0.917
IF5
1.024
IF
Q2
JCR
0.90
CiteScore
0.385
SJR
Q2
SJR
20
MNiSW
142.18
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Localization of kisspeptin neurons in the hypothalamus of peripubertal female lambs; possible connection with gonadotrophin releasing hormone and neuropeptide Y neurons

J. Polkowska 1  ,  
S. Picard 2,  
M. Wańkowska 1,  
M. Cieślak 1,  
A. Caraty 2,  
 
1
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
2
Université François Rabelais de Tours, Unité Physiologie de la Reproduction et des Comportements, INRA Centre Val de Loire, CNRS, UMR7247, FED4226 Neuroimagerie Fonctionnelle, 37380 Nouzilly, France
J. Anim. Feed Sci. 2014;23(2):139–148
Publish date: 2014-06-11
KEYWORDS:
ABSTRACT:
Hypothalamic peptide kisspeptin (kp) plays a major role in the initiation of pubertal events in mammals through the activation of gonadotrophin-releasing hormone (GnRH) neurons. The present study was designed to analyse the morpho-functional role of kisspeptin cells in the multimodal effects on GnRH secretion in female prepubertal lambs. The localization of kp immunoreactive (ir) expression was compared with that of GnRH release and neuropeptide Y (NPY) synthesis. The experiment was conducted on eight 32-week-old Merino lambs. The distribution of the peptides was estimated using immunohistochemistry and anti-kp, anti-GnRH and anti-NPY specific antibodies. The hypothalamic area containing numerous kp-ir perikarya and a dense network of kp-ir fibres was localized in the caudal part of the arcuate nucleus. Single axons and single perikarya were observed throughout the hypothalamus extending from the preoptic area to the mammillary bodies. Distinct bundles of kp-ir nerve terminals were observed in the lateral zone of the median eminence (ME) and dispersed in smaller quantities in its medial zone. Double immunostaining indicated that a third of the kp-ir neurons were in close apposition with NPY-ir afferents, and that the distribution of kp-ir nerve terminals in the ME matched that of GnRH-ir nerve terminals. To conclude, the presence of very large numbers of kp-ir neurons in the peripubertal period could be attributed to their stimulating action on the GnRH neuronal system during this period. This influence could act directly in the ME, where both peptides are released. In addition, kp-ir neurons could mediate the effect of NPY on GnRH secretion.
CORRESPONDING AUTHOR:
J. Polkowska   
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
 
CITATIONS (1):
1. The effect of short fasting on the hypothalamic neuronal system of kisspeptin in peripubertal female lambs
Jolanta Polkowska, Magdalena Cieślak, Marta Wańkowska, Anna Wójcik-Gładysz
Animal Reproduction Science
ISSN:1230-1388