ORIGINAL PAPER
Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test
P. Klein 1  
,   J. Moravcová 2,   T. Kleinová 1,   Z. Volek 1,   V. Skřivanová 1
 
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1
Research Institute of Animal Production, Praha-Uhřineves, CZ-104 01, Czech Republic
2
Department of Chemistry of Natural Compounds, Institute of Chemical Technology, Praha, CZ-166 28, Czech Republic
CORRESPONDING AUTHOR
P. Klein   

Research Institute of Animal Production, Praha-Uhřineves, CZ-104 01, Czech Republic
Publication date: 2007-02-02
 
J. Anim. Feed Sci. 2007;16(1):43–52
 
KEYWORDS
ABSTRACT
The aim of this study was to utilize the lactulose/mannitol test for assessment of intestinal permeability (IP) in preruminant calves. IP was increased experimentally by administration of Indomethacin. Both sugar markers were determined simultaneously as silyl-derivates in 5 h urinary production using the gas liquid chromatography technique. The index of IP was determined as the ratio between urinary recovery (%) of 10 g lactulose to 5 g D-mannitol. The IP index in control calves was 0.242±0.048. Doses of 20 and 60 mg Indomethacin did not significantly affect IP, but the IP index was significantly enhanced in calves receiving 120 mg of drug (0.541±0.091; P<0.01). Results of this study show that ratio of lactulose to D-mannitol in urine reflected the treatment by the highest dose of Indomethacin and that the test may be used for determination of IP in preruminant calves.
 
CITATIONS (7):
1.
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2.
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Journal of Dairy Science
 
3.
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Hypertonic milk replacers increase gastrointestinal permeability in healthy dairy calves
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5.
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L. Amado, H. Berends, L.N. Leal, J. Wilms, H. Van Laar, W.J.J. Gerrits, J. Martín-Tereso
Journal of Dairy Science
 
6.
Technical note: fluorescein as an indicator of enteric mucosal barrier function in preruminant lambs
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Journal of Animal Science
 
7.
Use of aspirin to intentionally induce gastrointestinal tract barrier dysfunction in feedlot cattle
Nathan Briggs, Kristen Brennan, Bethany Funnell, Griffin Nicholls, Jon Schoonmaker
Journal of Animal Science
 
ISSN:1230-1388