Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research

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Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research

E. Pers-Kamczyc ¹

,

P. Zmora ¹

,

A. Cieślak ¹

,

M. Szumacher-Strabel ¹

1

Poznan University of Life Sciences, Department of Animal Nutrition and Feed Management, RUMEN PULS, Wołyńska 33, 60-637 Poznań, Poland

Publication date: 2011-08-04

Corresponding author

E. Pers-Kamczyc

Poznan University of Life Sciences, Department of Animal Nutrition and Feed Management, RUMEN PULS, Wołyńska 33, 60-637 Poznań, Poland

J. Anim. Feed Sci. 2011;20(3):315-337

DOI: https://doi.org/10.22358/jafs/66189/2016

KEYWORDS

molecular techniques

ABSTRACT

Despite many years of research elucidating processes occurring in the rumen, still only around 10% of the rumen microbe is known. Molecular biology techniques seem to be a good complement of an information obtained by using the classical microbiology and therefore can enhance our knowledge about processes occurring in the rumen. The aim of this paper is to describe and to summarize a widely used techniques of the molecular biology during analysis of microbes in rumen. The main attention have been focused on the three of them which can be employed for quantitative and qualitative analysis of rumen microorganisms: PCR-DGGE, Real-time PCR and Fluorescence in situ hybridization.

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20.

Potential of Paulownia leaves silage in lamb diet to improve ruminal fermentation and fatty acid profile – an in vitro study
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Annals of Animal Science

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