0.906
IF5
0.875
IF
Q3
JCR
1.0
CiteScore
0.374
SJR
Q2
SJR
20
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Validation of a method for simultaneous determination of tocopherols and tocotrienols in cereals using Normal Phase HPLC

R. Gąsior 1  ,  
M. Pieszka 2,  
 
1
National Research Institute of Animal Production, Central Laboratory, 32-084 Morawica, Poland
2
National Research Institute of Animal Production, Department of Animal Nutrition and Feed Science, 32-083 Balice, Poland
J. Anim. Feed Sci. 2009;18(1):173–192
Publish date: 2009-01-23
KEYWORDS
ABSTRACT
A method for the simultaneous determination of tocols (α-, β-,γ-, δ-tocopherols and α-, β-,γ-, δ-tocotrienols) in cereals using Normal Phase-HPLC (Merck; column LiChroCART 250-4, Lichrospher Si 60, 5 μm) with fluorescent detection (Ex290 nm/Em350 nm) was validated. A mixture of hexane, ethyl acetate and acetic acid (97.3:1.8:0.9, v/v/v) was used as the eluent (1.6 ml/min). The analyses were performed after spectrophotometric standardization of standard ethanol solutions. The residual coefficients of variation for the regression equation y= ax2 + bx + c were 0.001-3.8%, with r2>0.999. The limit of quantitation was 0.05 mg/kg, the upper limit of determination range from 40 to 60 mg/kg, repeatability 6.2-11.8%, and reproducibility 8.7-18.4%. Uncertainty of the method (P≤0.05), estimated for the analyses performed in replicate, ranged from 28.9 to 85.9% for the tocol concentration of 0.05-0.80 μg/ml and from 14.7 to 27.3% for the concentrations above 0.80 μg/ml. Recovery was 93.3-103.1%. The analytical method described is precise, fast and inexpensive.
CORRESPONDING AUTHOR
R. Gąsior   
National Research Institute of Animal Production, Central Laboratory, 32-084 Morawica, Poland
 
CITATIONS (7):
1. Evaluation of Fatty Acid Composition, Tocols Profile, and Oxidative Stability of Some Fully Refined Edible Oils
Hamide Filiz Ayyildiz, Mustafa Topkafa, Huseyin Kara, Syed Tufail Hussain Sherazi
International Journal of Food Properties
2. Current Awareness in Phytochemical Analysis
Phytochemical Analysis
3. Native Oils from Apple, Blackcurrant, Raspberry, and Strawberry Seeds as a Source of Polyenoic Fatty Acids, Tocochromanols, and Phytosterols: A Health Implication
Marek Pieszka, Władysław Migdał, Robert Gąsior, Magdalena Rudzińska, Dorota Bederska-Łojewska, Magdalena Pieszka, Paulina Szczurek
Journal of Chemistry
4. Heptane as a less toxic option than hexane for the separation of vitamin E from food products using normal phase HPLC
Oliver Buddrick, Oliver A. H. Jones, Paul D. Morrison, Darryl M. Small
RSC Advances
5. A rapid in-house validated GC-FID method for simultaneous determination of lipophilic bioactives in olive oil: Squalene, α-tocopherol, and β-sitosterol
Özge Seçmeler, Özlem Güçlü Üstündağ
European Journal of Lipid Science and Technology
6. Plant and Human Health, Volume 2
Umaiyal Munusamy, Siti Nor Akmar Abdullah
7. Handbook of Analysis of Active Compounds in Functional Foods
Severina Pacifico, Monica Scognamiglio, Brigida D’Abrosca, Pietro Monaco, Antonio Fiorentino
ISSN:1230-1388