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2/2019 vol. 28
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Changes of β-carotene and retinol levels and BCO1 gene and protein expressions in yak tissues at different nutritional seasons

D.-X. Cao 1,  
S.-L. Wang 1, 2  ,  
R. WANG 1,  
S.-T. Chai 3,  
S.-J. Liu 2, 3,  
L.-Z. Hao 3
 
1
Qinghai University, Agriculture and Animal Husbandry College, 251 Ningda road, Chengbei district, Xining city, Qinghai province, 810016, China
2
Qinghai University, State Key Laboratory of Plateau Ecology and Agriculture, 251 Ningda road, Chengbei district, Xining city, Qinghai province, 810016, China
3
Qinghai University, Institute of Animal and Veterinary Science, 1 Weier road, Biotechnology park, Chengbei districtrict, Xining city, Qinghai province, 810016, China
J. Anim. Feed Sci. 2019;28(2):120–127
Publication date: 2019-06-19
DOI: https://doi.org/10.22358/jafs/109953/2019
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References (32)
 
KEYWORDS
yaks, beta-carotene, gene expression, real time PCR, retinol
TOPICS
meat quality, nutrition, vitamins, metabolic syndrome and obesity
ABSTRACT
In order to explore relationship between the yellow fat and β-carotene metabolism in yaks, contents of β-carotene and retinol in tissues, serum and digestive tract contents were analysed at periods of rich and deficient nutrition. Expressions of β-carotene 15, 15’-monooxygenase 1 (BCO1) gene and protein were also studied using real-time PCR and western blot. It was shown that contents of β-carotene in serum, liver, pancreas, duodenum, jejunum and contents of rumen and ileum were higher in the period of rich nutrition than in the period of deficient nutrition (P < 0.05). Content of retinol in liver was much higher in the period of rich nutrition than in the period of deficient nutrition (P < 0.05). Expression levels of BCO1 mRNA in kidney, jejunum, ileum, duodenum, muscle and rumen in the period of rich nutrition were significantly lower than in the period of deficient nutrition (P < 0.05), besides pancreas. Expressions of BCO1 protein in duodenum, jejunum, kidney, muscle, and rumen were lower in the period of rich nutrition than in the period of deficient nutrition (P < 0.05).
CORRESPONDING AUTHOR
S.-L. Wang   
Qinghai University, Agriculture and Animal Husbandry College, 251 Ningda road, Chengbei district, Xining city, Qinghai province, 810016, China
 
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