ORIGINAL PAPER
The apelin-13 influences the activity of pancreatic enzymes in young rats
| 1 | The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland |
| 2 | University of Life Sciences, Faculty of Veterinary Medicine, Department of Animal Physiology, Akademicka 12, 20-950 Lublin, Poland |
| 3 | University of Shizuoka, Laboratory of Physiology, 422-8526 Shizuoka, Japan |
| 4 | Warsaw University of Life Sciences - SGGW, Faculty of Veterinary Medicine, Department of Physiological Sciences, Nowoursynowska 166, 02-766 Warsaw, Poland |
CORRESPONDING AUTHOR
H. Antushevich
The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
The Kielanowski Institute of Animal Physiology and Nutrition Polish Academy of Sciences, Instytucka 3, 05-110 Jabłonna, Poland
Publication date: 2016-05-22
J. Anim. Feed Sci. 2016;25(2):160–166
KEYWORDS
ABSTRACT
Apelin and its APJ receptor are present inter alia in colostrum
and in the young animal gastrointestinal tract. This peptide exerts numerous
effects participating in the appetite and drinking behaviour, gastric acid
and insulin secretion. The aim of the study was to investigate the effect of
apelin-13 on the activity of pancreatic and gastric enzymes in young animals.
The two experiments were carried out on weaning Wistar rats (50 ± 10 g)
which received the apelin-13 or physiological saline (in the corresponding
control groups) by intragastric or intraperitoneal way twice a day for 10 days
(100 nmol · kg–1 body weight). At the end of each experiment rats were
sacrificed and blood samples were collected for apelin and cholecystokinin
(CCK) radioimmunoassay. The fragments of the pancreas and stomach were
weighted and frozen for the further digestive enzymes activity analysis. The
intragastric and intraperitoneal administration of apelin-13 increased plasma
CCK level in young rats. The intraperitoneal injection of apelin-13 stimulated
pancreatic trypsin, α-amylase and lipase activity, but had no effect on the
activity of gastric enzymes. On the other hand, the intragastric administration of
apelin-13 stimulated only the activity of pancreatic lipase and had no effect on
the activity of gastric pepsin and rennet. So, circulating apelin exerts the most
pronounced effect on pancreatic enzymes activity in young rats, but neither
circulating nor luminal apelin influences gastric enzymes activity. Regardless
the route of administration, apelin stimulates lipase activity which points out its
considerable role in the regulation of the fat digestion.
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CITATIONS (3):
1.
Peripheral apelin-13 administration inhibits gastrointestinal motor functions in rats: The role of cholecystokinin through CCK 1 receptor-mediated pathway
Mehmet Bülbül, Osman Sinen, İlknur Birsen, V. Nimet İzgüt-Uysal
Neuropeptides
Mehmet Bülbül, Osman Sinen, İlknur Birsen, V. Nimet İzgüt-Uysal
Neuropeptides
2.
Exogenous obestatin affects pancreatic enzyme secretion in rat
through two opposite mechanisms, direct inhibition and vagally-mediated stimulation
M. Kapica, I. Puzio, I. Kato, A. Kuwahara, R. Zabielski, H. Antushevich
Journal of Animal and Feed Sciences
M. Kapica, I. Puzio, I. Kato, A. Kuwahara, R. Zabielski, H. Antushevich
Journal of Animal and Feed Sciences
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