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Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research

E. Pers-Kamczyc 1  ,  
P. Zmora 1,  
A. Cieślak 1,  
M. Szumacher-Strabel 1
 
1
Poznan University of Life Sciences, Department of Animal Nutrition and Feed Management, RUMEN PULS, Wołyńska 33, 60-637 Poznań, Poland
J. Anim. Feed Sci. 2011;20(3):315–337
Publication date: 2011-08-04
DOI: https://doi.org/10.22358/jafs/66189/2016
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Citations (16)
 
KEYWORDS
rumen, microorganisms, molecular techniques, real time PCR, PCR-DGGE, FISH
ABSTRACT
Despite many years of research elucidating processes occurring in the rumen, still only around 10% of the rumen microbe is known. Molecular biology techniques seem to be a good complement of an information obtained by using the classical microbiology and therefore can enhance our knowledge about processes occurring in the rumen. The aim of this paper is to describe and to summarize a widely used techniques of the molecular biology during analysis of microbes in rumen. The main attention have been focused on the three of them which can be employed for quantitative and qualitative analysis of rumen microorganisms: PCR-DGGE, Real-time PCR and Fluorescence in situ hybridization.
CORRESPONDING AUTHOR
E. Pers-Kamczyc   
Poznan University of Life Sciences, Department of Animal Nutrition and Feed Management, RUMEN PULS, Wołyńska 33, 60-637 Poznań, Poland
 
CITATIONS (16):
1. Feeding, Evaluating, and Controlling Rumen Function
Ian J. Lean, Helen M. Golder, Mary Beth Hall
Veterinary Clinics of North America: Food Animal Practice
2. An overview of the role of rumen methanogens in methane emission and its reduction strategies
Karri Satyanagalakshmi, Goud Talla Sridhar, S. K. Sirohi
African Journal of Biotechnology
3. Effects of partial mixed rations and supplement amounts on milk production and composition, ruminal fermentation, bacterial communities, and ruminal acidosis
H.M. Golder, S.E. Denman, C. McSweeney, W.J. Wales, M.J. Auldist, M.M. Wright, L.C. Marett, J.S. Greenwood, M.C. Hannah, P. Celi, E. Bramley, I.J. Lean
Journal of Dairy Science
4. Oscillibacter ruminantium sp. nov., isolated from the rumen of Korean native cattle
G.-H. Lee, M.-S. Rhee, D.-H. Chang, J. Lee, S. Kim, M. H. Yoon, B.-C. Kim
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
5. Plant components with specific activities against rumen methanogens
A. Cieslak, M. Szumacher-Strabel, A. Stochmal, W. Oleszek
animal
6. Effect ofMentha piperitaL. onin vitrorumen methanogenesis and fermentation
P. Zmora, A. Cieslak, E. Pers-Kamczyc, A. Nowak, J. Szczechowiak, M. Szumacher-Strabel
Acta Agriculturae Scandinavica, Section A - Animal Science
7. Metabolic Disorders in the Transition Period Indicate that the Dairy Cows’ Ability to Adapt is Overstressed
Albert Sundrum
Animals
8. Rumen antimethanogenic effect of Saponaria officinalis L. phytochemicals in vitro
A. CIESLAK, P. ZMORA, A. STOCHMAL, L. PECIO, W. OLESZEK, E. PERS-KAMCZYC, J. SZCZECHOWIAK, A. NOWAK, M. SZUMACHER-STRABEL
The Journal of Agricultural Science
9. Effects of tannins source (Vaccinium vitis idaea L.) on rumen microbial fermentation in vivo
Adam Cieslak, Pawel Zmora, Emilia Pers-Kamczyc, Malgorzata Szumacher-Strabel
Animal Feed Science and Technology
10. The influence of plant polyphenols from oil palm (Elaeis guineensis Jacq.) leaf extract on fermentation characteristics, biohydrogenation of C18 PUFA, and microbial populations in rumen of goats: in vitro study
A. Aiman-Zakaria, G. Yong-Meng, M. Ali-Rajion, S. Jafari, M. Faseleh-Jahromi, P. Shokriyazdan, M. Ebrahimi
Acta Agriculturae Scandinavica, Section A — Animal Science
11. Modifications and optimization of manual methods for polymerase chain reaction and 16S rRNA gene sequencing quality community DNA extraction from goat rumen digesta
Durgadevi Aphale, Aarohi Kulkarni
Veterinary World
12. Dietary Phytochemicals and Microbes
Malgorzata Szumacher-Strabel, Adam Cieślak
13. The effect of fumonisins producing Fusarium verticillioides on the microbiota in pig caecum
Huu Dang, Attila Zsolnai, Melinda Kovács, Brigitta Bóta, Gábor Mihucz, Roland Pósa, Kinga Marosi, Mariam Kachlek, Judit Szabó-Fodor
Acta Veterinaria Brno
14. Effects of Two Sources of Tannins (QuercusL. andVaccinium Vitis IdaeaL.) on Rumen Microbial Fermentation: anin VitroStudy
Adam Cieslak, Pawel Zmora, Emilia Pers-Kamczyc, Anna Stochmal, Anna Sadowinska, Abdelfattah Salem, Dorota Kowalczyk, Piotr Zbonik, Malgorzata Szumacher-Strabel
Italian Journal of Animal Science
15. Administration of Streptococcus bovis isolated from sheep rumen digesta on rumen function and physiology as evaluated in a rumen simulation technique system
Durgadevi Aphale, Aamod Natu, Sharad Laldas, Aarohi Kulkarni
Veterinary World
16. Rumen and Hindgut Bacteria Are Potential Indicators for Mastitis of Mid-Lactating Holstein Dairy Cows
Yifan Zhong, Ming-Yuan Xue, Hui-Zeng Sun, Teresa Valencak, Le Guan, Jianxin Liu
Microorganisms
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