ORIGINAL PAPER
β-carotene as a dietary factor affecting expression of genes connected with carotenoid, vitamin A and lipid metabolism in the subcutaneous and omental adipose tissue of beef cattle
C. Wei 1,   X. Tan 1,   G. Liu 1,   F. Wan 1,   H. Zhao 1,   C. Zhang 2,   W. You 1,   X. Liu 1,   X. Zhang 1,   Qing Jin 1  
 
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1
Shandong Academy of Agricultural Sciences, Shandong Key Lab of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Jinan, 250100 Shandong Province, China
2
Shandong Normal University, College of Life Sciences, Jinan, 250014 Shandong Province, China
CORRESPONDING AUTHOR
Qing Jin   

Shandong Academy of Agricultural Sciences, Shandong Key Lab of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Jinan, 250100 Shandong Province, China
Publication date: 2020-03-31
 
J. Anim. Feed Sci. 2020;29(1):11–18
 
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ABSTRACT
Adipose tissue in meat, especially subcutaneous fat, is not appreciated by consumers as it is considered unhealthy. The effects of β-carotene (βC) on the expression of ten genes related to carotenoid, vitamin A (VA) or lipid metabolism were evaluated in subcutaneous and omental (visceral) adipose tissue of Simmental crossbred steers receiving various βC treatments (0, 600, 1200 and 1800 mg/d). Two carotenoid oxidative cleavage genes (β-carotene- 15,15′-monooxygenase (BCMO1) and β-carotene-9′,10’-dioxygenase (BCO2)) were up-regulated and three VA metabolic genes (retinoid X receptor α (RXRA), retinal reductase (RALDH) and lecithin-retinol acyltransferase (LRAT)) were down-regulated in subcutaneous and omental adipose tissues. Gene encoding peroxisome proliferator-activated receptor γ (PPARG) involved in adipocyte differentiation and lipogenesis was down-regulated in both examined fat tissues. For the omental adipose tissue, the lipogenesis gene (fatty acid synthase (FAS)) and the two lipolysis genes (hormone-sensitive lipase (HSL) and adipose triglyceride lipase (ATGL)) were down-regulated, but another lipogenesis gene (acetyl-CoA carboxylase (ACC)) was up-regulated. First of all, the addition of βC in the diet may inhibit the expression of the major adipogenesis gene – PPARG, and increase the expression of genes involved in βC catabolism in adipose tissue of beef cattle. An effective βC dose to regulate the expression of genes connected with carotenoid, VA and lipid metabolism would be at least 600 mg/d.
 
REFERENCES (24)
1.
Amengual J., Gouranton E., van Helden Y.G.J. et al., 2011. Betacarotene reduces body adiposity of mice via BCMO1. PLoS ONE 6, e20644, https://doi.org/10.1371/journa....
 
2.
Boulanger A., McLemore P., Copeland N.G., Gilbert D.J., Jenkins N.A., Yu S.S, Gentleman S., Redmond T.M., 2003. Identification of beta-carotene 15,15’-monooxygenase as a peroxisome proliferator-activated receptor target gene. FASEB J. 17, 1304–1306, https://doi.org/10.1096/fj.02-....
 
3.
Condron K.N., Lemenager R.P., Claeys M.C., Lipkie T.E., Schoonmaker J.P., 2014. Supplemental β-carotene I: effect on plasma vitamin A, growth, performance, and carcass characteristics of feedlot cattle. Meat Sci. 98, 736–743, https://doi.org/10.1016/j.meat....
 
4.
Feng Y.L., 2000. The Nutrient Requirements and Feeding Standards of Beef Cattle. China Agricultural University Press. Beijing (China)
 
5.
García O.P., Long K.Z., Rosado J.L., 2009. Impact of micronutrient deficiencies on obesity. Nutr. Rev. 67, 559–572, https://doi.org/10.1111/j.1753....
 
6.
Gong X., Tsai S.W., Yan B., Rubin L.P., 2006. Cooperation between MEF2 and PPARγ in human intestinal β,β-carotene 15,15’-monooxygenase gene expression. BMC Mol. Biol. 7, 7, https://doi.org/10.1186/1471-2....
 
7.
Gorocica-Buenfil M.A., Fluharty F.L., Reynolds C.K., Loerch S.C., 2007. Effect of dietary vitamin A concentration and roasted soybean inclusion on marbling, adipose cellularity, and fatty acid composition of beef. J. Anim. Sci. 85, 2230–2242, https://doi.org/10.2527/jas.20....
 
8.
Jin Q., Cheng H., Wan F., Bi Y., Liu G., Liu X., Zhao H., You W., Liu Y., Tan X., 2015. Effects of feeding β-carotene on levels of β-carotene and vitamin A in blood and tissues of beef cattle and the effects on beef quality. Meat Sci. 110, 293–301, https://doi.org/10.1016/j.meat....
 
9.
Jin Q., Zhao H.B., Liu X.M., Wan F.C., Liu Y.F., Cheng H.J., You W., Liu G.F., Tan X.W., 2016. Effect of β-carotene supplementation on the expression of lipid metabolism-related genes and the deposition of back fat in beef cattle. Anim. Prod. Sci. 57, 513–519, https://doi.org/10.1071/AN1543....
 
10.
Lafontan M., Langin D., 2009. Lipolysis and lipid mobilization in human adipose tissue. Prog. Lipid Res. 48, 275–297, https://doi.org/10.1016/j.plip....
 
11.
Liu X., Fu J., Song E., Zang K., Wan F., Wu N., Wang A., 2009. Effect of nicotinamide on proliferation, differentiation, and energy metabolism in bovine preadipocytes. Asian-Austral. J. Anim. 22, 1320–1327, https://doi.org/10.5713/ajas.2....
 
12.
Liu X., Liu G., Tan X., Zhao H., Cheng H., Wan F., Wu N., Song E., 2014. Gene expression profiling of SIRT1, FoxO1, and PPARγ in backfat tissues and subcutaneous adipocytes of Lilu bulls. Meat Sci. 96, 704–711, https://doi.org/10.1016/j.meat....
 
13.
Liu X., Zhao H., Jin Q., You W., Cheng H., Liu Y., Song E., Liu G., Tan X., Zhang X., Wan F., 2018. Resveratrol induces apoptosis and inhibits adipogenesis by stimulating the SIRT1-AMPKα-FOXO1 signalling pathway in bovine intramuscular adipocytes. Mol. Cell Biochem. 439, 213–223, https://doi.org/10.1007/s11010....
 
14.
Livak K.J., Schmittgen T.D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔ(Ct) method. Methods 25, 402–408, https://doi.org/10.1006/meth.2....
 
15.
Lobo G.P., Amengual J., Palczewski G., Babino D., von Lintig J., 2012. Mammalian carotenoid-oxygenases: key players for carotenoid function and homeostasis. Biochim. Biophys. Acta 1821, 78–87, https://doi.org/10.1016/j.bbal....
 
16.
Mezaki Y., Fujimi T.J., Senoo H., Matsuura T., 2016. The coordinated action of lecithin: retinol acyltransferase and cellular retinol-binding proteins for regulation of vitamin A esterification. Med. Hypotheses 88, 60–62, https://doi.org/10.1016/j.mehy....
 
17.
Reynoso C.R., Mora O., Nieves V., Shimada A., González de Mejía E., 2004. β-carotene and lutein in forage and bovine adipose tissue in two tropical regions of Mexico. Anim. Feed Sci. Technol. 113, 183–190, https://doi.org/10.1016/j.anif....
 
18.
Schoof E., Stuppy A., Harig F., Carbon R., Horbach T., Stohr W., Rascher W., Dotsch J., 2004. Comparison of leptin gene expression in different adipose tissues in children and adults. Eur. J. Endocrinol. 150, 579–584, https://doi.org/10.1530/eje.0.....
 
19.
Shmarakov I.O., Yuen J.J., Blaner W.S., 2013. Carotenoid metabolism and enzymology. In: S.A. Tanumihardjo (Editor). Carotenoids and Human Health. Humana Press. Springer Science+Business Media. New York, NY (USA), pp. 29–56, https://doi.org/10.1007/978-1-....
 
20.
Siebert B.D., Kruk Z.A., Davis J., Pitchford W.S., Harper G.S., Bottema C.D.K., 2006. Effect of low vitamin A status on fat deposition and fatty acid desaturation in beef cattle. Lipids 41, 365–370, https://doi.org/10.1007/s11745....
 
21.
Tourniaire F., Gouranton E., von Lintig J., Keijer J., Luisa Bonet M., Amengual J., Lietz G., Landrier J.-F., 2009. β-Carotene conversion products and their effects on adipose tissue. Genes Nutr. 4, 179, https://doi.org/10.1007/s12263....
 
22.
Yang A., Larsen T.W., Tume R.K., 1992. Carotenoid and retinol concentrations in serum, adipose tissue and liver and carotenoid transport in sheep, goats and cattle. Aust. J. Agric. Res. 43, 1809–1817, https://doi.org/10.1071/AR9921....
 
23.
Zimmermann R., Strauss J.G., Haemmerle G. et al., 2004. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase. Science 306, 1383–1386, https://doi.org/10.1126/scienc....
 
24.
Ziouzenkova O., Orasanu G., Sukhova G., Lau E., Berger J.P., Tang G., Krinsky N.I., Dolnikowski G.G., Plutzky J., 2007. Asymmetric cleavage of β-carotene yields a transcriptional repressor of retinoid X receptor and peroxisome proliferator-activated receptor responses. Mol. Endocrinol. 21, 77–88, https://doi.org/10.1210/me.200....
 
 
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ISSN:1230-1388