ORIGINAL PAPER
Study on stability of fungal phytase as poultry feed additive
M. Karaman 1,   Y. Gurbuz 1,   E. Ozkose 1,   M. S. Ekinci 1  
 
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Kahramanmaras Sutcu Imam University, Faculty of Agriculture, Department of Animal Science, 46060 Kahramanmaras, Turkey
CORRESPONDING AUTHOR
M. S. Ekinci   

Kahramanmaras Sutcu Imam University, Faculty of Agriculture, Department of Animal Science, 46060 Kahramanmaras, Turkey
Publication date: 2004-04-09
 
J. Anim. Feed Sci. 2004;13(2):313–321
 
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ABSTRACT
Stability of phytase enzyme from Aspergillus niger against different pH values and proteases was studied to reveal its potential as poultry feed additive. Phytase enzyme lost approximately 53, 32, 66, and 87% of its activity at pH 1, 2, 8, and 9, respectively, but the enzyme was able to recover full activity at pH 5.5 following 6 h of exposure at 37°C to pH as low as 3. Pre-treatment of phytase enzyme with saliva for an hour resulted in lose of enzyme activity about 10%, and similarly treatment with proteases caused about 40% decrease of activity. Addition of phytase enzyme to the low phosphorus containing diets kept the tibia ash content constant. Supplementation of diet with phytase enzyme increased availability of total phosphorus. Phytase supplementation of broiler diets also improved growth and body weights and decreased mortality.
 
CITATIONS (4):
1.
Evaluation of increasing levels of a microbial phytase in phosphorus deficient broiler diets via live broiler performance, tibia bone ash, apparent metabolizable energy, and amino acid digestibility
J. Pieniazek, K. A. Smith, M. P. Williams, M. K. Manangi, M. Vazquez-Anon, A. Solbak, M. Miller, J. T. Lee
Poultry Science
 
2.
Effects of temperature, pH, incubation time and pepsin concentration on the in vitro stability of intrinsic phytase of wheat, barley and rye
O. Esmaeilipour, M.M. Van Krimpen, A.W. Jongbloed, L.H. De Jonge, P. Bikker
Animal Feed Science and Technology
 
3.
Application of the Taguchi method in poultry science: estimation of thein vitrooptimum intrinsic phytase activity of rye, wheat and barley
M. Sedghi, A. Golian, O. Esmaeilipour, M. M. Van Krimpen
British Poultry Science
 
4.
Bioprocessing of Agricultural Residues as Substrates and Optimal Conditions for Phytase Production of Chestnut Mushroom, Pholiota adiposa, in Solid State Fermentation
Kritsana Jatuwong, Jaturong Kumla, Nakarin Suwannarach, Kenji Matsui, Saisamorn Lumyong
Journal of Fungi
 
ISSN:1230-1388