1.054
IF5
1.150
IF
Q3
JCR
1.7
CiteScore
0.396
SJR
Q2
SJR
40
MNiSW
148.75
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Ligand-binding activity of growth hormone receptor (GH-R) in bulls of different breeds with identified GH-R genotypes

R. Grochowska 1,  
A. Gajewska 2,  
M. Snochowski 2,  
 
1
Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, 05-551 Mroków, Poland
2
The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
J. Anim. Feed Sci. 2002;11(2):223–236
Publication date: 2002-05-09
KEYWORDS
ABSTRACT
The relationship between the ligand-binding activity of growth hormone receptor (GH-R) and polymorphism Alul, Accl and Stul sites in the 5' flanking region of bovine GH-R gene was examined. The study was performed on twenty six 15-month old bulls of dairy (Friesians) and beef breeds (Charolais, Limousine, Piemontese, Aberdeen Angus, Hereford). Receptor binding capacity (Bmax) and dissociation constant (Kd) for GH-R were determined by the Scatchard method. The polymorphism in the GH-R gene was analyzed by PCR-RFLP technique. Our results showed significant differences in the liver GH-R Bmax and Kd between the dairy and beef breeds. Bmax was greater (P≤0.05) in Polish Friesians as compared to the beef breeds, while Kd revealed the lowest figure in the dairy breed (P≤0.01). No significant differences were found in the Bmax and Kd values between genotypes within the 5' flanking region of bovine GH-R gene. In conclusion, ligand-binding activity of the liver GH receptors was shown to differ between the dairy and beef bulls. Furthermore, the polymorphism in the 5' regions of GH-R gene seems to have no influence on ligand-binding properties of the liver GH receptor in cattle.
 
CITATIONS (2):
1. Comparison of skeletal muscle transcriptional profiles in dairy and beef breeds bulls
T. Sadkowski, M. Jank, L. Zwierzchowski, J. Oprządek, T. Motyl
Journal of Applied Genetics
2. Diversity of growth hormone gene and its relation with average daily gain in Simmental cattle in West Sumatera Province, Indonesia
Suhada Hary, Anwar Sarbaini, Arnim, Maulana Hendri, Yurnalis Dan
African Journal of Biotechnology
ISSN:1230-1388