0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Suitable method for simultaneous and specific detection of maize (Zea mays l.) and genetically modified soyabean (Glycine max l.) in animal feeds

A. Gómez Garay 1  ,  
 
1
Biosafety Laboratory, IMIDRA, El Encín A-2 km 38.200. 28800 Alcalá de Henares, Spain
J. Anim. Feed Sci. 2009;18(1):162–172
Publish date: 2009-01-23
KEYWORDS
ABSTRACT
Present method allows a simple and rapid identification of maize and specific transgenic soyabean event in raw materials and processed feed samples. The method combines two aspects: suitable DNA purification and amplification by means of multiplex RT-PCR. Efficiency and accuracy of this method have been tested and the limit of detection (LOD) reached was of 0.1 ng/μl for one of the species and of 1 ng/μl for the other one. The checking of the method has been realized by means of RT-PCR using the specific probes for each one of the systems by means of comparison with certified reference materials. The obtained results showed that with this method it is possible to obtain, in a shorter time, quality values for both: identification and quantification of GMOs, in raw and processed samples.
CORRESPONDING AUTHOR
A. Gómez Garay   
Biosafety Laboratory, IMIDRA, El Encín A-2 km 38.200. 28800 Alcalá de Henares, Spain
ISSN:1230-1388