....
 
29.
Yin Y., Baidoo S.K., Schulze H., Simmins P.H., 2001. Effects of supplementing diets containing hulless barley varieties having different levels of non-starch polysaccharides with -glucanase and xylanase on the physiological status of the gastrointestinal tract and nutrient digestibility of weaned pigs. Livest. Prod. Sci. 71, 97–107, https://doi.org/10.1016/S0301-....
 
30.
Zhang G.G., Yang Z.B., Wang Y., Yang W.R., Zhou H.J., 2014. Effects of dietary supplementation of multi-enzyme on growth performance, nutrient digestibility, small intestinal digestive enzyme activities, and large intestinal selected microbiota in weaning pigs. J. Anim. Sci. 92, 2063–2069, https://doi.org/10.2527/jas.20....
 
ISSN:1230-1388
Journals System - logo
Scroll to top