ORIGINAL PAPER
Effect of variation in the proportion of solid- and liquid-associated rumen bacteria in duodenal contents on the estimation of duodenal bacterial nitrogen flow
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Laboratory for Animal Nutrition and Animal Product Quality, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
 
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Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB, UK
 
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Current address: Agriculture and Life Sciences Division, Lincoln University, Canterbury, New Zealand
 
 
Publication date: 2007-02-02
 
 
Corresponding author
V. Fievez   

Laboratory for Animal Nutrition and Animal Product Quality, Ghent University, Proefhoevestraat 10, 9090 Melle, Belgium
 
 
J. Anim. Feed Sci. 2007;16(1):37-42
 
KEYWORDS
ABSTRACT
The aim of this research was to examine to what extent variation in the relative proportions of solid- (SAB) and liquid-associated rumen bacteria (LAB) in duodenal bacteria have an impact on the estimation of duodenal flow of bacterial N. For this, four dairy cows were fed diets varying in forage: concentrate ratio (80:20, 65:35, 50:50 and 35:65). SAB and LAB were separated from rumen contents four h after the morning feeding. Adenine, cytosine and odd and branched-chain fatty acids were determined both in SAB and LAB and used to estimate bacterial N flow. Bacterial N flows were also calculated using a SAB:LAB ratio in duodenal bacteria, as estimated from the odd and branched-chain fatty acid pattern. Compared with calculations based on the estimated SAB:LAB ratio, estimations based on SAB or LAB only as a bacterial reference on average over- and underestimated bacterial N flow by 37 and 55 g N/d, respectively (P<0.05) when cytosine or adenine were used as bacterial marker. In contrast, due to the small differences in the OBCFA:N ratio between SAB and LAB, these differences were less than 15 g/d when OBCFA were used as bacterial marker. The results suggest that, depending on the marker used, changes in the proportions of SAB and LAB can have a substantial impact on estimated duodenal flow of bacterial N.
 
CITATIONS (4):
1.
Relationship between milk odd and branched-chain fatty acids and urinary purine derivatives in dairy cows supplemented with quebracho tannins—A study to test milk fatty acids as predictors of rumen microbial protein synthesis
J. Castro-Montoya, A. Henke, J. Molkentin, K. Knappstein, A. Susenbeth, U. Dickhoefer
Animal Feed Science and Technology
 
2.
Milk odd- and branched-chain fatty acids as biomarkers of rumen function—An update
V. Fievez, E. Colman, J.M. Castro-Montoya, I. Stefanov, B. Vlaeminck
Animal Feed Science and Technology
 
3.
The relationship between odd- and branched-chain fatty acids and microbial nucleic acid bases in rumen
Keyuan Liu, Xiaoyan Hao, Yang Li, Guobin Luo, Yonggen Zhang, Hangshu Xin
Asian-Australasian Journal of Animal Sciences
 
4.
The relationships of dairy ruminal odd- and branched- chain fatty acids to the duodenal bacterial nitrogen flow and volatile fatty acids
Keyuan Liu, Yang Li, Guobin Luo, Hangshu Xin, Yonggen Zhang, Guangyu Li
Livestock Science
 
ISSN:1230-1388
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