ORIGINAL PAPER
Developmental competence of bovine IVM/IVF oocytes under different co-culture conditions
L. Kątska 1,   B. Ryńska 1,   Z. Smorąg 1
 
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Research Institute of Animal Production, Department of Animal Reproduction, 32-083 Balice, Poland
Publication date: 1998-06-24
 
J. Anim. Feed Sci. 1998;7(3):353–362
 
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ABSTRACT
The experiment was carried out to compare the influence of different culture systems on the developmental potential of bovine embryos derived from IVM/IVF oocytes. The investigations included comparison of the developmental competence of IVM/IVF zygotes derived from oocytes cultured in large (>30 oocytes/well) and small (1-5 oocytes/drop of medium) groups and co-cultured with: Buffalo rat liver cells (BRL), African Green monkey kidney (VERO) and bovine oviduct epithelial cells (BOEC). The highest number of embryos derived from IVM/IVF oocytes maturing in large groups developed to blastocysts in co-culture with VERO (45.9%), followed by BRL (30.9%) and BOE (36.1%) cells. The hatching ability of these blastocysts did not differ significantly for BRL and VERO cocultures (respectively 74.5 and 58.0%) and was significantly lower for those co-cultured with BOEC (34.7%). In contrast to the oocytes maturing in large groups, the developmental competence of oocytes of the small group cultures was independent of the feeder layer used for co-culture and 19.4 to 27.1% of these oocytes reached the blastocyst stage. No significant differences were noticed in either the blastocyst rates or in the hatching ability of these blastocysts developing in co-culture with BRL, VERO and BOE cells. The mean number of cells in the blastocyst of the large group culture developing in co-culture with BRL cells on day 8 was 112.0±27.7 while the blastocysts developing in co-cultures with VERO and BOE cells contained 76.8±20.0 and 94.0±23.6 cells, respectively.
 
CITATIONS (3):
1.
The effect of Ureaplasma diversum activated mononuclear leukocytes on the development and interferon-τ production by bovine IVF-derived embryos
Anna Chełmońska-Soyta, Lucyna Kątska, Maciej Kurpisz, Tadeusz Stefaniak, Michał Zimecki
Journal of Reproductive Immunology
 
2.
Timing of nuclear maturation of nonstored and stored domestic cat oocytes
Lucyna Kątska-Książkiewicz, Bożenna Ryńska, Gabriela Kania, Zdzisław Smorąg, Barbara Gajda, Marek Pieńkowski
Theriogenology
 
3.
Effect of donor stimulation, frozen semen and heparin treatment on the efficiency of in vitro embryo production in goats
Lucyna Ka̧tska-Ksia̧żkiewicz, Bożenna Ryńska, Barbara Gajda, Zdzisław Smora̧g
Theriogenology
 
ISSN:1230-1388