ORIGINAL PAPER
Identification of target sequences for association studies - analysis of the pig FABP3 and FABP4 loci using comparative genomics methods
A. Chmurzynska 1, 2,   J. Cieslak 1,   T. Jankowski 1,   M. Szydlowski 1,   M. Switonski 1  
 
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1
University of Life Sciences in Poznań, Department of Genetics and Animal Breeding, Wołyńska 33, Poznań 60-637, Poland
2
University of Life Sciences in Poznań, Department of Human Nutrition and Hygiene, Wołyńska 33, Poznań 60-637, Poland
CORRESPONDING AUTHOR
M. Switonski   

University of Life Sciences in Poznań, Department of Genetics and Animal Breeding, Wołyńska 33, Poznań 60-637, Poland
Publication date: 2008-04-08
 
J. Anim. Feed Sci. 2008;17(2):191–201
 
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ABSTRACT
Comparative genomics facilitates the identification of conserved and potentially functional elements. Their polymorphism can be responsible for phenotype variability. Two chromosomal regions were studied - one containing the FABP gene cluster and the second harbouring the FABP3 locus. Several highly conserved elements across four species (human, mouse, dog and cattle) were found, two of them were selected for association studies in the pig (the FABP4 enhancer and a fragment of intron 2 of the FABP3 gene). Four single nucleotide polymorphisms in the FABP4 enhancer sequence were found: 9 T/G, 31 C/G, 186 G/A, and 189 C/A. One of them (9T/G) was tested but the statistical analysis revealed no association with fatness traits in Polish Large White and Polish Landrace pigs. These studies demonstrate an integrated approach to identify, classify and select target DNA sequences for further association studies.
 
CITATIONS (4):
1.
Association studies on the porcine RETN, UCP1, UCP3 and ADRB3 genes polymorphism with fatness traits
Jakub Cieslak, Joanna Nowacka-Woszuk, Magdalena Bartz, Honorata Fijak-Nowak, Maria Grzes, Maciej Szydlowski, Marek Switonski
Meat Science
 
2.
Polymorphism of genes encoding cytokines IL6 and TNF is associated with pig fatness
M. Szydlowski, A. Buszka, M. Mackowski, D. Lechniak, M. Switonski
Livestock Science
 
3.
Genetics of fat tissue accumulation in pigs: a comparative approach
M. Switonski, M. Stachowiak, J. Cieslak, M. Bartz, M. Grzes
Journal of Applied Genetics
 
4.
Association of genetic variants and expression levels of porcine FABP4 and FABP5 genes
M. Ballester, A. Puig-Oliveras, A. Castelló, M. Revilla, A. I. Fernández, J. M. Folch
Animal Genetics
 
ISSN:1230-1388