Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test
P. Klein 1  
,   J. Moravcová 2,   T. Kleinová 1,   Z. Volek 1,   V. Skřivanová 1
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Research Institute of Animal Production, Praha-Uhřineves, CZ-104 01, Czech Republic
Department of Chemistry of Natural Compounds, Institute of Chemical Technology, Praha, CZ-166 28, Czech Republic
P. Klein   

Research Institute of Animal Production, Praha-Uhřineves, CZ-104 01, Czech Republic
Publication date: 2007-02-02
J. Anim. Feed Sci. 2007;16(1):43–52
The aim of this study was to utilize the lactulose/mannitol test for assessment of intestinal permeability (IP) in preruminant calves. IP was increased experimentally by administration of Indomethacin. Both sugar markers were determined simultaneously as silyl-derivates in 5 h urinary production using the gas liquid chromatography technique. The index of IP was determined as the ratio between urinary recovery (%) of 10 g lactulose to 5 g D-mannitol. The IP index in control calves was 0.242±0.048. Doses of 20 and 60 mg Indomethacin did not significantly affect IP, but the IP index was significantly enhanced in calves receiving 120 mg of drug (0.541±0.091; P<0.01). Results of this study show that ratio of lactulose to D-mannitol in urine reflected the treatment by the highest dose of Indomethacin and that the test may be used for determination of IP in preruminant calves.
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