ORIGINAL PAPER
Suitable method for simultaneous and specific
detection of maize (Zea mays l.) and genetically
modified soyabean (Glycine max l.) in animal feeds
1
Biosafety Laboratory, IMIDRA,
El Encín A-2 km 38.200. 28800 Alcalá de Henares, Spain
Publication date: 2009-01-23
Corresponding author
J. Anim. Feed Sci. 2009;18(1):162-172
KEYWORDS
ABSTRACT
Present method allows a simple and rapid identification of maize and specific transgenic soyabean
event in raw materials and processed feed samples. The method combines two aspects: suitable
DNA purification and amplification by means of multiplex RT-PCR. Efficiency and accuracy of
this method have been tested and the limit of detection (LOD) reached was of 0.1 ng/μl for one
of the species and of 1 ng/μl for the other one. The checking of the method has been realized by
means of RT-PCR using the specific probes for each one of the systems by means of comparison
with certified reference materials. The obtained results showed that with this method it is possible to
obtain, in a shorter time, quality values for both: identification and quantification of GMOs, in raw
and processed samples.
CITATIONS (1):
1.
Universal DNA-Based Methods for Assessing the Diet of Grazing Livestock and Wildlife from Feces
Anthony Pegard, Christian Miquel, Alice Valentini, Eric Coissac, Frédéric Bouvier, Dominique François, Pierre Taberlet, Erwan Engel, François Pompanon
Journal of Agricultural and Food Chemistry
Anthony Pegard, Christian Miquel, Alice Valentini, Eric Coissac, Frédéric Bouvier, Dominique François, Pierre Taberlet, Erwan Engel, François Pompanon
Journal of Agricultural and Food Chemistry
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