ORIGINAL PAPER
Localization of kisspeptin neurons in the hypothalamus of peripubertal female lambs; possible connection with gonadotrophin releasing hormone and neuropeptide Y neurons
J. Polkowska 1  
,   S. Picard 2,   M. Wańkowska 1,   M. Cieślak 1,   A. Caraty 2,   Y. Tillet 2
 
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1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
2
Université François Rabelais de Tours, Unité Physiologie de la Reproduction et des Comportements, INRA Centre Val de Loire, CNRS, UMR7247, FED4226 Neuroimagerie Fonctionnelle, 37380 Nouzilly, France
CORRESPONDING AUTHOR
J. Polkowska   

The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Publication date: 2014-06-11
 
J. Anim. Feed Sci. 2014;23(2):139–148
 
KEYWORDS
ABSTRACT
Hypothalamic peptide kisspeptin (kp) plays a major role in the initiation of pubertal events in mammals through the activation of gonadotrophin-releasing hormone (GnRH) neurons. The present study was designed to analyse the morpho-functional role of kisspeptin cells in the multimodal effects on GnRH secretion in female prepubertal lambs. The localization of kp immunoreactive (ir) expression was compared with that of GnRH release and neuropeptide Y (NPY) synthesis. The experiment was conducted on eight 32-week-old Merino lambs. The distribution of the peptides was estimated using immunohistochemistry and anti-kp, anti-GnRH and anti-NPY specific antibodies. The hypothalamic area containing numerous kp-ir perikarya and a dense network of kp-ir fibres was localized in the caudal part of the arcuate nucleus. Single axons and single perikarya were observed throughout the hypothalamus extending from the preoptic area to the mammillary bodies. Distinct bundles of kp-ir nerve terminals were observed in the lateral zone of the median eminence (ME) and dispersed in smaller quantities in its medial zone. Double immunostaining indicated that a third of the kp-ir neurons were in close apposition with NPY-ir afferents, and that the distribution of kp-ir nerve terminals in the ME matched that of GnRH-ir nerve terminals. To conclude, the presence of very large numbers of kp-ir neurons in the peripubertal period could be attributed to their stimulating action on the GnRH neuronal system during this period. This influence could act directly in the ME, where both peptides are released. In addition, kp-ir neurons could mediate the effect of NPY on GnRH secretion.
 
CITATIONS (3):
1.
The effect of short fasting on the hypothalamic neuronal system of kisspeptin in peripubertal female lambs
Jolanta Polkowska, Magdalena Cieślak, Marta Wańkowska, Anna Wójcik-Gładysz
Animal Reproduction Science
 
2.
Expression of kisspeptin protein in hypothalamus and LH profile of growing female lambs
Jolanta Polkowska, Anna Wójcik-Gładysz, Natalia Chmielewska, Marta Wańkowska
Reproduction, Fertility and Development
 
3.
Obestatin may affect the GnRH/KNDy gene network in sheep hypothalamus
Anna Wójcik-Gładysz, Michał Szlis, Bartosz Jarosław Przybył, Jolanta Polkowska
Research in Veterinary Science
 
ISSN:1230-1388