0.857
IF5
0.900
IF
Q3
JCR
0.92
CiteScore
0.405
SJR
Q2
SJR
20
MNiSW
165.24
ICV
ORIGINAL PAPER
 
CC-BY 4.0
 
 

Impact of contrasting fish oil concentrations in the diet on ovine embryo development in vivo and of corresponding diet-specific derivative sera during in vitro culture

T. G. McEvoy 1,  
A. G. Onal 1, 2,  
B. K. Speake 1,  
 
1
Sustainable Livestock Systems Research Group, Scottish Agricultural College, West Mains Road, Edinburgh, EH9 3JG, Scotland, United Kingdom
2
Present address: Department of Animal Science, Faculty of Agriculture, Mustafa Kemal University, Antakya, Hatay Province, Turkey
J. Anim. Feed Sci. 2012;21(1):31–48
Publish date: 2012-03-12
KEYWORDS
ABSTRACT
In the first of two experiments, numbers of ovulations and day 5 embryo yields were unaffected by fish oil (0, 3 or 6% w/w) in ewe diets; oestradiol concentrations and numbers of large ovarian follicles were increased. Fish oil tended to suppress embryo development and limit blastocyst diameter and cell number but not protein synthesis. In the second experiment IVF-derived embryos were cultured in medium supplemented with foetal calf serum (FCS) or, as in vitro equivalents to Experiment 1, serum from ewes fed rations supplemented with 0 (C), 3 (3F) or 6 (6F)% fish oil. Cleavage rates were not affected by serum source. More Grade 1 to 1.5 blastocysts were produced when using 3F serum (32% of blastocysts) than with C (27%; ns) or 6F (16%; P<0.05) or FCS (10%; P<0.005). It is suggested that at less than 3% inclusion in the diet of ewes, there may be beneficial effects of fish oil fatty acids on embryo production in vivo and of the corresponding ewe serum during in vitro culture. Higher concentrations are counter-productive.
CORRESPONDING AUTHOR
J. J. Robinson   
Sustainable Livestock Systems Research Group, Scottish Agricultural College, West Mains Road, Edinburgh, EH9 3JG, Scotland, United Kingdom
 
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ISSN:1230-1388