The influence of feeding raw vs extruded soyabean supplemented or unsupplemented with sulphur amino acids on performance, pancreatic trypsin activity and morphological parameters of the jejunum in rats
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Department of Physiology, Biochemistry, Pharmacology and Toxicology, Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warszawa, Poland
Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Warsaw Agricultural University, Nowoursynowska 166, 02-787 Warszawa, Poland
Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-718 Olsztyn, Poland
Publication date: 2001-06-28
J. Anim. Feed Sci. 2001;10(Suppl. 2):315–321
The effect of extrusion of soyabean seeds and sulphur amino acid (AA) supplementation on trypsin inhibitor (TI) and lectin activity, and on the histology and microbiology of the jejunum were studied. Wistar rats were fed semi-purified diets containing 10% of soyabean seeds: raw (R) or extruded at 160°C (E) and unsupplemented or supplemented with 0.3% (AA) (met+cys, 1:1). Extrusion reduced TI activity in seeds from 25.0 to 11.8 TUI/mg. Extrusion of soyabean seeds also reduced agglutination activity against human red blood cells from 1:1600 to 1:100 (group AB), from 1:800 to 1:100 (group 0), from 1:800 to 1:50 (group A), and from 1:400 to 1:50 (group B). Extrusion of soyabean seeds improved (P < 0.05) performance and decreased pancreas weight (0.40 vs 0.50% BW). Extrusion increased (P < 0.05) pancreatic trypsin activity (3495 vs 5901 U/100 g protein), improved the morphology of the jejunum, and reduced the E. coli count. The influence of AA in the group fed raw seeds on performance and morphological gut parameters was even better than the effect of extrusion.