Ligand-binding activity of growth hormone receptor (GH-R) in bulls of different breeds with identified GH-R genotypes
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Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, 05-551 Mroków, Poland
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
Publication date: 2002-05-09
J. Anim. Feed Sci. 2002;11(2):223–236
The relationship between the ligand-binding activity of growth hormone receptor (GH-R) and polymorphism Alul, Accl and Stul sites in the 5' flanking region of bovine GH-R gene was examined. The study was performed on twenty six 15-month old bulls of dairy (Friesians) and beef breeds (Charolais, Limousine, Piemontese, Aberdeen Angus, Hereford). Receptor binding capacity (Bmax) and dissociation constant (Kd) for GH-R were determined by the Scatchard method. The polymorphism in the GH-R gene was analyzed by PCR-RFLP technique. Our results showed significant differences in the liver GH-R Bmax and Kd between the dairy and beef breeds. Bmax was greater (P≤0.05) in Polish Friesians as compared to the beef breeds, while Kd revealed the lowest figure in the dairy breed (P≤0.01). No significant differences were found in the Bmax and Kd values between genotypes within the 5' flanking region of bovine GH-R gene. In conclusion, ligand-binding activity of the liver GH receptors was shown to differ between the dairy and beef bulls. Furthermore, the polymorphism in the 5' regions of GH-R gene seems to have no influence on ligand-binding properties of the liver GH receptor in cattle.
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